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Carbon fixation process into the tree using 13C labeling and NanoSIMS

Research Project

Project/Area Number 15H04513
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Forest science
Research InstitutionKyoto University

Principal Investigator

Dannoura Masako  京都大学, 地球環境学堂, 助教 (90444570)

Co-Investigator(Kenkyū-buntansha) 高橋 けんし  京都大学, 生存圏研究所, 准教授 (10303596)
竹内 美由紀  東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (20378912)
小南 裕志  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (70353688)
高梨 聡  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (90423011)
Project Period (FY) 2015-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywords気候変動・炭素収支 / 森林炭素循環 / パルスラベリング / 安定同位体 / イオン顕微鏡 / 炭素収支
Outline of Final Research Achievements

In this study, 13C pulse labeling was performed on several tree species in order to clarify the time and amount of CO2 was released after CO2 was fixed by trees. (1) 13CO2 in respiration was chased using Tunable Diode Laser Spectroscopy(TDLS),(2)13C in organic matter was chased using Isotope Ratio Mass Spectrometer (IRMS), and (3) distribution of 13C in what ?? was traced using an ion microscope, Nano Secondary Ion Mass Spectometry (NanoSIMS). It was possible to purify the carbon in bulk organic matter and follow the detailed variations in the soluble organic carbon and starch. By fractionating soluble organic carbon into sugars, we were able to obtain new knowledge about carbon allocation under dry conditions. It was difficult to observe 13C distribution in soluble carbon but could observed in starch granules by using NanoSIMS.

Academic Significance and Societal Importance of the Research Achievements

気候変動により地球上の様々な地域で乾燥が進むことが懸念されている。本研究により、樹木が乾燥条件下では光合成産物を単糖に変換して浸透圧を調整していることや、師部の形状を変化させていることが示され、乾燥条件下での樹木の炭素配分の一端を解明することができた。また樹種により、固定された炭素の、糖やデンプンなどの非構造性炭素への配分が異なることが明らかになった。このことは、環境応答を考える際に、樹種による違いを考慮に入れることが重要であることを示している。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (44 results)

All 2020 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (4 results) Journal Article (10 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 10 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (29 results) (of which Int'l Joint Research: 10 results,  Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] INRA/Universite de Nancy(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] INRA-Nancy/Universite de Lorraine/INRA-Bordeaux(フランス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] INRA-Nancy/Universite de Lorraine/INRA-Bordeaux(フランス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Universite de Lorraine/INRA(フランス)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 炭素収支における<sup>13</sup>Cパルスラベリング手法の適用と展望2019

    • Author(s)
      檀浦正子
    • Journal Title

      Shinrin Kagaku

      Volume: 85 Issue: 0 Pages: 13-18

    • DOI

      10.11519/jjsk.85.0_13

    • NAID

      130007610895

    • ISSN
      0917-1908, 2424-1490
    • Year and Date
      2019-02-01
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Introduction to the invited issue on phloem function and dysfunction2019

    • Author(s)
      Epron Daniel、Dannoura Masako、Holtta Teemu
    • Journal Title

      Tree Physiology

      Volume: 39 Issue: 2 Pages: 167-172

    • DOI

      10.1093/treephys/tpz007

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Drought impacts on tree phloem: from cell-level responses to ecological significance2019

    • Author(s)
      Salmon Yann、Dietrich Lars、Sevanto Sanna、Holtta Teemu、Dannoura Masako、Epron Daniel
    • Journal Title

      Tree Physiology

      Volume: 39 Issue: 2 Pages: 173-191

    • DOI

      10.1093/treephys/tpy153

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Initial hydraulic failure followed by late-stage carbon starvation leads to drought-induced death in the tree Trema orientalis2019

    • Author(s)
      Kono Yuri、Ishida Atsushi、Saiki Shin-Taro、Yoshimura Kenichi、Dannoura Masako、Yazaki Kenichi、Kimura Fuku、Yoshimura Jin、Aikawa Shin-ichi
    • Journal Title

      Communications Biology

      Volume: 2 Issue: 1 Pages: 8-16

    • DOI

      10.1038/s42003-018-0256-7

    • NAID

      120006547397

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The impact of prolonged drought on phloem anatomy and phloem transport in young beech trees2018

    • Author(s)
      Dannoura Masako、Epron Daniel、Desalme Dorine、Massonnet Catherine、Tsuji Shoko、Plain Caroline、Priault Pierrick、G?rant Dominique
    • Journal Title

      Tree Physiology

      Volume: 39 Issue: 2 Pages: 201-210

    • DOI

      10.1093/treephys/tpy070

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Estimation of phloem carbon translocation belowground at stand level in a hinoki cypress stand2018

    • Author(s)
      Epron D., Dannoura M., Ishida A., Kosugi Y.
    • Journal Title

