Real-time imaging analysis of highly ordered structure of cell wall in trees

• Project/Area Number: 15H04527
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Wood science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Funada Ryo 東京農工大学, (連合)農学研究科(研究院), 教授 (20192734)
• Co-Investigator(Kenkyū-buntansha): 梶田 真也 東京農工大学, (連合)農学研究科(研究院), 教授 (40323753) ; 半 智史 東京農工大学, (連合)農学研究科(研究院), 准教授 (40627709) ; 渡邊 宇外 千葉工業大学, 先進工学部, 准教授 (70337707)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 木質バイオマス / 細胞壁 / 管状要素 / 微小管 / 培養細胞 / リグニン / 植物ホルモン / 生体イメージング / 植物細胞壁 / 細胞骨格 / セルロースミクロフィブリル / リグニン沈着 / セルロース / 修飾構造 / 二次木部 / 生体情報イメージング / 有縁壁孔

Outline of Final Research Achievements

For the highly utilization of wood biomass, we need to understand the mechanism of differentiation of secondary xylem in trees such as the process of formation of highly ordered structure of cell wall. We developed new in vitro model system that induced directly secondary xylem-like tracheary elements from cultured cells of trees such as hybrid poplar and some conifers. Tracheary elements formed highly developed structure of secondary walls, such as broad regions of thick cell walls, bordered pits and helical thicknenings. Using this model system, we analyzed the pattern of lignification in cell walls and cell corners by histochemical techniques. In addition, we analyzed the localization of microtubules using stable expression of genes for microtubule-associated proteins with green fluorescent protein. The microtubules play an important role in the determination of localization of secondary wall.

Research Products

• [Int'l Joint Research] Universitas Gadjah Mada(インドネシア)
• [Int'l Joint Research] Bangladesh Agricultural University(バングラデシュ)
• [Int'l Joint Research] Nanyang Technological University(シンガポール)
• [Journal Article] Climate change and the regulation of wood formation in trees by temperature (2018)
  • Author(s): Begum, S., Kudo, K., Rahman, Md H., Nakaba, S., Yamagishi, Y., Nabeshima, E., Nugroho, D.W., Oribe, Y., Kitin, P., Jin H.O., Funada, R.
  • Journal Title: Trees-Structure and Function Volume: 32 Issue: 1 Pages: 3-15
  • DOI: 10.1007/s00468-017-1587-6
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Formation of new networks of earlywood vessels in seedlings of the deciduous ring-porous hardwood Quercus serrata in springtime (2018)
  • Author(s): Kudo Kayo、Utsumi Yasuhiro、Kuroda Katsushi、Yamagishi Yusuke、Nabeshima Eri、Nakaba Satoshi、Yasue Koh、Takata Katsuhiko、Funada Ryo
  • Journal Title: Trees Volume: 印刷中 Issue: 3 Pages: 725-734
  • DOI: 10.1007/s00468-018-1667-2
  • Peer Reviewed
• [Journal Article] Stem gravitropism and tension wood formation in Acacia mangium seedlings inclined at various angles (2018)
  • Author(s): Nugroho, W.D., Nakaba, S., Yamagishi, Y., Begum, S., Rahman, M.H., Kudo, K., Marsoem, S.N., Funada, R.
  • Journal Title: Annals of Botany Volume: 印刷中 Issue: 1 Pages: 87-94
  • DOI: 10.1093/aob/mcy056
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Changes in the morphology and functions of vacuoles during the death of ray parenchyma cells in Cryptomeria japonica (2018)
  • Author(s): Arakawa, I., Funada, R., Nakaba, S.
