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Variety and regulation of lanthanide-dependent methanol dehydrogenases

Research Project

Project/Area Number 15H04476
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionOkayama University

Principal Investigator

Tani Akio  岡山大学, 資源植物科学研究所, 准教授 (00335621)

Co-Investigator(Renkei-kenkyūsha) MITSUI Ryoji  岡山理科大学, 理学部生物科学科, 教授 (60319936)
NAKAGAWA Tomoyuki  岐阜大学, 応用生物科学部, 教授 (70318179)
Research Collaborator LV Haoxin  岡山大学, 資源植物科学研究所
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywordsメタノール / ランタノイド / メチロトローフ / Methylotroph / methanol / lanthanide / 微生物酵素 / 応用微生物
Outline of Final Research Achievements

We genetically analyzed the function of multiple methanol dehydrogenase (MDH) -like genes in Methylobacterium species. It was found that Ca-dependent MDH, MxaF and Ln-dependent MDH, XoxF1 were necessary for the growth on methanol and ethanol. Another alcohol dehydrogenase is involved in propanol oxidation. In addition, genetic analysis on MxbD that acts as lanthanide-switch for the expression of mxaF and xoxF has been done. MxbD is necessary for mxaF expression but not for xoxF expression, and spontaneous mutation in HAMP domain in the protein releases the repression of mxaF expression by the presence of XoxF.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (36 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (12 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 11 results,  Open Access: 11 results) Presentation (21 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results)

  • [Int'l Joint Research] ムグラ大学(トルコ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ジョモケニアッタ農工大学(ケニア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ブラパー大学(タイ)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Distribution of 1-aminocyclopropane-1-carboxylate deaminase and D-cysteine desulfhydrase genes among type species of the genus Methylobacterium.2018

    • Author(s)
      Ekimova GA, Fedorov DN, Tani A, Doronina NV, Trotsenko YA.
    • Journal Title

      Antonie Van Leeuwenhoek

      Volume: - Issue: 10 Pages: 1-12

    • DOI

      10.1007/s10482-018-1061-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation and genomic characterization of Novimethylophilus kurashikiensis gen. nov. sp. nov., a new lanthanide-dependent methylotrophic species of Methylophilaceae.2018

    • Author(s)
      Lv H, Sahin N, Tani A
    • Journal Title

      Environ Microbiol

      Volume: 20 Issue: 3 Pages: 1204-1223

    • DOI

      10.1111/1462-2920.14062

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lanthanide-Dependent Regulation of Methylotrophy in Methylobacterium aquaticum Strain 22A2018

    • Author(s)
      Masuda Sachiko、Suzuki Yutaka、Fujitani Yoshiko、Mitsui Ryoji、Nakagawa Tomoyuki、Shintani Masaki、Tani Akio
    • Journal Title

      mSphere

      Volume: 3 Issue: 1

    • DOI

      10.1128/msphere.00462-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cultivable Methylobacterium species diversity in rice seeds identified with whole-cell matrix-assisted laser desorption / ionization time-of-flight mass spectrometric analysis2018

    • Author(s)
      Okumura M, Fujitani Y, Maekawa M, Charoenpanich J, Murage H, Kimbara K, Sahin N, Tani A
    • Journal Title

      J. Biosci. Bioeng

      Volume: 123 Issue: 2 Pages: 190-196

    • DOI

      10.1016/j.jbiosc.2016.09.001

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Oharaeibacter diazotrophicus gen. nov., sp. nov., a diazotrophic and facultatively methylotrophic bacterium, isolated from rice rhizosphere2017

    • Author(s)
      Lv HX, Masuda S, Fujitani Y, Sahin N, Tani A
    • Journal Title

      Int J Syst Evol Microbiol

      Volume: 67 Issue: 3 Pages: 576-582

    • DOI

      10.1099/ijsem.0.001660

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation and characterization of thermotolerant ethanol-fermenting yeasts from Laos and application of whole-cell MALDI-TOF/MS analysis for their quick identification.2016

    • Author(s)
      Keo-oudone C, Nitiyon S, Sotitham P, Tani A, Noppon L, Yuangsaard N, Bounphanmy S, Limtong S, Yamada M
    • Journal Title

      Afr J Biotechnol 15, 153-164 (2016)

      Volume: 15 Issue: 6 Pages: 153-164

    • DOI

      10.5897/ajb2015.14984

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A capsidless ssRNA virus hosted by an unrelated dsRNA virus2016

    • Author(s)
      Zhang, R., Hisano, S., Tani, A., Kondo, H., Kanematsu, S., and Suzuki, N.
    • Journal Title

      Nature Nicrobiology

      Volume: 1 Issue: 1 Pages: 15001-15001

    • DOI

      10.1038/nmicrobiol.2015.1

    • NAID

      120007004850

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 植物葉面に共生するMethylobacterium属細菌の特性2016

    • Author(s)
      谷 明生
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 74 Pages: 135-137

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Complete genome sequence of Methylobacterium aquaticum strain 22A, isolated from Racomitrium japonicum moss.2015

    • Author(s)
      Tani A., Ogura Y., Hayashi T., Kimbara K.
    • Journal Title

      Genome Announc

      Volume: 3 Issue: 2

    • DOI

      10.1128/genomea.00266-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methylobacterium species promoting rice and barley growth and interaction specificity revealed with whole-cell matrix-assisted laser desorption / ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) analysis.2015

    • Author(s)
      Tani A, Sahin N, Fujitani Y, Kato A, Sato K
    • Journal Title

      PLoS ONE

      Volume: 10 Issue: 6 Pages: e0129509-e0129509

    • DOI

      10.1371/journal.pone.0129509

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Novel Metabolic Pathway Requiring Rare-Earth Elements as an Essential Factor2015

    • Author(s)
      中川智行,三井亮司,谷明生,河合啓一
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 53 Issue: 11 Pages: 744-750

    • DOI

      10.1271/kagakutoseibutsu.53.744

    • NAID

      130005431477

    • ISSN
      0453-073X, 1883-6852
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of ergothioneine by Methylobacterium species.2015

    • Author(s)
      Alamgir K. Masuda S, Fujitani Y, Fukuda F, Tani A
    • Journal Title

      Front. Microbiol. 6, 1185 (2015)

      Volume: 6 Pages: 1185-1185

    • DOI

      10.3389/fmicb.2015.01185

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Methylobacterium 属細菌におけるメタノール走化性センサーの機能解析2018

    • Author(s)
      春名優希、加藤純一、谷 明生
    • Organizer
      日本農芸化学 会 名古屋 名城大学
    • Related Report
      2017 Annual Research Report
  • [Presentation] Methylobacterium aquaticum strain 22A に おけるメタノール脱水素酵素遺伝子のラン タノイド依存的制御2018

    • Author(s)
      平賀翔大、藤谷良子、谷 明生
    • Organizer
      日本農芸化学 会 名古屋 名城大学
    • Related Report
      2017 Annual Research Report
  • [Presentation] Draft genome and description of Novimethylophilus kurashikiensis gen. nov. sp. nov., a new lanthanide-dependent methylotrophic species of Methylophilaceae2018

    • Author(s)
      Haoxin Lyu and Akio Tani
    • Organizer
      日本農芸化学 会 名古屋 名城大学
    • Related Report
      2017 Annual Research Report
  • [Presentation] Methylobacterium属細菌におけるメタノール走化性センサーの同定2017

    • Author(s)
      伊賀俊貴、加藤純一、谷 明生
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 根粒菌Bradyrhizobium diazoefficiens由来レアアース依存的メタノール脱水素酵素の酵素科学的性質2017

    • Author(s)
      菅沼宗矢,王倫,日比野歩美,三井亮司,谷明生,海老原章郎,早川享志,中川智行
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Genomic characterization of a new methylotroph, Oharaeibacter diazotrophicus SM302017

