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Study on plant targetting mechanism of soil-borne plant pathogen Ralstonia solanacearum

Research Project

Project/Area Number 15H04478
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionHiroshima University

Principal Investigator

KATO Junichi  広島大学, 先端物質科学研究科, 教授 (90231258)

Co-Investigator(Renkei-kenkyūsha) TAJIMA Takahisa  広島大学, 大学院先端物質科学研究科, 助教 (80571116)
KAWASAKI Takeru  広島大学, 大学院先端物質科学研究科, 助教 (00510299)
Research Collaborator VANGNAI Alisa S.  チュラロンコン大学, 理学部, 准教授
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords走化性 / 青枯病菌 / 物質認識機構 / 植物感染 / 植物-細菌相互作用 / 走化性センサー / 青枯病 / Ralstonia solanacearum / 生物間相互作用 / バイオテクノロジー / 植物病原菌 / 生物相互作用 / シグナル伝達 / 植物-微生物相互作用
Outline of Final Research Achievements

Bacteria continuously sense chemical concentrations around cells and swim to their favorite sites or away from unfavorable sites. This behavioral responses are called chemotaxis. Ralstonia solanacearum, a soil-borne plant pathogen, locates its host plants by moving in soil. Chemotaxis is thought to have an important role in this early stage of infection by R. solanacearum. In this study, we have investigated molecular biology of R. solanacearum chemotaxis to understand how it locates rhizosphere of host plants and how chemotaxis is involved in plant infection. Methyl bromide is effective for controlling R. solanacearum infection, but its usage have been banned because it is a very strong ozone-depleting substance. It is highly expected that this study provides information valuable to establish environmentally-friendly controlling method of R. solanacearum wilt, which is independent on methyl bromide.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (25 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (6 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 8 results,  Invited: 3 results) Remarks (1 results)

  • [Int'l Joint Research] Chulalongkorn University(タイ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Chulalongkorn University(Thailand)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Chulalongkorn University(Thailand)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 土壌伝染性青枯病菌の植物認識機構-走化性の徹底解明-2018

    • Author(s)
      緋田安希子、奥正太、田島誉久、加藤純一
    • Journal Title

      アグリバイオ

      Volume: 2 Pages: 56-59

    • NAID

      40021807558

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Involvement of many chemotaxis sensors in negative chemotaxis to ethanol in Ralstonia pseudosolanacearum Ps292017

    • Author(s)
      Oku Shota、Hida Akiko、Mattana Tunchai、Tajima Takahisa、Nakashimada Yutaka、Kato Junichi
    • Journal Title

      Microbiology

      Volume: 163 Issue: 12 Pages: 1880-1889

    • DOI

      10.1099/mic.0.000574

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of boric acid as a novel chemoattractant and elucidation of its chemoreceptor in Ralstonia pseudosolanacearum Ps292017

    • Author(s)
      Hida Akiko、Oku Shota、Nakashimada Yutaka、Tajima Takahisa、Kato Junichi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 8609-8609

    • DOI

      10.1038/s41598-017-09176-3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Negative chemotaxis of Ralstonia pseudosolanacearum to maleate and identification of the maleate chemosensory protein2017

    • Author(s)
      Tunchai Mattana、Hida Akiko、Oku Shota、Nakashimada Yutaka、Nikata Toshiyuki、Tajima Takahisa、Kato Junichi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 124 Issue: 6 Pages: 647-652

    • DOI

      10.1016/j.jbiosc.2017.07.002

    • NAID

      40021420076

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of chemosensors for d-malate, unnatural enantiomer of malate, in Ralstonia pseudosolanacearum.2017

    • Author(s)
      Mattana Tunchai, Akiko Hida, Shota Oku, Yutaka Nakashimada, Takahisa Tajima, Junichi Kato
    • Journal Title

      Microbiology

      Volume: 163 Issue: 2 Pages: 233-242

    • DOI

      10.1099/mic.0.000408

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Identification of the mcpA and mcpM genes, encoding methyl-accepting proteins involved in amino acid and l-malate chemotaxis, and involvement of McpM-mediated chemotaxis in plant infection by Ralstonia pseudosolanacearum (formerly Ralstonia solanacearum phylotypes I and III)2015

    • Author(s)
      A. Hida, S. Oku, T. Kawasaki, Y. Nakashimada, T. Tajima, J. Kato
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 81 Issue: 21 Pages: 7420-7430

    • DOI

      10.1128/aem.01870-15

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Ralstonia solanacearumの走化性センサー遺伝子の機能発現に関する研究2017

    • Author(s)
      竹下 正記, 緋田 安希子, Mattana Tunchai, 田島 誉久, 中島田 豊, 加藤 純一
    • Organizer
      日本生物工学会第69回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 青枯病菌Ralstonia solanacearumのクエン酸走化性と植物感染への重要性2017