  • Journal Title: Journal of Wood Science Volume: 印刷中 Issue: 3 Pages: 177-185
  • DOI: 10.1007/s10086-017-1692-6
  • NAID: 40021568605
  • Peer Reviewed
• [Journal Article] Locally heated dormant cambium can re-initiate cell production independently of new shoot growth in a deciduous conifer, Larix kaempferi (2017)
  • Author(s): Oribe, Y., Funada, R
  • Journal Title: Dendrochronologia Volume: 46 Pages: 14-23
  • DOI: 10.1016/j.dendro.2017.09.001
  • Peer Reviewed
• [Journal Article] Responses of ray parenchyma cells to wounding differ between earlywood and latewood in the sapwood of Cryptomeria japonica (2017)
  • Author(s): Nakaba, S., Morimoto, H., Arakawa, I., Yamagishi, Y., Nakada, R., Funada, R.
  • Journal Title: Trees Volume: 31 Issue: 1 Pages: 31-39
  • DOI: 10.1007/s00468-016-1452-z
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Partial desiccation enhances induction of secondary xylem-like tracheary elements from calli of hybrid poplar (Populus sieboldii x P. grandidentata). (2016)
  • Author(s): Yusuke Yamagishi, Joto Yoshimoto, Suzuka Ide, Satoshi Nakaba, Eri Nabeshima, Ugai Watanabe, Ryo Funada
  • Journal Title: Trees-structure and function Volume: - Issue: 3 Pages: 1083-1089
  • DOI: 10.1007/s00468-016-1411-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Agatharesinol biosynthesis-related changes of ray parenchyma in sapwood sticks of Cryptomeria japonica during cell death (2016)
  • Author(s): Satoshi Nakaba, Izumi Arakawa, Hikaru Morimoto, Nobumasa Bito, Takanori Imai, Ryogo Nakada, Ryo Funada
  • Journal Title: Planta Volume: 243 Issue: 5 Pages: 1225-1236
  • DOI: 10.1007/s00425-016-2473-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Relationship between the earlywood-to-latewood transition and changes in levels of stored starch around the cambium in locally heated stems of the evergreen conifer Chamaecyparis pisifera (2016)
  • Author(s): Md Hasnat Rahman, Shahanara Begum, Satoshi Nakaba, Yusuke Yamagishi, Kayo Kudo, Eri Nabeshima, Widyanto Dwi Nugroho, Yuichiro Oribe, Ryo Funada
  • Journal Title: Trees－Structure and Function Volume: - Issue: 5 Pages: 1619-1630
  • DOI: 10.1007/s00468-016-1395-4
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Earlywood vessels in ring-porous deciduous hardwoods become functional for water transport after bud burst and before the maturation of the current-year leaves (2016)
  • Author(s): Kitin, P., Funada, R.
  • Journal Title: IAWA Journal Volume: 37 Issue: 2 Pages: 315-331
  • DOI: 10.1163/22941932-20160136
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Localized cooling of stems induces latewood formation and cambial dormancy during seasons of active cambium in conifers (2016)
  • Author(s): Shahanara Begum, Kayo Kudo, Yugo Matsuoka, Satoshi Nakaba, Yusuke Yamagishi, Eri Nabeshima, Md. Hasnat Rahman, Widyanto Dwi Nugroho, Yuichiro Oribe, Hyun-O Jin, Ryo Funada
  • Journal Title: Annals of Botany Volume: 117 Issue: 3 Pages: 465-477
  • DOI: 10.1093/aob/mcv181
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cavitation of intercellular spaces is critical to establishment of hydraulic properties of compression wood of Chamaecyparis obtusa seedlings (2016)
  • Author(s): Satoshi Nakaba, Asami Hirai, Kayo Kudo, Yusuke Yamagishi, Kenichi Yamane, Katsushi Kuroda, Widyanto Dwi Nugroho, Peter Kitin, Ryo Funada
  • Journal Title: Annals of Botany Volume: 117 Issue: 3 Pages: 457-463
  • DOI: 10.1093/aob/mcv195
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Relationship between f ormation of earlywood vessels and leaf phenology in two ring-porous hardwoods, Quercus serrata and Robinia pseudoacacia, in early spring (2015)
  • Author(s): Kudo, K., Yasue, K., Hosoo, Y., Funada, R.
