Elucidation of a transmitting mechanism of the information of odorants and pheromones, based on the fMRI analysis of the olfactory bulb and the higher-order brain regions and the behavior analysis

• Project/Area Number: 15H04489
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kumamoto University
• Principal Investigator: Terasawa Hiroaki 熊本大学, 大学院生命科学研究部(薬), 教授 (10300956)
• Co-Investigator(Renkei-kenkyūsha): YOSHINAGA SOSUKE 熊本大学, 大学院生命科学研究部(薬), 講師 (00448515) ; TOUHARA Kazushige 東京大学, 大学院農学生命科学研究科, 教授 (00280925)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
• Keywords: 情報伝達 / 匂い / フェロモン / MRI / ＭＲＩ

Outline of Final Research Achievements

For the BOLD (Blood Oxygenation Level-Dependent)-based functional MRI analyses, a technique to extract the MR signal changes that synchronize periodical and repetitive olfactory stimulations was applied. As a result, we succeeded in higher sensitive detection of the mouse brain regions responding to each odorant or pheromone stimulation. We also developed an automatic olfactory stimulation apparatus to handle many kinds of odorants and pheromones. The fMRI development and optimization of the measuring and analytical techniques and the apparatus during this project paved the way to comprehensively analyze responses to odorants and pheromones with higher sensitivity.