    • Author(s)
      緋田 安希子, Tunchai Mattana, 田島 誉久, 中島田豊, 加藤 純一
    • Organizer
      日本生物工学会第69回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 植物成長促進根圏細菌Pseudomonas protegens CHA0のアミノ酸走化性センサー群の特性化2017

    • Author(s)
      松田 浩希, 緋田 安希子, 田島 誉久, 加藤 純一
    • Organizer
      日本生物工学会第69回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 青枯病菌の感染最初期段階における走化性の役割2016

    • Author(s)
      加藤純一、奥正太、緋田安希子、M. Tunchai、田島誉久
    • Organizer
      日本細菌学会2016年度大会
    • Place of Presentation
      大阪府大阪市
    • Year and Date
      2016-03-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Identification and characterization of chemotactic transducers for a non-natural enantiomer of malate in Ralstonia solanacearum.2016

    • Author(s)
      Tunchai M, Hida A, Oku S, Nakashimada Y, Tajima T, Kato J
    • Organizer
      International Biotechnology Symposium & Exhibition 2016
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular characterization of chemotaxis involved in plant infection in Ralstonia solanacearum.2016

    • Author(s)
      Kato J, Hida A, Oku S, Tunchai M, Vangnai AS, Tajima T
    • Organizer
      International Biotechnology Symposium & Exhibition 2016
    • Place of Presentation
      Melbourne, Australia
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular characterization of chemotaxis involved in plant infection in Ralstonia solanacearum.2016

    • Author(s)
      Kato J, Vangnai AS, Hida A, Oku S, Tunchai M, Tajima T
    • Organizer
      The 3rd Sattellite Seminar of Core to Core Program
    • Place of Presentation
      Can Tho, Vietnam
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Plant-growth promoting Pseudomonas fluorescens MC46: Role on rhizoremediation.2016

    • Author(s)
      Sompornpailin D, Teacomporn P, Sipahuntar M, Kato J, Vangnai, AS
    • Organizer
      The 2nd Joint Seminar Core to Core Program
    • Place of Presentation
      Chonburi, Thailand
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel method to control Ralstonia solanacearum plant infection by disturbing its ability to locate host plants.2016

    • Author(s)
      Kato J, Vangnai AS, Hida A, Tunchai M, Tajima T
    • Organizer
      The 2nd Joint Seminar Core to Core Program
    • Place of Presentation
      Chonburi, Thailand
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Chemotaxis in Ralstonia solanacearum and its involvement in plant infection2015

    • Author(s)
      Kato, J., Hida, A., Oku, S., Tunchai, M., Tajima, T., Vangnai, A.
    • Organizer
      Asian Congress on Biotechnology
    • Place of Presentation
      Kuala Lumpur, Malaysia
    • Year and Date
      2015-11-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 複数の走化性リガンドが存在する時の走化性応答2015

    • Author(s)
      鳥越美玖、奥正太、緋田安希子、田島誉久、中島田豊、加藤純一
    • Organizer
      日本生物工学会2015年度大会
    • Place of Presentation
      鹿児島県鹿児島市
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Ralstonia solanacearumの負の走化性2015

    • Author(s)
      奥正太、緋田安希子、M. Tunchai、田島誉久、中島田豊、加藤純一
    • Organizer
      日本生物工学会2015年度大会
    • Place of Presentation
      鹿児島県鹿児島市
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Identification of chemoreceptor for D-malic acid, an unnatural occurred form, in Ralstonia solanacearum2015

    • Author(s)
      Tunchai, M., Oku, S., Hida, A., Tajima, T., Nakashimada, Y., Kato, J.
    • Organizer
      7th JKT (Japan-Korea-Taiwan) International Symposium on Microbial Ecology
    • Place of Presentation
      Tsuchiura, Ibaragi, Japan
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ralstonia solanacearumの忌避物質に対する走化性2015

    • Author(s)
      奥正太、緋田安希子、M. Tunchai、中島田豊、田島誉久、加藤純一
    • Organizer
      日本微生物生態学会2015年度大会
    • Place of Presentation
      茨城県土浦市
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Chemotaxis in Ralstonia solanacearum and its involvement in plant infection2015

    • Author(s)
      Kato, J., Hida, A., Oku, S., Tunchai, M., Tajima, T., Vangnai, A.
    • Organizer
      International Joint Seminar of New Core to Core Program A. Advanced Research Network
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2015-08-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] 広島大学大学院先端物質科学研究科分子生命機能科学専攻加藤研究室

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/kato_lab/chemotaxis.html

    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2022-09-27  

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