  • Journal Title: Journal of Wood Science Volume: 61 Issue: 5 Pages: 455-464
  • DOI: 10.1007/s10086-015-1487-6
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] トチノキ培養細胞を用いたせん孔をもつ管状要素の分化誘導 (2018)
  • Author(s): 山岸祐介，鎌田 裕，荒川圭太，佐野雄三，工藤佳世，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] 蛍光プローブによるポプラ培養細胞の管状要素誘導系におけるリグニン沈着の可視化 (2018)
  • Author(s): 吉田裕子，山岸祐介，飛松裕基，梅澤俊明，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] 心材形成過程の解析への自家蛍光を用いたスペクトルイメージングの応用 (2018)
  • Author(s): 半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] 枝打ちがヒノキの早晩材付近の仮道管形態に与える影響 (2018)
  • Author(s): 葭葉 司，平野 優，小林 元，安江 恒，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] ウルシの種子を用いた組織培養による植物体再生に関する研究 (2018)
  • Author(s): 塚田健太郎，岡田健汰，半 智史，船田 良，山岸祐介，田端雅進
  • Organizer: 第68回日本木材学会大会
• [Presentation] トドマツ不定胚形成細胞（ESM）からの不定胚形成および植物体再生に関する研究 (2018)
  • Author(s): 秋山佳貴，塚田健太郎，山岸祐介，中田了五，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] ウルシの種子からのカルス誘導と有用物質の解析 (2018)
  • Author(s): 岡田健汰，塚田健太郎，吉田裕子，Md Hasnat Rahman，田端雅進，船田 良，半 智史
  • Organizer: 第68回日本木材学会大会
• [Presentation] Wood formation in tropical trees: understanding the changes in cambial activity in relation to the rainfall pattern in hardwood trees grown in Yogyakarta, Java Island, Indonesia (2018)
  • Author(s): MD Hasnat Rahman，Widyanto Dwi Nugroho，Satoshi Nakaba，Yusuke Yamagishi，Shahanara Begum，Sri Nugroho Marsoem，Ryo Funada
  • Organizer: 第68回日本木材学会大会
• [Presentation] オーキシンの移動の制御を目的とした剥皮およびNPA塗布処理による形成層細胞の変化 (2018)
  • Author(s): 吉永恵理子，Peter Kitin，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] ゲッケイジュのカルス培養およびオイゲノールの分析に関する研究 (2018)
  • Author(s): 平木李奈，秋山佳貴，塚田健太郎，吉田裕子，Nuoendagula，梶田真也，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] チークの引張あて材における組織学的特徴の解析 (2018)
  • Author(s): 北見夏海斗，Widyanto Dwi Nugroho，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] ドリアン属のタイルセルの解剖学的特徴とその分化過程 (2018)
  • Author(s): 藤井美帆，Widyanto Dwi Nugroho，安部 久，半 智史，船田 良
  • Organizer: 第68回日本木材学会大会
• [Presentation] 樹木木部細胞壁の形成機構 (2018)
  • Author(s): 船田 良, 山岸祐介, 半 智史
  • Organizer: セルロース学会北海道・東北支部セミナー
  • Invited
• [Presentation] Effects of the application of exogenous auxin and defoliation on cambial reactivation and xylem differentiation in locally heated stems of Abies homolepis seedlings (2017)
  • Author(s): Md Hasnat Rahman, Shahanara Begum, Kayo Kudo, Takahiro Muraishi, Yusuke Yamagishi, Satoshi Nakaba, Ryo Funada
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] ドロノキ放射柔細胞の細胞死に関連するプロテアーゼの遺伝子発現解 (2017)
  • Author(s): 半 智史、高田直樹、高橋大輔、中田了五、上村松生、船田 良
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] カラマツ放射柔細胞の分化過程とデンプン貯蔵機能発現の関連性 (2017)
  • Author(s): 荒川 泉、安江 恒、船田 良、半 智史
