• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on structure and function of the processing enzyme for pre-tRNA and its application

Research Project

Project/Area Number 15H04487
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionKyushu University

Principal Investigator

Kimura Makoto  九州大学, 農学研究院, 学術特任教員 (10204992)

Co-Investigator(Kenkyū-buntansha) 中島 崇  九州大学, 農学研究院, 助教 (20380553)
角田 佳充  九州大学, (連合)農学研究科(研究院), 教授 (00314360)
Co-Investigator(Renkei-kenkyūsha) MAYANAGI Kota  九州大学, 生体防御医学研究所, 助教 (50418571)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
KeywordsリボヌクレアーゼP / アーキア / tRNAプロセシング酵素 / 超好熱性アーキア / リボヌクレアーゼ P / 透過型電子顕微鏡 / リボヌクレアーゼP / 前駆体tRNA / リボ核タンパク質 / RNA結合タンパク質 / 前駆体tRNAのプロセシング / リボ核タンパク質複合体酵素 / 生体触媒の分子進化
Outline of Final Research Achievements

Ribonuclease P (RNase P) is a ribonucleoprotein that cleaves off 5´ leader sequences from tRNA precursors in all living cells and consists of a catalytic RNA and protein cofactors. In this study, I performed the structure and functional study of RNase P from the hyperthermophilic archaeon Pyrococcus horioshii and also examined potentiality of the RNase P proteins for bioanalytical application where precise hybridization of nucleic acids is needed.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (10 results)

All 2018 2017 2016 2015

All Journal Article (7 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 7 results,  Open Access: 1 results,  Acknowledgement Compliant: 5 results) Presentation (2 results) Book (1 results)

  • [Journal Article] Crystal structure of an archaeal ribonuclease P protein Rpp38 in complex with RNA fragments containing a K-turn motif2018

    • Author(s)
      K. Oshima, X. Gao, S. Hayashi, T. Ueda, T. Nakashima, and M. Kimura
    • Journal Title

      Acta Cryst.

      Volume: F74 Issue: 1 Pages: 57-64

    • DOI

      10.1107/s2053230x17018039

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A three-dimensional model of RNase P of the hyperthermophilic archaeon Pyrococcus horikoshii OT32017

    • Author(s)
      X. Gao, K. Oshima, T. Ueda, T. Nakashima, and M. Kimura
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 493 Issue: 2 Pages: 1063-1068

    • DOI

      10.1016/j.bbrc.2017.09.085

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Functional characterization of archaeal homologues of a human nuclear RNase P proteins Rpp21 and Rpp29 provides insights into the molecular basis of their cooperativity in catalysis2017

    • Author(s)
      180.D. Jiang, K. Izumi, T. Ueda, K. Oshima, T. Nakashima, and M. Kimura
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 482 Issue: 1 Pages: 68-74

    • DOI

      10.1016/j.bbrc.2016.10.142

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Structural basis for recognition of a kink-turn motif by an archaeal homologue of human RNase P protein Rpp382016

    • Author(s)
      179.K. Oshima, Y. Kakiuchi, Y. Tanaka, T. Ueda, T. Nakashima, M. Kimura, and M. Yao
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 474 Issue: 3 Pages: 541-546

    • DOI

      10.1016/j.bbrc.2016.04.118

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Functional implication of the heteroterameric formation of archaeal homologues of human RNase P protein pairs Pop5 with Rpp302016

    • Author(s)
      M. Hamasaki, K. Hazeyama, F. Iwasaki, T. Ueda, T. Nakashima, Y. Kakuta, and M. Kimura
    • Journal Title

      J. Biochem

      Volume: 159 Pages: 31-40

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Archaeal ribonuclease P proteins are potential for biotechnological application where a precise hybridization of nucleic acids is needed2015

    • Author(s)
      M. Miyanoshita, T. Nakashima, Y. Kakuta and M. Kimura
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 79 Pages: 2014-2017

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Mutation of the gene encoding the ribonuclease P RNA in the hyperthermophilic archaeon Thermococcus kodakarensis causes decreased growth rate and impaired processing of tRNA precursors2015

    • Author(s)
      T. Ueda, S. Ishino, K. Suematsu, T. Nakashima, Y. Kakuta, Y. Ishino, and M. Kimura
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 468 Pages: 660-665

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 核酸結合タンパク質の構造機能相関と機能開発2017

    • Author(s)
      木村 誠
    • Organizer
      日本農芸化学会支部合同大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 超好熱性アーキアRNase P構成タンパク質のRNA活性化の構造基盤2015

    • Author(s)
      木村誠
    • Organizer
      日本生化学・分子生物学会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Book] アーキア生物学2017

    • Author(s)
      木村 誠 等
    • Total Pages
      201
    • Publisher
      共立出版
    • ISBN
      9784320057852
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi