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Genetic and chemical approach to the stage dimorphism of the pine wood nematode: the primary factor of expanding damage of pine wilt disease

Research Project

Project/Area Number 15H04514
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Forest science
Research InstitutionKyoto University

Principal Investigator

Takeuchi-Kaneko Yuko  京都大学, 農学研究科, 助教 (80452283)

Co-Investigator(Kenkyū-buntansha) 菊地 泰生  宮崎大学, 医学部, 准教授 (20353659)
神崎 菜摘  国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (70435585)
福田 健二  東京大学, 大学院農学生命科学研究科(農学部), 教授 (30208954)
土原 和子  東北学院大学, 教養学部, 准教授 (10300823)
Research Collaborator TANAKA Suguru  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywordsマツノザイセンチュウ / 分散型 / 増殖型 / 近交系 / トランスクリプトーム
Outline of Final Research Achievements

The present study aimed to clarify the switching mechanism from the propagative to the dispersal stages in the pine wood nematode, Bursaphelenchus xylophilus, the pathogen of pine wilt disease. We artificially induced the third-stage dispersal juveniles by adding crude nematode extracts and then analyzed the stage-specific transcriptome by RNA-seq technique to describe the gene expression dynamics during the stage transition. The results suggested that B. xylophilus has a chemical-signal mediated mechanism of induction of the dispersal stages, which is comparable with the dauer induction in the model organism Caenorhabditis elegans.

Academic Significance and Societal Importance of the Research Achievements

本研究の結果、マツノザイセンチュウの増殖型から分散型への人為的誘導に初めて成功し、この移行を司る因子が線虫由来の化学シグナルであることを突き止めた。これはすなわち、単木での発病から森林蔓延型の流行病化へとつながるマツノザイセンチュウの移動分散過程の鍵を握るのが特定の化学物質であることを示唆している。新規に開発した純度の高い線虫RNAの抽出法や精密な実験に適した線虫近交系の作出と併せて、今後のマツ枯れ研究に大きく寄与するものと考える。

Report

(4 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (47 results)

All 2019 2018 2017 2016 2015

All Journal Article (15 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 15 results,  Open Access: 6 results,  Acknowledgement Compliant: 5 results) Presentation (30 results) (of which Int'l Joint Research: 8 results) Book (2 results)

  • [Journal Article] Transition of Diagnostic Methods of Pine Wilt Disease2019

    • Author(s)
      竹内祐子
    • Journal Title

      Journal of the Japanese Forest Society

      Volume: 101 Issue: 1 Pages: 17-25

    • DOI

      10.4005/jjfs.101.17

    • NAID

      130007622237

    • ISSN
      1349-8509, 1882-398X
    • Year and Date
      2019-02-01
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Stage-specific transcriptome of Bursaphelenchus xylophilus reveals temporal regulation of effector genes and roles of the dauer-like stages in the lifecycle2019

    • Author(s)
      Suguru E. Tanaka, Mehmet Dayi, Yasunobu Maeda, Isheng J. Tsai, Ryusei Tanaka, Mark Bligh, Yuko Takeuchi-Kaneko, Kenji Fukuda, Natsumi Kanzaki, Taisei Kikuchi
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1

    • DOI

      10.1038/s41598-019-42570-7

    • NAID

      120006818695

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Some additional bionomic characters of Deladenus nitobei2018

    • Author(s)
      Natsumi Kanzaki, Suguru E. Tanaka, Masaaki Ito, Koji Tanaka, Bernard Slippers, and Masanobu Tabata
    • Journal Title

      Nematology

      Volume: In press Issue: 6 Pages: 597-599

    • DOI

      10.1163/15685411-00003168

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Tolerance to oxidative stress of inbred strains of the pine wood nematode, Bursaphelenchus xylophilus, differing in terms of virulence2018

    • Author(s)
      Taisuke Ekino, Suguru E. Tanaka, Natsumi Kanzaki, and Yuko Takeuchi-Kaneko
    • Journal Title

      Nematology

      Volume: In press Issue: 6 Pages: 539-546

    • DOI

      10.1163/15685411-00003158

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Strongest grip on the rod: tarsal morphology and attachment of Japanese pine sawyer beetles2017

    • Author(s)
      Voigt Dagmar、Takanashi Takuma、Tsuchihara Kazuko、Yazaki Kenichi、Kuroda Katsushi、Tsubaki Remi、Hosoda Naoe
    • Journal Title

      Zoological Letters

      Volume: 3 Issue: 1 Pages: 1-1

    • DOI

      10.1186/s40851-017-0076-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transmission electron microscopic observation of body cuticle structures of phoretic and parasitic stages of Parasitaphelenchinae nematode2017

