Anti-inflammatory mechanism from small intestine to whole body via interferon-beta
| Project/Area Number |
15H04504
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Tsuji Noriko 国立研究開発法人産業技術総合研究所, 生命工学領域, 上級主任研究員 (30343990)
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| Co-Investigator(Kenkyū-buntansha) |
平山 和宏 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (60208858)
角田 茂 東京大学, 医科学研究所, 助教 (80345032)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Keywords | lactic acid bacteria / intreferon-beta / small intestine / anti-inflammation / dendritic cell / Peyer's patch / Th1 / oral tolerance / 腸管免疫 / 乳酸菌 / 樹状細胞 / トル様レセプター３ (TLR３) / インターフェロン-β / インターロイキン-12 / 抗炎症 / 食品機能 / インターフェロンβ / 小腸常在菌 / トル様レセプター3: TLR3 / トル様レセプター３: TLR３) |
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| Outline of Final Research Achievements |
Double-stranded RNA of lactic acid bacteria (LAB) is recognized by dendritic cells (DCs) via endosomal-TLR3 and benefits the anti-inflammatory response through induction of interferon-β (IFN-β). However, how such IFN-β impacts T cell immune responses, and how immune homeostasis is better maintained in the presence of commensal or food-derived LAB was unknown. Here we show that LAB enhances interleukin-12 (IL-12) secretion by DCs and differentiation of IFN-γ-producing T cells in an IFN-β-dependent manner. We demonstrated that IFN-β secreted in response to LAB increased IFN regulatory factor 1 (IRF1) and IRF7 mRNA, which contribute to Il12p35 expression. We clarified the DC subset in Peyer’s patches that induce Th1 cell differentiation through IFN-β production in response to LAB. Th1 polarization and maintenance of Foxp3 expression by CD4+ T cells due to TLR3-mediated IFN-β production may thus confer anti-allergic or anti-inflammatory activity by commensal or probiotic LAB.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Visualization of Probiotic-Mediated Ca2+ Signaling in Intestinal Epithelial Cells In Vivo.2016
Author(s)
*Adachi T, Kakuta S, Aihara Y, Kamiya T, Watanabe Y, Osakabe N, Hazato N, Miyawaki A, Yoshikawa S, Usami T, Karasuyama H, Kimoto-Nira H, *Hirayama K, *Tsuji NM.
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Journal Title
Front Immunol.
Volume: 7
Pages: 601-601
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] CRTAM determines the CD4+ cytotoxic T lymphocyte lineage.2016
Author(s)
Takeuchi, A., Badr Mel, S., Miyauchi, K., Ishihara, C., Onishi, R., Guo, Z., Sasaki. Y., Ike, H., Takumi, A., Tsuji, NM., Murakami, Y., Katakai, T., Kubo, M. and Saito, T.
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Journal Title
J. Exp. Med.
Volume: 213
Issue: 1
Pages: 123-138
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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