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Identification of ancestral gibberellin in a moss

Research Project

Project/Area Number 15H04492
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

NAKAJIMA MASATOSHI  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (50237278)

Co-Investigator(Kenkyū-buntansha) 川出 洋  東京農工大学, (連合)農学研究科(研究院), 教授 (20291916)
Co-Investigator(Renkei-kenkyūsha) HAYASHI Ken-ichiro  岡山理科大学, 理学部, 教授 (30289136)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywordsコケ植物 / 分化誘導物質 / 生理活性物質 / ジベレリン / ジテルペン / 植物 / ホルモン / コケ / 分化制御
Outline of Final Research Achievements

The moss Physcomitrella patens has a partial biosynthetic pathway for gibberellin and produces an intermediate, ent-kaurenoic acid (KA). Protonemal cell differentiation is incomplete under KA-deficiency and restored by KA application but not by gibberellin. Herein, two KA-metabolites, ent-3β-hydroxy-kaurenoic acid and ent-2α-hydroxy-kaurenoic acid were identified. The oxidation of KA at the C-3/C-2 position determines the activation or inactivation of the KA-metabolism, which appears to be an ancient type of gibberellin regulation.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (27 results)

All 2018 2017 2016 2015

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (20 results) (of which Int'l Joint Research: 10 results,  Invited: 1 results)

  • [Journal Article] Characterization of moss ent-kaurene oxidase (CYP701B1) using a highly purified preparation2018

    • Author(s)
      Chisato Noguchi, Sho Miyazaki, Hiroshi Kawaide, Osamu Gotoh, Yuzo Yoshida and Yuri Aoyama
    • Journal Title

      Journal of Biochemistry

      Volume: 163 Issue: 1 Pages: 69-76

    • DOI

      10.1093/jb/mvx063

    • NAID

      40021457423

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In planta functions of cytochrome P450 monooxygenase genes in the phytocassane biosynthetic gene cluster on rice chromosome 22018

    • Author(s)
      Zhongfeng Ye, Kohei Yamazaki, Hiromi Minoda, Koji Miyamoto, Sho Miyazaki, Hiroshi Kawaide, Arata Yajima, Hideaki Nojiri, Hisakazu Yamane and Kazunori Okada
    • Journal Title

      Bioscience, Biotechnology and Biochemistry

      Volume: 82 Issue: 6 Pages: 1021

    • DOI

      10.1080/09168451.2017.1398067

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An ancestral gibberellin in a moss Physcomitrella patens2018

    • Author(s)
      Sho Miyazaki, Mariho Hara, Shinsaku Ito, Keisuke Tanaka, Tadao Asami, Ken-ichiro Hayashi, Hiroshi Kawaide and Masatoshi Nakajima
    • Journal Title

      Molecular Plant

      Volume: 印刷中 Issue: 8 Pages: 1097-1100

    • DOI

      10.1016/j.molp.2018.03.010

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Assays of Protonemal Growth Responses in Physcomitrella patens under Blue- and Red-light Stimuli2018

    • Author(s)
      Sho Miyazaki, Masatoshi Nakajima and Hiroshi Kawaide
    • Journal Title

      Methods in Molecular Biology

      Volume: (nknown)

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Helminthosporic acid functions as an agonist for gibberellin receptor2017

    • Author(s)
      Sho Miyazaki, Jiang Kai, Masatomo Kobayashi, Tadao Asami and Masatoshi Nakajima
    • Journal Title

      Bioscience, Biotechnology and Biochemistry

      Volume: 81 Issue: 11 Pages: 2152-2159

    • DOI

      10.1080/09168451.2017.1381018

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HpDTC1, a Stress-Inducible Bifunctional Diterpene Cyclase Involved in Momilactone Biosynthesis, Functions in Chemical Defence in the Moss Hypnum plumaeforme2016

