| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Outline of Final Research Achievements |
Genome engineering could be a prospective research-promoting engine for both basic and applied biosciences. With this basic idea, we have developed a variety of genome engineering technologies. These include one-step chromosome splitting, deletion, segmental duplication and chromosome replacement. In this study, we have further developed these genome engineering technologies by integrating CRISPR-Cas9 genome editing technology. Outcome of these efforts resulted in multiple simultaneous splitting, deletion and duplication of any desired site and regions on tchromosome. These achievement accelerates manipulation of genome to reveal not only genome function but also strain breeding for biotechnology.
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