Elucidation of molecular mechanism of the SufBCD complex responsible for biosynthesis of iron-sulfur cluster and development of its inhibitor
| Project/Area Number |
15H04472
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Saitama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
福山 恵一 大阪大学, 工学研究科, 招へい研究員 (80032283)
和田 啓 宮崎大学, 医学部, 准教授 (80379304)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | 鉄硫黄クラスター / 鉄硫黄タンパク質 / 生合成 / SUFマシナリー / SufBCD複合体 / 生合成マシナリー |
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| Outline of Final Research Achievements |
In this study, we have conducted a comprehensive analysis of the SUF machinery to gain insights into molecular mechanisms of the iron-sufur (Fe-S) cluster biosynthesis. (1) We have determined crystal structure of the SufBCD complex, and also clarified the mechanism of this complex by mutational and biochemical analyses. (2) We found that SufU and IscU are homologous proteins with different functions, and that SufU serves as a zinc-dependent sulfur carrier protein. These findings provide an essential understanding of the Fe-S cluster biosynthesis by the SUF machinery.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、SUFマシナリーにおけるFe-Sクラスターの生合成メカニズムについて、上記のように、鍵となる２つのポイントを世界に先駆けて明らかにすることができた。独自に研究の方向性を見定めて、オリジナルのin vivo実験系とin vitroの生化学的解析ならびに構造解析を有機的に関連付けて発展させた成果である。今後のFe-Sクラスター生合成研究の基盤となる重要な知見であり、学術的な意義は極めて大きい。
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Report
(5 results)
Research Products
(39 results)
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[Journal Article] A substrate-bound structure of cyanobacterial biliverdin reductase identifies stacked substrates as critical for activity2017
Author(s)
Haruna Takao, Kei Hirabayashi, Yuki Nishigaya, Haruna Kouriki, Tetsuko Nakaniwa, Yoshinori Hagiwara, Jiro Harada, Hideaki Sato, Toshimasa Yamazaki, Yoichi Sakakibara, Masahito Suiko, Yujiro Asada, Yasuhiro Takahashi, Ken Yamamoto, Keiichi Fukuyama, Masakazu Sugishima, and Kei Wada
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Journal Title
Nat. Commun.
Volume: 8
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Functional Dynamics Revealed by the Structure of the SufBCD Complex, a Novel ATP-binding Cassette (ABC) Protein That Serves as a Scaffold for Iron-Sulfur Cluster Biogenesis.2015
Author(s)
Hirabayashi K, Yuda E, Tanaka N, Katayama S, Iwasaki K, Matsumoto T, Kurisu G, Outten FW, Fukuyama K, Takahashi Y, Wada K.
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Journal Title
Journal of Biological Chemistry
Volume: 290
Issue: 50
Pages: 29717-29731
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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