A new twist of genome engineering technology by integrating CRISPR-Cas9 technology in yeast
| Project/Area Number |
15H04475
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Sojo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
浴野 圭輔 崇城大学, 生物生命学部, 准教授 (30310030)
杉山 峰崇 大阪大学, 工学研究科, 准教授 (80379130)
笹野 佑 崇城大学, 生物生命学部, 准教授 (90640194)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | 酵母 / ゲノム工学 / CRISPR/Cas9 / 合成致死 / 育種学 / 応用微生物 / ゲノム / バイテクノロジー / バイオテクノロジー / 染色体工学 / ゲノムの再編成工学 / ゲノム育種 |
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| Outline of Final Research Achievements |
Genome engineering could be a prospective research-promoting engine for both basic and applied biosciences. With this basic idea, we have developed a variety of genome engineering technologies. These include one-step chromosome splitting, deletion, segmental duplication and chromosome replacement. In this study, we have further developed these genome engineering technologies by integrating CRISPR-Cas9 genome editing technology. Outcome of these efforts resulted in multiple simultaneous splitting, deletion and duplication of any desired site and regions on tchromosome. These achievement accelerates manipulation of genome to reveal not only genome function but also strain breeding for biotechnology.
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Report
(4 results)
Research Products
(39 results)