      Tree Physiology

      Volume: 00 Issue: 2 Pages: 00-00

    • DOI

      10.1093/treephys/tpy016

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The role of potassium on maize leaf carbon exportation under drought condition2017

    • Author(s)
      Martineau E., Domec JC., Bosc A., Dannoura M., Gibon Y., Bernard C., Jordan-Meille L.
    • Journal Title

      Acta Physiol Plant

      Volume: 39 Issue: 10 Pages: 219-219

    • DOI

      10.1007/s11738-017-2515-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Seasonal variations drive short-term dynamics and partitioning of recently assimilated carbon in the foliage of adult beech and pine2017

    • Author(s)
      Desalme, D., Priault, P., Gerant, D., Dannoura, M., Maillard, P., Plain, C., Epron, D.
    • Journal Title

      New Phytologyst

      Volume: 213(1) Issue: 1 Pages: 140-153

    • DOI

      10.1111/nph.14124

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 安定同位体を用いた森林における炭素循環研究-木の中のみえない炭素の流れを探る2017

    • Author(s)
      高梨聡
    • Journal Title

      生物の科学 遺伝

      Volume: 71(1) Pages: 55-62

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurements of phloem flows of forest trees using the stable carbon isotope pulse labelling techniques2016

    • Author(s)
      檀浦正子,種子田春彦,小林剛
    • Journal Title

      JAPANESE JOURNAL OF ECOLOGY

      Volume: 66 Issue: 2 Pages: 501-513

    • DOI

      10.18960/seitai.66.2_501

    • NAID

      130005406219

    • ISSN
      0021-5007, 2424-127X
    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] マツとブナの葉における非構造性炭水化物動態の日周期の違い2020

    • Author(s)
      檀浦正子、Alexandre BOSC, Dainel EPRON
    • Organizer
      第131回日本森林学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 13Cパルスラベリング手法を用いたモウソウチクの炭素輸送過程の追跡2020

    • Author(s)
      小林慧人、Shitephen Wang、檀浦正子、高梨聡、小南裕志、北山兼弘、小野田雄介
    • Organizer
      第131回日本森林学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] コナラの萌芽更新時における炭素動態2020

    • Author(s)
      高梨聡、安宅未央子、檀浦正子
    • Organizer
      第131回日本森林学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] マツの根の解剖学的構造と呼吸量2019

    • Author(s)
      檀浦正子、辻祥子
    • Organizer
      第50回根研究学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 長光路レーザー吸収分光による大気微量成分の超高感度検出とフラッ クス測定への応用2019

    • Author(s)
      高橋 けんし, 坂部綾香, 伊藤雅之, 岩田拓記, 安宅未央子, 小杉緑子
    • Organizer
      2019年度 日本分光学会年次講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] アカマツの冬季吸収炭素の放出特性2019

    • Author(s)
      高梨聡、檀浦正子、中野隆志、小南裕志、深山貴文
    • Organizer
      第66回日本生態学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 冷温帯アカマツの光合成・呼吸と生長フェノロジー2019

    • Author(s)
      高梨聡、檀浦正子、中野隆志、小南裕志、深山貴文
    • Organizer
      第130回日本森林学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 13Cパルスラベリング法を用いた、ヒノキ幼木の乾燥条件下での炭素利用2019

    • Author(s)
      辻千晃、檀浦正子、Daniel Epron、高梨聡、小南裕志、吉川彬
    • Organizer
      第130回日本森林学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 13CO2ラベリングを用いたポプラ木部における光合成産物の分布解析2019

    • Author(s)
      竹内美由紀,則定真利子,磯貝明
    • Organizer
      第69回木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Incorporation of tracer 13C to developing xylem of poplar2019

    • Author(s)
      Miyuki Takeuchi, Mariko Norisada and Akira Isogai
    • Organizer
      Cell wall meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ポプラ木部細胞壁形成における光合成産物の利用2019

    • Author(s)
      竹内美由紀,則定真利子,磯貝明
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Tracing photosynthetic carbon distribution during xylem formation2019

    • Author(s)
      Miyuki Takeuchi, Mariko Norisada and Akira Isogai
    • Organizer
      22th International Conference on Secondary Ion Mass Spectrometry (SIMS22)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 13Cパルスラベリング法を用いたヒノキ幼木の乾燥条件下での炭素利用2018

    • Author(s)
      辻千晃、檀浦正子、Daniel Epron、高梨聡、小南裕志、吉川彬
    • Organizer
      第129回日本森林学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 冷温帯アカマツの光合成・呼吸と生長フェノロジー2018

    • Author(s)
      高梨聡、檀浦正子、中野隆志、小南裕志、深山貴文
    • Organizer
      第129回日本森林学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 炭素の固定から見た木部細胞壁形成2018