    • Author(s)
      Taisuke Ekino, Toyoshi Yoshiga, Yuko Takeuchi-Kaneko, Natsumi Kanzaki
    • Journal Title

      PLoS ONE

      Volume: 12 Issue: 6 Pages: e0179465-e0179465

    • DOI

      10.1371/journal.pone.0179465

    • NAID

      120006822484

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In situ observation of pinewood nematode in wood2017

    • Author(s)
      Umebayashi, T., Yamada, T., Fukuhara, K., Endo, R., Kusumoto, D., Fukuda, K.
    • Journal Title

      European Journal of Plant Pathology

      Volume: 147 Issue: 2 Pages: 463-467

    • DOI

      10.1007/s10658-016-1013-8

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Artificial induction of third-stage dispersal juveniles of Bursaphelenchus xylophilus, using newly established inbred lines.2017

    • Author(s)
      Suguru E. Tanaka, Takuya Aikawa, Yuko Takeuchi-Kneko, Kenji Fukuda, and Natsumi Kanzaki
    • Journal Title

      PLoS ONE

      Volume: 12 Issue: 10 Pages: e0187127-e0187127

    • DOI

      10.1371/journal.pone.0187127

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bursaphelenchus kesiyae n. sp (Nematoda: Aphelenchoididae), isolated from dead wood of Pinus kesiya Royle ex Gordon (Pinaceae) from Vietnam, with proposal of new subgroups in the B. fungivorus group2016

    • Author(s)
      Kanzaki, N., Aikawa, T., Maehara, N. & Thu, P.Q.
    • Journal Title

      Nematology

      Volume: 18 Issue: 3 Pages: 133-146

    • DOI

      10.1163/15685411-00003051

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Morphological characters of dauer juveniles of three species of Bursaphelenchus Fuchs, 19372016

    • Author(s)
      Natsumi Kanzaki, Noritoshi Maehara, Mitsuteru Akiba, Suguru E. Tanaka, and Tatsuya Ide
    • Journal Title

      Nematology

      Volume: 18 Issue: 2 Pages: 209-220

    • DOI

      10.1163/15685411-00002954

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Diplogasteroides nix n. sp. (Nematoda: Diplogastridae), a cryptic species related to D. andrassyi, isolated from Monochamus urussovii (Coleoptera: Cerambycidae) from Hokkaido, Japan, with remarks on the body surface structures2016

    • Author(s)
      Natsumi Kanzaki, Hironori Sakamoto and Noritoshi Maehara
    • Journal Title

      Nematology

      Volume: 18 Issue: 7 Pages: 753-773

    • DOI

      10.1163/15685411-00002990

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bursaphelenchus kesiyae n. sp. (Nematoda: Aphelenchoididae), isolated from dead wood of Pinus kesiya Royle ex Gordon (Pinaceae) from Vietnam, with proposal of new subgroups in the B. fungivorus group2016

    • Author(s)
      Kanzaki, N., Aikawa, T., Maehara, N. and Thu, P. Q.
    • Journal Title

      Nematology

      Volume: 18 Issue: 2 Pages: 133-146

    • DOI

      10.1163/15685411-00002949

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Transcriptional and morphological changes in the transition from mycetophagous to phytophagous phase in a plant parasitic nematode Bursaphelenchus xylophilus.2015

    • Author(s)
      Isheng J. Tsai1, Ryusei Tanaka, Natsumi Kanzaki, Mitsuteru Akiba, Toshiro Yokoi, Margarida Espada, John T. Jones and Taisei Kikuchi
    • Journal Title

      Molecular Plant Pathology

      Volume: 未定 Issue: 1 Pages: 77-83

    • DOI

      10.1111/mpp.12261

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Bursaphelenchus sakishimanus n. sp. (Tylenchomorpha: Aphelenchoididae) isolated from a stag beetle, Dorcus titanus sakishimanus Nomura (Coleoptera: Lucanidae), on Ishigaki Island, Japan2015

    • Author(s)
      Kanzaki, N., Okabe, K. and Kobori, Y.
    • Journal Title

      Nematology

      Volume: 17 Issue: 5 Pages: 531-542

    • DOI

      10.1163/15685411-00002887

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Genome-wide variation in the pinewood nematode Bursaphelenchus xylophilus and its relationship with pathogenic traits.2015

    • Author(s)
      Palomares-Rius JE, Tsai IJ, Karim N, Akiba M, Kato T, Maruyama H, Takeuchi Y, Kikuchi T.
    • Journal Title

      BMC Genomics

      Volume: 16 Issue: 1 Pages: 1845-1845

    • DOI

      10.1186/s12864-015-2085-0

    • NAID

      120005758216

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] MRI を用いたエンボリズムの発生・回復過程における水分通導の可視化2019

