| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
We identified two ergosteryl glucoside (EG) synthase genes of the pathogenic fungus Cryptococcus neoformans, and generated each gene-deficient mutant and both genes double-deficient mutants. Analyses of the defective mutants showed that EG synthesis is important for the heat stress tolerance of C. neoformans, therby it relates to the survival in the mouse and its pathogenecity for mouse. On the other hand, we found that marine eukaryotic microbe Aurantiochytrium limacinum synthesizes various steryl glucosides (SGs) in which glucose is bound to various sterols. Furthermore, we identified a novel acyl steryl glucoside in which DHA is bound to glucose of SG in A. limacinum.
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