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Functional analysis of triterpenoid biosynthetic genes in plant by using genome editing

Research Project

Project/Area Number 15H04485
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionOsaka University

Principal Investigator

Muranaka Toshiya  大阪大学, 工学研究科, 教授 (60342862)

Co-Investigator(Kenkyū-buntansha) 福島 エリオデット  大阪大学, 工学研究科, 助教 (40724448)
Co-Investigator(Renkei-kenkyūsha) SEKI Hikaru  大阪大学, 大学院工学研究科, 准教授 (30392004)
Research Collaborator YASUMOTO Shuhei  
SUZUKI Hayato  
TAMURA Keita  
ROBERTLEE Jekson  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywordsゲノム編集 / 植物組織培養 / テルペノイド / 代謝制御 / 植物特化代謝物 / 合成生物学 / P450 / トリテルペノイド / 組織培養 / バイオテクノロジー / 植物 / 代謝工学 / トマト / シロイヌナズナ / カンゾウ / 酵母
Outline of Final Research Achievements

Plants produce a wide variety of secondary (specialized) metabolites to survive under biotic and abiotic stress. Among them, terpenoids are those of the most structurally varied class of plant specialized metabolites and perform multiple important biological roles. Cytochrome P450s (CYPs) have a central role to enhance the diversity of the triterpenoids, C-30 terpenoids. In this study, we analyzed the function of various CYPs in triterpenoid biosynthesis using yeast heterologous expression system. Furthermore, we created gene knockout plant/plant tissues to confirm in planta function of those CYPs including Arabidoisis, tomato and licorice.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (52 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (5 results) Journal Article (11 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 10 results,  Open Access: 5 results,  Acknowledgement Compliant: 5 results) Presentation (30 results) (of which Int'l Joint Research: 10 results,  Invited: 20 results) Book (4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] 上海交通大学(中国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] マヒドン大学(タイ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] コペンハーゲン大学(デンマーク)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] コペンハーゲン大学(デンマーク)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] コペンハーゲン大学(デンマーク)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Plant-derived isoprenoid sweeteners: recent progress in biosynthetic gene discovery and perspectives on microbial production2018

    • Author(s)
      Seki Hikaru、Tamura Keita、Muranaka Toshiya
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: in press Issue: 6 Pages: 927-934

    • DOI

      10.1080/09168451.2017.1387514

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of the soybean Sg-3 gene that is involved in genetic variation in sugar chain composition at the C-3 position in soyasaponin2018

    • Author(s)
      Yano R, Takagi K, Tochigi S, Fujisawa Y, Nomura Y, Tsuchinaga H, Takahashi Y, Takada Y, Kaga A, Anai T, Tsukamoto C, Seki H, Muranaka T, Ishimoto M
    • Journal Title

      Plant Cell Physiol.

      Volume: 59 Issue: 4 Pages: 797-810

    • DOI

      10.1093/pcp/pcy075

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence that the <i>Arabidopsis thaliana</i> 3-hydroxy-3-methylglutaryl-CoA reductase 1 is phosphorylated at Ser577 <i>in planta</i>2018

    • Author(s)
      Robertlee J, Kobayashi K, Tang J, Suzuki M, Muranaka T
    • Journal Title

      Plant Biotechnology

      Volume: 35 Issue: 1 Pages: 1-7

    • DOI

      10.5511/plantbiotechnology.17.1208a

    • NAID

      130006727678

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure and hemolytic activity relationships of triterpenoid saponins and sapogenins2017

    • Author(s)
      Vo N. N. Q., Fukushima E.O., Muranaka T.
    • Journal Title

      J. Nat. Med.

      Volume: 71 Issue: 1 Pages: 50-58

    • DOI

      10.1007/s11418-016-1026-9

    • NAID

      40021062562

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume2017

    • Author(s)
      Keiichi Mochida, Tetsuya Sakurai, Hikaru Seki, Takuhiro Yoshida, Kotaro Takahagi, Satoru Sawai, Hiroshi Uchiyama, Toshiya Muranaka, Kazuki Saito
    • Journal Title

      The Plant Journal

      Volume: 89 Issue: 2 Pages: 181-194

    • DOI

      10.1111/tpj.13385

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis.2017

    • Author(s)
      Tamura, K., Seki, H., Suzuki, H., Kojoma, M., Saito, K. and Muranaka, T.: CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis.
    • Journal Title

      Plant Cell Rep.

      Volume: 36 Issue: 3 Pages: 437-445

    • DOI

      10.1007/s00299-016-2092-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Functional characterization of CYP716 family P450 enzymes in triterpenoid biosynthesis in tomato2017

    • Author(s)
      Shuhei Yasumoto, Hikaru Seki, Yuko Shimizu, Ery O. Fukushima and Toshiya Muranaka
    • Journal Title

      Frontiers in Plant Science

      Volume: 8 Pages: 1-13

    • DOI

      10.3389/fpls.2017.00021

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Platform for &#x201c;Chemical Metabolic Switching&#x201d; to Increase Sesquiterpene Content in Plants2017

    • Author(s)
      K. Kobayashi, K. Kobayashi, H. Yamaguchi, Y. M. Inoue, K. Takagi, K. Fushihara, H. Seki, M. Suzuki, N. Nagata, T. Muranaka
    • Journal Title

      Plant Biotechnology

      Volume: 34 Issue: 1 Pages: 65-69

    • DOI

      10.5511/plantbiotechnology.17.0114a

    • NAID

      130005519610

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] AKIN10, a representative Arabidopsis SNF1-related protein kinase 1 (SnRK1), phosphorylates and downregulates plant HMG-CoA reductase2017

    • Author(s)
      Robertlee J, Kobayashi K, Suzuki M and Muranaka T
    • Journal Title

      FEBS Letters

      Volume: 印刷中 Issue: 8 Pages: 1159-1166

    • DOI

      10.1002/1873-3468.12618

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 農作物でのゲノム編集2016

    • Author(s)
      安本周平、村中俊哉
    • Journal Title

      実験医学

      Volume: 34 Pages: 3410-3415

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Novel triterpene oxidizing activity of Arabidopsis thaliana CYP716A subfamily enzymes2016

    • Author(s)
      Shuhei Yasumoto, Ery O. Fukushima, Hikaru Seki and Toshiya Muranaka
    • Journal Title

      FEBS Letters

      Volume: 590 Issue: 4 Pages: 533-540

    • DOI

      10.1002/1873-3468.12074

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Redesign of terpenoid biosynthetic pathway in plant by genome editing2017

    • Author(s)
      Muranaka T
    • Organizer
      TERPNET2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物におけるゲノム編集技術とスマートアグリに向けて2017

    • Author(s)
      村中俊哉
    • Organizer
      諏訪東京理科大学公開シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Redesign of biosynthetic pathway in plant by genome editing for human health and food supply2017

    • Author(s)
      Muranaka T
    • Organizer
      JSPS-AvH Alumni Joint Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物のゲノム編集について共に学ぶ2017

    • Author(s)
      村中俊哉
    • Organizer
      第3回デザイン思考勉強会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Redesign of terpenoid biosynthetic pathway in plant by genome editing toward human health2017

    • Author(s)
      Muranaka T
    • Organizer
      MU-OU 15th Anniversary symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Introduction: "Smart cell industry" a new trend in bioeconomy2017

    • Author(s)
      Muranaka T
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Effect of LjCYP93E disruption on triterpenoid content in Lotus japonicus plants, The 13th International Meeting on Biosynthesis, Function and Synthetic Biology of Isoprenoids2017

    • Author(s)
      Suzuki H, Fukushima EO, Seki H, Muranaka T
    • Organizer
      TERPNET 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of biosynthetic gene disruption on triterpenoid content in Lotus japonicus2017

    • Author(s)
      Suzuki H, Fukushima EO, Seki H, Muranaka T
    • Organizer
      第35回日本植物細胞分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゲノム編集の原理と農芸化学・育種分野への可能性2017

    • Author(s)
      村中俊哉
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府・京都市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ゲノム編集による植物テルペノイド生合成のリデザイン2017

    • Author(s)
      村中俊哉、關光、福島エリオデット
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      東北大学(宮城県・仙台市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] The Arabidopsis SnRk1 (AKIN10) phosphorylates and down regulates AtHMGR1 activity2016

    • Author(s)
      Robertlee J, Kobayashi K, Suzuki M, Muranaka T
    • Organizer
      第29回植物脂質シンポジウム
    • Place of Presentation
      大阪大学(大阪府・豊中市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] トマトのトリテルペノイド生合成に関与する CYP716 family P450 酵素の解析2016

    • Author(s)
      安本周平、關光、清水裕子、福島エリオデット、村中俊哉
    • Organizer
      第29回植物脂質シンポジウム
    • Place of Presentation
      大阪大学(大阪府・豊中市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] テルペノイドの代謝多様性と遺伝子ディスカバリー2016

    • Author(s)
      關光、福島エリオデット、村中俊哉
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      ANAクラウンプラザホテル(富山県・富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 植物トリテルペノイドの生理機構解明に向けて2016

    • Author(s)
      村中俊哉、中園幹生
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ゲノム編集技術を用いたナス科作物の代謝改変ならびに育種素材開発に向けて2016

    • Author(s)
      村中俊哉
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2016-09-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 薬用植物カンゾウのトリテルペノイド生合成制御に関わる転写因子の探索2016

    • Author(s)
      田村啓太、(8名)、村中俊哉
    • Organizer
      第34回日本植物細胞分子生物学会(上田)大会
    • Place of Presentation
      信州大学(長野県・上田市)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] 高等植物由来CYP716Aサブファミリー酵素の酸化活性比較2016