    • Author(s)
      Kazunori Okada, Hiroshi Kawaide, Koji Miyamoto, Sho Miyazaki, Ryousuke Kainuma, Honoka Kimura, Kaoru Fujiwara, Masatoshi Nakajima, Hisakazu Yamane, Yuki Hatano, Hiroshi Nozaki, Ken-ichiro Hayashi
    • Journal Title

      SCIENTIFIC REPORTS

      Volume: 6 Issue: 1 Pages: 1-12

    • DOI

      10.1038/srep25316

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Analysis of ent-kaurenoic acid by ultra performance liquid chromatography-tandem mass spectrometry.2015

    • Author(s)
      S. Miyazaki, H. Kimura, M. Natsume, T. Asami, K. Hayashi, H. Kawaide, and M. Nakajima
    • Journal Title

      Biochem. Biophys. Reports

      Volume: 2 Pages: 103-107

    • DOI

      10.1016/j.bbrep.2015.05.010

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 陸上植物における生理活性ジテルペノイドの生合成にみられる機能進化2018

    • Author(s)
      川出 洋, 宮崎 翔, 林 謙一郎, 岡田 憲典, 中嶋 正敏
    • Organizer
      農芸化学会2018年度大会, 名古屋
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 下等植物から高等植物に至るent-kaurene合成酵素の機能進化2018

    • Author(s)
      福田 和佳子, 宮崎 翔, 牧野 晴香, 木村 穂乃香, 貝沼 遼介, 安藤 朋子, 夏目 雅裕,野崎 浩, 林 謙一郎, 川出 洋
    • Organizer
      農芸化学会2018年度大会, 名古屋
    • Related Report
      2017 Annual Research Report
  • [Presentation] 蘚類におけるジャスモン酸類の探索と生理活性の追究2018

    • Author(s)
      照屋 美優、藤原 薫、宮本 皓司、山根 久和、新屋 友規、ガリス イバン、林 謙一郎、宮崎 翔、中嶋 正敏、野尻 秀昭、岡田 憲典
    • Organizer
      農芸化学会2018年度大会, 名古屋
    • Related Report
      2017 Annual Research Report
  • [Presentation] 原始ジベレリン様成長制御物質の同定2018

    • Author(s)
      宮崎 翔, 原 万里穂, 伊藤 晋作, 田中 啓介, 川出 洋, 林 謙一郎, 浅見 忠男, 中嶋 正敏
    • Organizer
      農芸化学会2018年度大会, 名古屋
    • Related Report
      2017 Annual Research Report
  • [Presentation] An ancestral gibberellin biosynthetic pathway in the moss2017

    • Author(s)
      Sho Miyazaki, Mariho Hara, Shinsaku Ito, Keisuke Tanaka, Tadao Asami, Ken-ichiro Hayashi, Hiroshi Kawaide, Masatoshi Nakajima
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ハイゴケ由来の相同性の高い二つのジテルペン環化酵素の構造と機能における進化的関係2017

    • Author(s)
      福田 和佳子, 宮崎 翔, 牧野 晴香, 木村 穂乃香, 貝沼 遼介, 安藤 朋子, 夏目 雅裕,野崎 浩, 林 謙一郎, 川出 洋
    • Organizer
      植物化学調節学会第52回大会, 鹿児島
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒメツリガネゴケの原糸体の分化を促す化合物の活性評価2017

    • Author(s)
      小野 真太郎, 梁 彩東, 宮崎 翔, 林 謙一郎, 川出 洋, 浅見 忠男, 中嶋 正敏
    • Organizer
      植物化学調節学会第52回大会, 鹿児島
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒメツリガネゴケ原糸体の分化制御に関わる新奇生理活性物質の同定2017

    • Author(s)
      宮崎 翔, 原 万里穂, 伊藤 晋作, 田中 啓介, 川出 洋, 林 謙一郎, 浅見 忠男, 中嶋 正敏
    • Organizer
      植物化学調節学会第52回大会, 鹿児島
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒメツリガネゴケ原糸体の分化制御に関わる新奇生理活性物質の同定2017