    • Author(s)
      平川雅文・松下範久・福田健二・寺田康彦
    • Organizer
      第130 回日本森林学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コンパクトMRI を用いた樹幹内水分布の非破壊的観察手法の多樹種への適用2019

    • Author(s)
      小笠真由美・平川雅文・市原優・矢崎健一・飛田博順・福田健二
    • Organizer
      第130 回日本森林学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 線形動物門における運動関連器官の性的二型2019

    • Author(s)
      浴野泰甫・吉賀豊司・竹内祐子・市原 優・神崎菜摘
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 線虫はどのようにして共食いを回避しているか?2018

    • Author(s)
      浴野泰甫・吉賀豊司・竹内祐子・神崎菜摘
    • Organizer
      第65回日本生態学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 線虫のクチクラ構造と生活史特性の関係2018

    • Author(s)
      浴野泰甫・吉賀豊司・竹内祐子・市原優・神崎菜摘
    • Organizer
      第129 回日本森林学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マツノザイセンチュウ分散型III期幼虫の人為的誘導とその遺伝子発現解析2018

    • Author(s)
      田中克・神崎菜摘・菊地泰生・相川拓也・竹内祐子・福田健二
    • Organizer
      第129 回日本森林学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 針葉樹2種の苗木において根切りと水ストレスが通水に及ぼす影響2018

    • Author(s)
      梅林利弘・福田健二・内海泰弘・楠本 大・佐野雄三
    • Organizer
      第129 回日本森林学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コンパクトMRI を用いた苗木樹幹の木部樹液流速分布の日変化測定2018

    • Author(s)
      平川雅文・市橋隆自・福田健二・寺田康彦
    • Organizer
      第129 回日本森林学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マツノザイセンチュウからの簡便かつ効率的なRNA抽出手法2018

    • Author(s)
      田中克・鹿島誠・横井寿郎・神崎菜摘・永野惇
    • Organizer
      日本線虫学会第26回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 捕食性の進化に伴うクチクラ微細構造の変化2018

    • Author(s)
      浴野泰甫・吉賀豊司・竹内祐子・市原 優・神崎菜摘
    • Organizer
      日本線虫学会第26回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The cuticle ultrastructure of aphelenchoidid predators2018

    • Author(s)
      Taisuke Ekino, Yoshiga Toyoshi, Yuko Takeuchi-Kaneko, Yu Ichihara, Natsumi Kanzaki
    • Organizer
      33th Symposium of the European Society of Nematologists Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Caterpillar mechanoreceptors: morphology, distribution and mechanical response of sensilla in various lepidopetran larvae2018

    • Author(s)
      Takuma Takanashi, Kazuo Yamazaki, Shinji Sugiura, Takenari Inoue, Kazuko Tsuchihara
    • Organizer
      The 5th Annual Meeting of the Society for Bioacoustics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マツノザイセンチュウからの簡易RNA抽出法2017

    • Author(s)
      田中 克・横井寿郎・神崎菜摘・福田健二
    • Organizer
      第128回日本森林学会大会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] マツノザイセンチュウの増殖力因子の解析2017

    • Author(s)
      保谷剛志・田中 克・浴野泰甫・中村慎崇・竹内祐子
    • Organizer
      第128回日本森林学会大会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] Transmission Electron Microscopic observation of body cuticle structures of phoretic and parasitic stages of Parasitaphelenchinae nematodes2017

    • Author(s)
      Taisuke Ekino, Toyoshi Yoshiga, Yuko Takeuchi-Kaneko, Natsumi Kanzaki
    • Organizer
      Twelfth International Symposium of the Russian Society of Nematologists “Nematodes and other Ecdysozoa under the growing ecological footprint on ecosystems"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マツノザイセンチュウ分泌物の分散型III期誘導活性2017

    • Author(s)
      田中克・相川拓也・竹内祐子・福田健二・神崎菜摘
    • Organizer
      日本線虫学会第25回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Deladenus nitobei及びParasitaphelenchus sp.の角皮微細構造の性的二型と行動様式との関係2017

    • Author(s)
      浴野泰甫・吉賀豊司・竹内祐子・神崎菜摘
    • Organizer
      日本線虫学会第25回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マツ枯れの発病から蔓延への移行時の病原体線虫の戦略転換2017

    • Author(s)
      田中克・相川拓也・竹内祐子・福田健二・神崎菜摘
    • Organizer
      第22回樹木医学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] コンパクトMRIを用いた樹幹の樹液流速分布の日変化測定2017

    • Author(s)
      平川雅文・市橋隆自・福田健二・寺田康彦
    • Organizer
      第22回樹木医学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マツノザイセンチュウ全ステージの網羅的遺伝子発現解析2016