    • Author(s)
      竹内 美由紀、則定 真利子、磯貝 明
    • Organizer
      第68回木材学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 乾燥ストレス条件における ヨーロッパブナの師部形状と師液輸送 Phloem anatomy and transport of carbon under drought stress in Europe Beech2017

    • Author(s)
      檀浦正子, 辻祥子, DESALME, Dorine, PRIAULT, Pierrick, PLAIN, Caroline, EPRON, Daniel
    • Organizer
      日本森林学会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 炭素安定同位体パルスラベリングを用いた水欠乏・炭素欠乏の樹木の炭素動態の解明2017

    • Author(s)
      檀浦正子
    • Organizer
      樹木の乾燥枯死・樹病枯死メカニズムの解明と温暖化等による乾燥影響評価
    • Place of Presentation
      京都大学東京オフィス(東京都千代田区)
    • Year and Date
      2017-03-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Differences in phloem anatomy alters its function under drought stress2017

    • Author(s)
      Dannoura M., Tsuji S., Desalme D., Priault P., Plain C., Epron D.
    • Organizer
      IUFRO 125th Anniversary Congress 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 13 CO2 pulse labelling experiment reveals phloem function impairment in young beech trees subjected to severe drought2017

    • Author(s)
      Desalme D., Priault P., Dannoura M., Plain C., Angeli N., MASSONNET C., Epron D.
    • Organizer
      IUFRO 125th Anniversary Congress 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Potassium nutrition and water availability affect phloem transport of photosynthetic carbon in eucalypt trees2017

    • Author(s)
      Epron D., Cabral O., Laclau J., Dannoura M., Parker Ana P., Plain C., Moreira M., Nouvellon Y.
    • Organizer
      IUFRO 125th Anniversary Congress 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Phloem conductance (and carbone translocation) at stand level: scaling anatomical data2017

    • Author(s)
      Daniel Epron, Masako Dannoura, Atsushi Ishida, Yoshiko Kosugi
    • Organizer
      iPhloem
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] How does phloem carbon translocation capacity scale with canopy photosynthesis in a hinoki cypress plantation?2017

    • Author(s)
      Dannoura M., and Epron D.
    • Organizer
      「光合成機能の環境応答とモデリング」ワークショップ
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 13Cラベリングによる光合成生産物の樹幹内転流の把握2017

    • Author(s)
      檀浦正子
    • Organizer
      「樹木年輪」研究会・組織と材質研究会合同シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 同位体イメージングによる木部細胞壁形成過程の追跡2017

    • Author(s)
      竹内 美由紀、則定 真利子、磯貝 明
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Imaging mass spectrometry analysis of woody cell wall using 13CO2 pulse labeling2017

    • Author(s)
      Miyuki Takeuchi, Mariko Norisada and Akira Isogai
    • Organizer
      253rd American Chemical Society National meeting & exposition
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Seasonality of carbon flow in a pine tree estimated using in situ 13C pulse-labeling method2016

    • Author(s)
      Takanashi, S.、Dannoura, M.、Nakano, T.、Kominami, Y.、Miyama, T.
    • Organizer
      IUFRO Regional Congress for Asia and Oceania 2016
    • Place of Presentation
      Beijin(China)
    • Year and Date
      2016-10-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] コナラにおける葉内炭素動態-13CパルスラベリングとNanoSIMSを用いてー2016

    • Author(s)
      檀浦正子・田中千晴・安宅未央子・高梨聡・竹内美由紀・吉村謙一・小南裕志・Priault Pierrick
    • Organizer
      第127回 日本森林学会
    • Place of Presentation
      神奈川県藤沢市、日本大学生物資源科学部
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 二酸化炭素安定同位体を用いた樹体内炭素動態の解明2016

    • Author(s)
      高梨聡・檀浦正子・中野隆志・小南裕志・深山貴文
    • Organizer
      第127回 日本森林学会
    • Place of Presentation
      神奈川県藤沢市、日本大学生物資源科学部
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Tracing photosynthetic carbon in leaves with nanoSIMS after 13CO2 labelling2015

    • Author(s)
      Dannoura M.,Takeuchi M., Kominami Y., Takanashi S., Yoshimura K., Ataka M.
    • Organizer
      European Geosciences Union General Assembly 2015
    • Place of Presentation
      ウィーン、オーストリア
    • Year and Date
      2015-04-12
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Book] Toward Future Earth (分担執筆)2016

    • Author(s)
      Dannnoura M., Bosc A., Epron D(chapter2) (eds.) Katsumi Takeshi and Shizuka Hashimoto
    • Total Pages
      214
    • Publisher
      Kaisei Publishing Co.,Ltd.
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2022-11-04  

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