    • Author(s)
      宮崎 翔, 原 万里穂, 伊藤 晋作, 田中 啓介, 川出 洋, 林 謙一郎, 浅見 忠男, 中嶋 正敏
    • Organizer
      日本農芸化学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Identification of novel diterpene-type growth regulator in Physcomitrella patens2016

    • Author(s)
      Sho Miyazaki, Hiroshi Kawaide, Ken-ichiro Hayashi, Tadao Asami and Masatoshi Nakajima
    • Organizer
      MOSS リーズ(イギリス)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒメツリガネゴケの生活環で機能するジベレリン様成長制御物質の探索2016

    • Author(s)
      宮崎 翔, 原 万里穂, Seung-Hyun Park, 川出 洋, 林 謙一郎, 浅見 忠男, 中嶋 正敏
    • Organizer
      日本蘚苔類学会 (鹿児島)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Endogenous ent-kaurenoic acid-metabolite regulates the differentiation of Physcomitrella patens2016

    • Author(s)
      Sho Miyazaki, Mariho Hara, Hiroshi Kawaide, Ken-ichiro Hayashi, Tadao Asami and Masatoshi Nakajima
    • Organizer
      IPGSA トロント(カナダ)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A moss-specific diterpenoid hormone regulates protonemal growth and development2016

    • Author(s)
      Hiroshi Kawaide, Sho Miyazaki and Masatoshi Nakajima
    • Organizer
      IPGSA トロント(カナダ)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Screening of chemicals that affect protonemal differentiation of a moss Physcomitrella patens2016

    • Author(s)
      Masatoshi Nakajima, Yang Che-Dong, Sho Miyazaki, Seung-Hyun Park, Masato Otani, Hiroshi Kawaide, Ken-ichiro Hayashi and Tadao Asami
    • Organizer
      IPGSA トロント(カナダ)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Screening of chemicals that affect protonemal differentiation of Physcomitrella patens2015

    • Author(s)
      Masatoshi Nakajima, Che-Dong Yang, Sho Miyazaki, Seung-Hyun Park, Masato Otani, Hiroshi Kawaide, Ken-ichiro Hayashi, Tadao Asami
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      米国・ホノルル
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Hormonal diterpenoids besides gibberellin are involved in photomorphogenesis of Physcomitrella patens2015

    • Author(s)
      Sho Miyazaki, Seung-Hyun Park, Hiroshi Kawaide, Ken-ichiro Hayashi, Tadao Asami, Masatoshi Nakajima
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      米国・ホノルル
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biosynthesis and physiological function of a moss-specific diterpenoid hormone2015

    • Author(s)
      Hiroshi Kawaide, Sho Miyazaki, Masatoshi Nakajima
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      米国・ホノルル
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒメツリガネゴケ原糸体の分化に関与するジベレリン様成長制御物質の解明2015

    • Author(s)
      宮崎 翔, Che-Dong Yang, Seung-Hyun Park, 川出 洋, 林 謙一郎, 浅見忠男, 中嶋正敏
    • Organizer
      植物化学調節学会第50回大会
    • Place of Presentation
      東京大学農学部(東京都文京区)
    • Year and Date
      2015-10-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Hormonal diterpenoids are involved in photomorphogenesis under the blue-light in Physcomitrella patens2015

    • Author(s)
      Sho Miyazaki, Hiroshi Kawaide and Masatoshi Nakajima
    • Organizer
      TERPNET 2015
    • Place of Presentation
      カナダ・バンクーバー
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional evolution of ent-kaurene synthases of bryophytes and beyond2015

    • Author(s)
      Hiroshi Kawaide, Sho Miyazaki, Masatoshi Nakajima
    • Organizer
      TERPNET 2015
    • Place of Presentation
      カナダ・バンクーバー
    • Year and Date
      2015-06-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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