Research Products

• [Journal Article] A BOLD analysis of the olfactory perception system in the mouse whole brain, using independent component analysis. (2017)
  • Author(s): Funatsu, H., Hayashi, F., Yoshinaga, S., Takeda, M., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 25 Pages: 5363-5363
  • Peer Reviewed
• [Journal Article] An optimized slice-GRAPPA reconstruction method to reduce leakage artifacts in small-animal multiband imaging. (2017)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Takeda, M., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 25 Pages: 3844-3844
  • Peer Reviewed
• [Journal Article] Development of an awake mouse MRI method using soft immobilization for a cryogenic probe system. (2016)
  • Author(s): Nakata, E., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., Yoshinaga, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 24 Pages: 1757-1757
  • Peer Reviewed
• [Journal Article] An iterative reconstruction method for dual-band EPI in small-animal studies. (2016)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 24 Pages: 3242-3242
  • Peer Reviewed
• [Journal Article] Image-based phase correction for dual-band EPI with slice-GRAPPA using point-by-point procedures in k-space. (2016)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 24 Pages: 4315-4315
  • Peer Reviewed
• [Journal Article] MEMRI and BOLD analyses of the olfactory perception system in response to odorant stimuli in mice. (2015)
  • Author(s): Funatsu, H., Yoshinaga, S., Goto, H., Hirakane, M., Iwamoto, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 23 Pages: 2033-2033
  • Peer Reviewed
• [Journal Article] Pre-scan with half-sized phase encoding blips reducing ghost and slice leakage artifacts in dual-band EPI. (2015)
  • Author(s): Toyoda, H., Yuzuriha, N., Yoshinaga, S., and Terasawa, H.
  • Journal Title: Proc. Intl. Soc. Mag. Reson. Med. Volume: 23 Pages: 2406-2406
  • Peer Reviewed
• [Journal Article] A novel reconstruction method for multi-band EPI in animal scanners with iterative point-by-point procedures in k-space. (2015)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Journal Title: Magn. Reson. Mater. Phys. Biol. Med. Volume: 28
  • Peer Reviewed
• [Presentation] BOLD法を用いた異なる匂い刺激および麻酔下におけるマウス高次脳の嗅覚応答の比較 (2018)
  • Author(s): 林 芙優、吉永 壮佐、杠 直哉、武田 光広、寺沢 宏明
  • Organizer: ISMRM日本支部第２回学術集会
• [Presentation] 独立成分解析を利用したマウス嗅覚受容システムの BOLD 解析 (2017)
  • Author(s): 舩津大嗣、林芙優、吉永壮佐、杠直哉、草薙俊輔、武田光広、寺沢宏明
  • Organizer: ISMRM日本支部第１回学術集会
  • Place of Presentation: 脳情報通信融合研究センター、大阪
  • Year and Date: 2017-02-23
• [Presentation] A BOLD analysis of the olfactory perception system in the mouse whole brain, using independent component analysis. (2017)
  • Author(s): Funatsu, H., Hayashi, F., Yoshinaga, S., Takeda, M., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Organizer: 25th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Int'l Joint Research
• [Presentation] An optimized slice-GRAPPA reconstruction method to reduce leakage artifacts in small-animal multiband imaging. (2017)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Takeda, M., and Terasawa, H.
  • Organizer: 25th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Int'l Joint Research
• [Presentation] Detection of the odor response in the mouse whole brain using periodic odor stimulations and independent component analysis (ICA). (2017)
  • Author(s): Hayashi, F., Funatsu, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., Takeda, M., and Terasawa, H.
  • Organizer: 第４５回日本磁気共鳴医学会大会
• [Presentation] Improvement of an awake mouse MRI method using mouse clothes for a cryogenic coil system. (2017)
  • Author(s): Nakata, E., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., Yoshinaga, S., and Terasawa, H.
  • Organizer: 第４５回日本磁気共鳴医学会大会
• [Presentation] Detection of the odor BOLD response in the mouse whole brain, using independent component analysis (ICA). (2017)
  • Author(s): Funatsu, H., Hayashi, F., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., Takeda, M., and Terasawa, H.
  • Organizer: 第５５回日本生物物理学会年会
• [Presentation] Evaluation of anesthesia conditions for detecting odor responses in the mouse whole brain. (2017)
  • Author(s): Hayashi, F., Funatsu, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., Takeda, M., and Terasawa, H.
  • Organizer: 第５５回日本生物物理学会年会
• [Presentation] Optimization of the awake mouse MRI method using mouse clothes for a cryogenic coil system. (2016)
  • Author(s): Nakata, E., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., Yoshinaga, S., and Terasawa, H.
  • Organizer: 第４４回日本磁気共鳴医学会大会
  • Place of Presentation: 大宮ソニックシティ、埼玉
  • Year and Date: 2016-09-10
• [Presentation] Detecting the olfactory responses in the mouse whole brain using a cryogenic probe. (2016)
  • Author(s): Funatsu, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., Takeda, M., and Terasawa, H.
  • Organizer: 第４４回日本磁気共鳴医学会大会
  • Place of Presentation: 大宮ソニックシティ、埼玉
  • Year and Date: 2016-09-10
• [Presentation] fMRI analyses of the mouse olfactory perception system using a cryogenic probe. (2016)
  • Author(s): Funatsu, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., Takeda, M., and Terasawa, H.
  • Organizer: The XXVIIth ICMRBS (International Conference on Magnetic Resonance in Biological Systems)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Presentation] Development of an awake mouse MRI method using soft immobilization for a cryogenic probe system. (2016)
  • Author(s): Nakata, E., Kusanagi, S., Kimura, K., Araki, R., Takeda, M., Yoshinaga, S., and Terasawa, H.
  • Organizer: 24th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Suntec Singapore Convention & Exhibition Centre, Singapore
  • Year and Date: 2016-05-09
  • Int'l Joint Research
• [Presentation] Image-based phase correction for dual-band EPI with slice-GRAPPA using point-by-point procedures in k-space. (2016)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., and Terasawa, H.
  • Organizer: 24th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Suntec Singapore Convention & Exhibition Centre, Singapore
  • Year and Date: 2016-05-09
  • Int'l Joint Research
• [Presentation] An iterative reconstruction method for dual-band EPI in small-animal studies. (2016)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Organizer: 24th ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Suntec Singapore Convention & Exhibition Centre, Singapore
  • Year and Date: 2016-05-09
  • Int'l Joint Research
• [Presentation] BOLD法を用いた匂い刺激によるマウス脳活性化の検出 (2015)
  • Author(s): 舩津大嗣、吉永壮佐、平金真、岩本成人、寺沢宏明
  • Organizer: 第５４回ＮＭＲ討論会
  • Place of Presentation: 千葉工業大学津田沼キャンパス、千葉
  • Year and Date: 2015-11-08
• [Presentation] A novel reconstruction method for multi-band EPI in animal scanners with iterative point-by-point procedures in k-space. (2015)
  • Author(s): Toyoda, H., Yoshinaga, S., Yuzuriha, N., Kusanagi, S., and Terasawa, H.
  • Organizer: ESMRMB (European Society for Magnetic Resonance in Medicine and Biology) 2015 32nd annual scientific meeting
  • Place of Presentation: Edinburgh International Conference Centre (EICC), Edinburgh, U.K.
  • Year and Date: 2015-10-01
  • Int'l Joint Research
• [Presentation] 極低温プローブに適用可能なマウス服を用いたawake MRI測定法の開発 (2015)
  • Author(s): 草薙俊輔、木村一成、平金真、岩本成人、荒木力太、吉永壮佐、寺沢宏明
  • Organizer: 第４３回日本磁気共鳴医学会大会
  • Place of Presentation: 東京ドームホテル、東京
  • Year and Date: 2015-09-11
• [Presentation] BOLD 法を用いた匂い刺激によるマウス嗅球の活性化の検出 (2015)
  • Author(s): 舩津大嗣、吉永壮佐、平金真、岩本成人、寺沢宏明
  • Organizer: 第４３回日本磁気共鳴医学会大会
  • Place of Presentation: 東京ドームホテル、東京
  • Year and Date: 2015-09-10
• [Presentation] Development of an awake mouse MR imaging method using soft immobilization for a cryogenic probe system. (2015)
  • Author(s): Kusanagi, S., Kimura, K., Funatsu, H., Hirakane, M., Iwamoto, S., Araki, R., Yoshinaga, S., and Terasawa, H.
  • Organizer: WMIC (World Molecular Imaging Congress) 2015
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, U.S.A.
  • Year and Date: 2015-09-02
  • Int'l Joint Research
• [Presentation] A robust Nyquist ghost suppression for high-resolution echo planar imaging of small animal brains. (2015)
  • Author(s): Toyoda, H., Yuzuriha, N., Yoshinaga, S., and Terasawa, H.
  • Organizer: OHBM (Organization for Human Brain Mapping) annual conference 2015
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, U.S.A.
  • Year and Date: 2015-06-14
  • Int'l Joint Research
• [Presentation] MEMRI and BOLD analyses of the olfactory perception system in response to odorant stimuli in mice. (2015)
  • Author(s): Funatsu, H., Yoshinaga, S., Goto, H., Hirakane, M., Iwamoto, S., and Terasawa, H.
  • Organizer: 23rd ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Metro Toronto Convention Centre, Toronto, Canada
  • Year and Date: 2015-06-01
  • Int'l Joint Research
• [Presentation] Pre-scan with half-sized phase encoding blips reducing ghost and slice leakage artifacts in dual-band EPI. (2015)
  • Author(s): Toyoda, H., Yuzuriha, N., Yoshinaga, S., and Terasawa, H.
  • Organizer: 23rd ISMRM (International Society for Magnetic Resonance in Medicine) annual meeting
  • Place of Presentation: Metro Toronto Convention Centre, Toronto, Canada
  • Year and Date: 2015-06-01
  • Int'l Joint Research