    • Author(s)
      田中 克・田中龍聖・竹内祐子・神崎菜摘・菊地泰生・福田健二
    • Organizer
      第24回日本線虫学会
    • Place of Presentation
      東京農工大学 (東京都小金井市)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] マツノザイセンチュウの増殖力因子の解析2016

    • Author(s)
      保谷剛志・田中 克・浴野泰甫・竹内祐子
    • Organizer
      第24回日本線虫学会
    • Place of Presentation
      東京農工大学 (東京都小金井市)
    • Year and Date
      2016-09-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] Current situation of pine wilt disease in southeast Louisiana, U.S.A.2016

    • Author(s)
      Yuko Takeuchi-Kaneko, Edward C. McGawley, Charles Overstreet
    • Organizer
      2016 IUFRO Working Party 7.02.10 Pine Wilt Disease International Symposium
    • Place of Presentation
      Seoul (South Korea)
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of propagation factors of the pine wood nematode, Bursaphelenchus xylophilus2016

    • Author(s)
      Tsuyoshi Hoya, Suguru E. Tanaka, Taisuke Ekino, Yuko Takeuchi-Kaneko
    • Organizer
      2016 IUFRO Working Party 7.02.10 Pine Wilt Disease International Symposium
    • Place of Presentation
      Seoul (South Korea)
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Stage-specific transcriptomic analysis of the pine wood nematode, Bursaphelenchus xylophilus2016

    • Author(s)
      Suguru E. Tanaka, Ryusei Tanaka, Yuko Takeuchi-Kaneko, Kenji Fukuda, Natsumi Kanzaki, and Taisei Kikuchi
    • Organizer
      European Society of Nematologists 32nd Symposium
    • Place of Presentation
      Braga (Portugal)
    • Year and Date
      2016-08-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] カミキリによって誘導された耐久型線虫の遺伝子発現変化2016

    • Author(s)
      田中 克・田中 龍聖・竹内 祐子・神崎 菜摘・菊地 泰生・福田 健二
    • Organizer
      第127回日本森林学会大会
    • Place of Presentation
      日本大学(神奈川県藤沢市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] マツノザイセンチュウの増殖力因子の解析2016

    • Author(s)
      保谷 剛志・田中 克・浴野 泰甫・竹内 祐子
    • Organizer
      第127回日本森林学会大会
    • Place of Presentation
      日本大学(神奈川県藤沢市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] マツノザイセンチュウT4系統及びその純系株における分散型III期関連形質の調査2015

    • Author(s)
      田中 克・神崎 菜摘・相川 拓也・田中 龍聖・竹内 祐子・福田 健二
    • Organizer
      日本線虫学会第23回大会
    • Place of Presentation
      中部大学三浦記念会館 (愛知県名古屋市)
    • Year and Date
      2015-09-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] マツノザイセンチュウ組換え近交系の孵化率の病原力への寄与2015

    • Author(s)
      保谷 剛志・田中 克・浴野 泰甫・竹内 祐子
    • Organizer
      日本線虫学会第23回大会
    • Place of Presentation
      中部大学三浦記念会館 (愛知県名古屋市)
    • Year and Date
      2015-09-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] Increase in the incidence of symptoms of pine wilt disease in southeast Louisiana2015

    • Author(s)
      Edward C. McGawley, Yuko Takeuchi, Charles C. Overstreet
    • Organizer
      Society of Nematologists Fifty-fourth Annual Meeting
    • Place of Presentation
      East Lansing, Michigan (USA)
    • Year and Date
      2015-07-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An ‘omics’ based approach to the pathogenic mechanism of pine wilt disease2015

    • Author(s)
      Yuko Takeuchi, Akira Kaneko, Tetsuro Kato, Mie N. Honjo, Atsushi J. Nagano, Hiroshi Kudoh, Kazuki Mori, Taisei Kikuchi, Satoru Kuhara, Kazuyoshi Futai
    • Organizer
      Society of Nematologists Fifty-fourth Annual Meeting
    • Place of Presentation
      East Lansing, Michigan (USA)
    • Year and Date
      2015-07-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Book] マツの絵本2016

    • Author(s)
      福田健二編
    • Total Pages
      40
    • Publisher
      農山漁村文化協会
    • Related Report
      2015 Annual Research Report
  • [Book] CHAPTER 7: Pine Wilt and Red Ring, Lethal Plant Diseases Caused by Insect-Mediated Bursaphelenchus Nematodes. In: Vector-Mediated Transmission of Plant Pathogens2016

    • Author(s)
      Natsumi Kanzaki and Robin M. Giblin-Davis (edited by Judith K. Brown)
    • Total Pages
      510
    • Publisher
      APS Books
    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2020-03-30  

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