| Budget Amount *help |
¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
It has long been known that bifidobaceria-rich microbiota (bifidus flora) is formed in breast-fed infant intestines; however the underlying mechanism has not been elucidated. We have found that infant gut-associated bifidobacteria have an ability to assimilate human milk oligosaccharides (HMOs) that comprises the third most abundant solid component. In this study, we focused on lacto-N-biosidase, which hydrolyzes lacto-N-tetraose (the most abundant core HMO structure ), and revealed that the enzyme plays an important role in bifidus flora formation in infants. In addition, we succeeded in developing an efficient method to synthesize lacto-N-tetraose by mutating a relevant enzyme. The compound can be used for formula milk fortification.
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