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] 交雑ポプラ培養細胞を用いたin vitro管状要素誘導に合成オーキシンNAAおよび2,4-Dが及ぼす影響 (2017)
  • Author(s): 山岸祐介、鎌田 裕、工藤佳世、半 智史、船田 良、荒川圭太、佐野雄三
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] ジベレリンおよびブラシノステロイド処理によるヤチダモおよびヤマザクラの引張あて材様組織の誘導に関する研究 (2017)
  • Author(s): 村石貴彦、工藤佳世、山岸祐介、Widyanto Dwi Nugroho、船田 良、半 智史
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] サワラ樹幹に対する局所的な冷却処理やヒノキ苗木に対する水分制限処理が形成層細胞の分裂活動や木部分化過程に対して与える影響 (2017)
  • Author(s): 中村祐輔、Md Hasnat Rahman、半 智史、Shahanara Begum、船田 良
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] トドマツ成熟種子由来の不定胚形成細胞を経由した植物体再生に関する研究 (2017)
  • Author(s): 秋山佳貴、塚田健太郎、吉田裕子、山岸祐介、中田了五、半 智史、船田 良
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] ウルシとオオヤマレンゲの種子を用いた組織培養によるPEMs誘導に関する報告 (2017)
  • Author(s): 塚田健太郎、井上涼平、山岸祐介、沈 昱東、平野 優、安江 恒、田端雅進、半 智史、船田 良
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] 交雑ポプラ培養細胞の管状要素誘導系におけるリグニン沈着過程の解析 (2017)
  • Author(s): 吉田裕子、山岸祐介、半 智史、船田 良
  • Organizer: 第67回日本木材学会大会
  • Place of Presentation: 九州大学（福岡県福岡市）
  • Year and Date: 2017-03-17
• [Presentation] Regulation of Cambial Activity in Trees: the Role of Temperature and Precipitation (2017)
  • Author(s): Ryo Funada, Md Hasnat Rahman, Shahanara Begum, Kayo Kudo, Yusuke Yamagishi, Eri Nabeshima, Widyanto Dwi Nugroho, Sri Nugroho Marsoem, Yuichiro Oribe, Satoshi Nakaba
  • Organizer: The 9th Pacific Regional Wood Anatomy Conference
  • Int'l Joint Research
• [Presentation] Tracheary Elements in Calli of Japanese Horse Chestnut (Aesculus turbinata) Form Perforation Plates Like Structures (2017)
  • Author(s): Yusuke Yamagishi, Yutaka Kamada, Kayo Kudo, Satoshi Nakaba, Yuzou Sano, Ryo Funada
  • Organizer: The 9th Pacific Regional Wood Anatomy Conference
  • Int'l Joint Research
• [Presentation] The Pattern of Location of the First Earlywood Vessels in the Current year’s xylem in a Ring-porous Hardwood, Quercus serrata (2017)
  • Author(s): Kayo Kudo, Yuichiro Oribe, Shahanara Begum, Yusuke Yamagishi, Eri Nabeshima, Md Hasnat Rahman, Satoshi Nakaba, Katsuhiko Takata, Ryo Funada
  • Organizer: The 9th Pacific Regional Wood Anatomy Conference
  • Int'l Joint Research
• [Presentation] Initiation of Starch Accumulation in Ray Parenchyma Cells in Larix kaempferii (2017)
  • Author(s): Izumi Arakawa, Koh Yasue, Ryo Funada, Satoshi Nakaba
  • Organizer: The 9th Pacific Regional Wood Anatomy Conference
  • Int'l Joint Research
• [Presentation] Sequential analysis of rhytidome formation in Cryptomeria japonica, Quercus serrata and Cercidiphyllum japonicum (2017)
  • Author(s): Megumi Ohse, Ryo Funada, Satoshi Nakaba
  • Organizer: The 9th Pacific Regional Wood Anatomy Conference
  • Int'l Joint Research
