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Imaging analysis of plant growth regulator using radioisotope imaging technique

Research Project

Project/Area Number 15H04493
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

Tanoi Keitaro  東京大学, 大学院農学生命科学研究科(農学部), 教授 (90361576)

Co-Investigator(Kenkyū-buntansha) 矢崎 一史  京都大学, 生存圏研究所, 教授 (00191099)
高梨 功次郎  信州大学, 先鋭領域融合研究群山岳科学研究所, 助教(特定雇用) (10632119)
小林 奈通子  東京大学, 大学院農学生命科学研究科(農学部), 助教 (60708345)
杉田 亮平  東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (60724747)
廣瀬 農  東京大学, 大学院農学生命科学研究科(農学部), 特任講師 (90708372)
Co-Investigator(Renkei-kenkyūsha) ASAMI Tadao  東京大学, 大学院農学生命科学研究科, 教授 (90231901)
NAKAMURA Hidemitsu  東京大学, 大学院農学生命科学研究科, 助教 (40724191)
OKADA Kazunori  東京大学, 生物生産工学研究センター, 准教授 (20312241)
NAKANISHI Tomoko  東京大学, 大学院農学生命科学研究科, 特任教授 (30124275)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywordsアイソトープイメージング / 植物生長調節物質 / 生理活性物質イメージング
Outline of Final Research Achievements

We applied two types of radioisotope imaging technique, live imaging technique and high resolution visualization technique to investigate auxin distribution in plant. As a result, our live imaging technique visualized 14C-auxin moving from the upper part of the plant to the root tip. The high resolution visualization technique visualized 14C-auxin at vascular bundle and vascular bundle organization. The present study showed that the radioisotope imaging techniques are able to obtain auxin distribute information.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (15 results)

All 2018 2017 2016 2015

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 1 results,  Open Access: 5 results) Presentation (8 results) (of which Invited: 1 results)

  • [Journal Article] T-DNA Tagging-Based Gain-of-Function of OsHKT1;4 Reinforces Na Exclusion from Leaves and Stems but Triggers Na Toxicity in Roots of Rice Under Salt Stress2018

    • Author(s)
      Yuuka Oda, Natsuko I. Kobayashi, Keitaro Tanoi, Jian Feng Ma, Yukiko Itou, Maki Katsuhara, Takashi Itou. Tomoaki Horie
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 19 Issue: 1 Pages: 235-235

    • DOI

      10.3390/ijms19010235

    • NAID

      120007099652

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of different Cs concentrations on overall plant growth and Cs distribution in soybean2018

    • Author(s)
      Nihei, N.; Tanoi, K.; Nakanishi, T. M
    • Journal Title

      Plant Production Science

      Volume: 21 Issue: 1 Pages: 26-31

    • DOI

      10.1080/1343943x.2018.1429219

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of how light changes affect ion movement in rice plants using a real-time radioisotope imaging system2017

    • Author(s)
      Sugita, R., Kobayashi, N.I., Hirose, Iwata, R., Suzuki, H., Tanoi, K., and Nakanishi, T.M.
    • Journal Title

      Journal of Radioanalytical and Nuclear Chemistry

      Volume: 印刷中 Issue: 3 Pages: 717-723

    • DOI

      10.1007/s10967-017-5193-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Acetate-mediated novel survival strategy against drought in plants2017

    • Author(s)
      Kim JM, To TK, Matsui A, Tanoi K, Kobayashi NI, Matsuda F, Habu Y, Ogawa D, Sakamoto T, Matsunaga S, Bashir K, Rasheed S, Ando M, Takeda H, Kawaura K, Kusano M, Fukushima A, Endo TA, Kuromori T, Ishida J, Morosawa T, Tanaka M, Torii C, Takebayashi Y, Sakakibara H, Ogihara Y, Saito K, Shinozaki K, Devoto A, Seki M
    • Journal Title

      Nature Plants

      Volume: 3 Issue: 7 Pages: 17097-17097

    • DOI

      10.1038/nplants.2017.97

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] OsHKT1;5 mediates Na+ exclusion in the vasculature to protect leaf blades and reproductive tissues from salt toxicity in rice2017