    • Author(s)
      福島エリオデット、鈴木隼人、梅基直行、關光、村中俊哉
    • Organizer
      第34回日本植物細胞分子生物学会(上田)大会
    • Place of Presentation
      信州大学(長野県・上田市)
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] Engineering of plant triterpenoid biosynthesis with genome editing2016

    • Author(s)
      Muranaka T
    • Organizer
      13th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
    • Place of Presentation
      サイモン・フレーザー大学(カナダ・バンクーバー)
    • Year and Date
      2016-07-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Characterization of CYP716A subfamily genes in Arabidopsis thaliana2016

    • Author(s)
      Yasumoto S, Seki H, Fukushima EO, Muranaka T
    • Organizer
      13th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
    • Place of Presentation
      サイモン・フレーザー大学(カナダ・バンクーバー)
    • Year and Date
      2016-07-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Generation of cyp716a1 cyp716a2 double knockout Arabidopsis thaliana plants by TALEN technology2016

    • Author(s)
      Yasumoto S, Seki H, Fukushima EO, Muranaka T
    • Organizer
      Plant Genome Stability and Change 2016
    • Place of Presentation
      湘南国際村センター(神奈川・葉山市)
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高度機能分化した植物組織培養による有用サポニン生産技術開発2016

    • Author(s)
      村中俊哉
    • Organizer
      日本農芸化学会2016年度札幌大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] シロイヌナズナCYP716Aサブファミリー酵素遺伝子の機能解析2016

    • Author(s)
      安本周平、福島エリオデット、關光、村中俊哉
    • Organizer
      日本農芸化学会2016年度札幌大会
    • Place of Presentation
      札幌コンベンションセンター(北海道札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] ゲノム編集技術を用いた植物育種の現状と展望2016

    • Author(s)
      村中俊哉
    • Organizer
      日本農芸化学会関西支部 第497回講演会
    • Place of Presentation
      神戸大学(兵庫県・神戸市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ゲノム編集技術を用いた植物育種 その現状と展望2016

    • Author(s)
      村中俊哉
    • Organizer
      広島大学公開シンポジウム
    • Place of Presentation
      広島大学(広島県・東広島市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Terpenoid biosynthesis: Chemical diversity and their application to metabolic engineering2016

    • Author(s)
      MURANAKA T
    • Organizer
      KAIST-OU Symposium
    • Place of Presentation
      KAIST(韓国・大田広域市)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 新植物育種技術としてのゲノム編集2016

    • Author(s)
      村中俊哉
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜(神奈川県・横浜市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ゲノム編集の原理と育種への可能性2016

    • Author(s)
      村中俊哉
    • Organizer
      第3回日本生物工学会西日本支部講演会
    • Place of Presentation
      徳島大学(徳島県・徳島市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 毛状根培養を用いたゲノム編集のための人工ヌクレアーゼ評価系の構築2015

    • Author(s)
      安本周平, 澤井学, 若林孝俊, 關光, 刑部祐里子, 刑部敬史, 村中俊哉
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      城山観光ホテル(鹿児島県鹿児島市)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Triterpenoid biosynthesis: Diversity and their application to metabolic engineering2015

    • Author(s)
      Toshiya MURANAKA
    • Organizer
      TERPNET 2015
    • Place of Presentation
      ブリティッシュコロンビア大学(バンクーバー、カナダ)
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Production of plants triterpenoids in both transgenic plants and yeast culture system2015

    • Author(s)
      Toshiya MURANAKA
    • Organizer
      2nd SBJ Symposium
    • Place of Presentation
      大阪大学銀杏会館(大阪府吹田市)
    • Year and Date
      2015-05-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] 実験医学別冊、論文だけではわからないゲノム編集成功の秘訣Q6A2016

    • Author(s)
      山本卓(編)、村中俊哉、他
    • Total Pages
      268
    • Publisher
      羊土社
    • Related Report
      2016 Annual Research Report
  • [Book] ゲノム編集入門2016

    • Author(s)
      山本卓(編)、村中俊哉、他
    • Total Pages
      226
    • Publisher
      裳華房
    • Related Report
      2016 Annual Research Report
  • [Book] 進化するゲノム編集技術2015

    • Author(s)
      安本周平,關光,村中俊哉 他
    • Total Pages
      386
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2015 Annual Research Report
  • [Book] 実験医学別冊 論文だけではわからないゲノム編集成功の秘訣 Q&A2015

    • Author(s)
      安本周平,關光,刑部祐里子,刑部敬史,村中俊哉 他
    • Total Pages
      268
    • Publisher
      羊土社
    • Related Report
      2015 Annual Research Report
  • [Remarks] 村中研究室ホームページ

    • URL

      http://www.bio.eng.osaka-u.ac.jp/pl/index.html

    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 22α位がヒドロキシ基である五環系トリテルペン化合物の製造方法2016

    • Inventor(s)
      村中俊哉、関光、福島エリオデット、安本周平
    • Industrial Property Rights Holder
      村中俊哉、関光、福島エリオデット、安本周平
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-016511
    • Filing Date
      2016-01-29
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2021-01-27  

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