    • Author(s)
      Haoxin Lyu, Akio Tani
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Methylobacterium 属細菌におけるメタノー ル走化性センサーの同定2017

    • Author(s)
      伊賀俊貴、加藤純一、谷 明生
    • Organizer
      日本農芸化学会 2017 年度大会 京都 京都女子大
    • Related Report
      2017 Annual Research Report
  • [Presentation] Genomic characterization of a new methylotroph, Oharaeibacter diazotrophicus SM302017

    • Author(s)
      H. Lv, A. Tani
    • Organizer
      日本農芸化学会 2017 年度大会 京都 京都女子大
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物共生メタノール資化性細菌 Methylobacterium 属細菌におけるエルゴチオネイン生産と役割2017

    • Author(s)
      谷 明生
    • Organizer
      環境微生物系合同大会 公募シンポ6
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Methylophilus sp. La2-4, a lanthanide-dependent methanol-utilizing bacterium isolated from rice rhizosphere soil2017

    • Author(s)
      Lv HX, Sahin N, Tani A
    • Organizer
      IUMS2017, Singapore
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 低Ca環境でもメタノールに生育できるMethylobacterium zatmaniiのメタノール脱水素酵素の性質について2016

    • Author(s)
      王 倫,菅沼 宗矢,谷 明生,早川 享志,中川 智行
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Bradyrhizobium diazoefficiens USDA110由来REE依存型メタノール脱水素酵素の酵素化学的諸性質2016

    • Author(s)
      菅沼宗矢, 王 倫, 日比野歩美, 三井亮司, 谷 明生, 海老原章郎, 早川享志, 中川智行
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物共生Methylobacterium属細菌の生態2016

    • Author(s)
      谷 明生
    • Organizer
      第7回農芸化学の未来開拓セミナー
    • Place of Presentation
      岡山
    • Year and Date
      2016-05-20
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Methylobacterium 属細菌におけるメタノール走化性センサーの同定2016

    • Author(s)
      伊賀俊貴、加藤純一、谷 明生
    • Organizer
      日本農芸化学会2016
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Screening of lanthanu-dependent methylotrophs2016

    • Author(s)
      呂好新、谷 明生
    • Organizer
      日本農芸化学会2016
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Methylobacterium aquaticum 22Aのメタノール脱水素酵素ホモログの機能解析2016

    • Author(s)
      中辻由加里、谷 明生
    • Organizer
      日本農芸化学会2016
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 植物共生細菌Methylobacterium zatmaniiの重希土類元素およびCaに対する生育特性とメタノール脱水素酵素の性質2016

    • Author(s)
      王 倫,菅沼 宗矢,谷 明生,早川 享志,中川 智行
    • Organizer
      日本農芸化学会中部支部
    • Place of Presentation
      名古屋
    • Related Report
      2016 Annual Research Report
  • [Presentation] 根粒菌Bradyrhizobium diazoefficiensは新奇なレアアース依存的メタノール代謝系をもつ2016

    • Author(s)
      菅沼宗矢,王倫,日比野歩美,三井亮司,谷明生,海老原章郎,早川享志,中川智行
    • Organizer
      日本農芸化学会中部支部
    • Place of Presentation
      名古屋
    • Related Report
      2016 Annual Research Report
  • [Presentation] Methylobacterium extorquens AM1のメタノールデヒドロゲナーゼアイソザイムが植物共生に果たす役割2015

    • Author(s)
      一小路貴士, 峰松由季, 中川智行, 谷明生, 田中三男, 三井亮司
    • Organizer
      生物工学会2015
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Methylobacterium属細菌によるエルゴチオネインの生産2015

    • Author(s)
      Kabir Md Alamgir, 増田幸子, 谷 明生
    • Organizer
      生物工学会2015
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Methylobacterium属細菌と植物との共生相互作用』2015

    • Author(s)
      谷 明生
    • Organizer
      第6回NAMEフォーラム
    • Place of Presentation
      愛媛大学
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
    • Invited

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Published: 2015-04-16   Modified: 2019-03-29  

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