    • Author(s)
      Kobayashi Natsuko I.、Yamaji Naoki、Yamamoto Hiroki、Okubo Kaoru、Ueno Hiroki、Costa Alex、Tanoi Keitaro、Matsumura Hideo、Fujii-Kashino Miho、Horiuchi Tomoki、Nayef Mohammad Al、Shabala Sergey、An Gynheung、Ma Jian Feng、Horie Tomoaki
    • Journal Title

      The Plant Journal

      Volume: 91 Issue: 4 Pages: 657-670

    • DOI

      10.1111/tpj.13595

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Concentration Distributions of Cs in Soybean Seeds2017

    • Author(s)
      Nihei, N.; Sugiyama, A.; Ito, Y.; Onji, T.; Kita, K.; Hirose, A.; Tanoi, K.; Nakanishi, T. M
    • Journal Title

      RADIOISOTOPES

      Volume: 66 Issue: 7 Pages: 235-242

    • DOI

      10.3769/radioisotopes.66.235

    • NAID

      130005773748

    • ISSN
      0033-8303, 1884-4111
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fine-tuning of auxin homeostasis governs the transition from floral stem cell maintenance to gynoecium formation.2017

    • Author(s)
      Yamaguchi, N., Huang, J., Xu, Y., Tanoi, K., and Ito, T.
    • Journal Title

      Nature Communications

      Volume: 8 Issue: 1 Pages: 1125-1125

    • DOI

      10.1038/s41467-017-01252-6

    • NAID

      120006364326

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] イメージングプレートを用いた植物体内における14C、32Pのダブルトレーサ法の開発2017

    • Author(s)
      小川隆宏; 杉田亮平; 中西友子; 田野井慶太朗
    • Organizer
      第53回アイソトープ研究発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 放射性トレーサーを用いた植物における物質分布の可視化技術の開発2017

    • Author(s)
      田野井慶太朗
    • Organizer
      第12回トランスポーター研究会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Radioactive Caesium distribution and movement revealed by RI imaging technique we are devewloping2017

    • Author(s)
      Nakanishi, T.M.; Sugita, R.; Kobayashi, N.I.; Nihei, N.; Hirose, A.; Tanoi, K
    • Organizer
      IOCBTE 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Radiocaesium translocation to rice grain under different potassium condition2017

    • Author(s)
      Tanoi, K.; Shiomi, S.; Nobori, T.; Takagi, H.; Fukuda, Y.; Kobayashi, N.I.; Nakanishi, T.M.
    • Organizer
      IOCBTE 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Novel function of potassium and cesium channel, SKOR, involved in dormancy-induced nutrient re-allocation in poplar2017

    • Author(s)
      Furukawa, J.; Noda, Y.; Nihei, N.; Hirose, A.; Tanoi, K.; Satoh, S
    • Organizer
      IPNC 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Live imaging of ion movement in plants by Real-Time Radioisotope Imaging System (RRIS)2017

    • Author(s)
      Sugita, R.; Kobayashi, N.I.; Hirose, A.; Tanoi, K.; Nakanishi, T.M.
    • Organizer
      IPNC 2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] 凍結切片法による植物根における45Ca、35Sの分布解析2016

    • Author(s)
      木下慶生; 根岸辰成; 廣瀬 農; 小林奈通子; 中西友子; 田野井慶太朗
    • Organizer
      第53回アイソトープ・放射線研究発表会
    • Place of Presentation
      東京大学弥生講堂(東京都 文京区)
    • Year and Date
      2016-07-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] 凍結切片作製による植物根端の3H標識オーキシン分布の可視化2015

    • Author(s)
      根岸 辰成; 廣瀬 農; 田野井 慶太朗; 中西 友子
    • Organizer
      第52回アイソトープ・放射線研究発表会
    • Place of Presentation
      東京大学弥生講堂(東京都 文京区)
    • Year and Date
      2015-07-09
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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