• [Presentation] 交雑ポプラカルスにおける二次木部様管状要素の分化 (2016)
  • Author(s): 山岸祐介、吉田裕子、渡辺宇外、半 智史、船田 良、荒川圭太、佐野雄三
  • Organizer: 第34回日本植物細胞分子生物学会大会
  • Place of Presentation: 信州大学（長野県上田市）
  • Year and Date: 2016-09-01
• [Presentation] Regulation of wood formation in trees: the role of temperature in cambial activity (2016)
  • Author(s): Ryo Funada, Shahanara Begum, Kayo Kudo, Md Hasnat Rahman, Yusuke Yamagishi, Eri Nabeshima, Widyanto Dwi Nugroho, Yuichiro Oribe, Satoshi Nakaba
  • Organizer: International Academy of Wood Science Meeting
  • Place of Presentation: Academie d’Agriculture de France (Paris, France)
  • Year and Date: 2016-06-01
  • Int'l Joint Research
• [Presentation] The effects of defoliation and auxin transport inhibitor, N-(1-Naphthyl)phthalamic acid (NPA) on cambial reactivation and formation of earlywood tracheids in locally heated stems of Abies homolepis seedlings (2016)
  • Author(s): Md Hasnat Rahman, Kayo Kudo, Yusuke Yamagishi, Shahanara Begum, Michito Hosaka, Satoshi Nakaba, Ryo Funada
  • Organizer: The Korean Society of Wood Science & Technology
  • Place of Presentation: Kookmin University (Seoul, Korea)
  • Year and Date: 2016-04-15
  • Int'l Joint Research
• [Presentation] 交雑ポプラ培養細胞を用いたin vitoro管状要素誘導へのオーキシン輸送阻害剤処理の影響 (2016)
  • Author(s): 山岸祐介、吉田裕子、工藤佳世、半 智史、船田 良、荒川圭太、佐野雄三
  • Organizer: 第66回日本木材学会大会
  • Place of Presentation: 名古屋大学〔愛知県名古屋市）
  • Year and Date: 2016-03-27
• [Presentation] ポプラ培養細胞の管状要素誘導系を用いたリグニン沈着過程に関する研究 (2016)
  • Author(s): 吉田裕子、井出寿々加、吉本靖東、山岸祐介、渡辺陽子、佐野雄三、梶田真也、半 智史、船田 良
  • Organizer: 第66回日本木材学会大会
  • Place of Presentation: 名古屋大学〔愛知県名古屋市）
  • Year and Date: 2016-03-27
• [Presentation] コブシおよびシデコブシの種子由来の不定胚形成細胞を経由した植物体再生における受粉およびオーキシン濃度の影響 (2016)
  • Author(s): 栢野旭代、山岸祐介、小口あずさ、岡 加奈子、朴 仁善、戸丸信弘、半 智史、船田 良
  • Organizer: 第66回日本木材学会大会
  • Place of Presentation: 名古屋大学〔愛知県名古屋市）
  • Year and Date: 2016-03-27
• [Presentation] ウルシとオオヤマレンゲの種子を用いた組織培養による植物体再生 (2016)
  • Author(s): 塚田健太郎、栢野旭代、小口あずさ、井上涼平、山岸祐介、Yudong Shen、安江 恒、田端雅進、半 智史、船田 良
  • Organizer: 第66回日本木材学会大会
  • Place of Presentation: 名古屋大学〔愛知県名古屋市）
  • Year and Date: 2016-03-27
• [Presentation] ブラシノステロイド生合成阻害剤がヤマザクラの引張あて材形成に与える影響 (2016)
  • Author(s): 村石貴彦、工藤佳世、山岸祐介、Widyanto Dwi Nugroho、船田 良、半 智史
  • Organizer: 第66回日本木材学会大会
  • Place of Presentation: 名古屋大学〔愛知県名古屋市）
  • Year and Date: 2016-03-27
• [Presentation] The effects of defoliation and auxin transport inhibitor, N-(1-Naphthyl)phthalamic acid (NPA) on cambial reactivation and formation of earlywood tracheids in locally heated stems of Abies homolepis seedlings (2016)
  • Author(s): Md Hasnat Rahman、Kayo Kudo、Yusuke Yamagishi、Shahanara Begum、Michito Hosaka、Satoshi Nakaba、Ryo Funada
  • Organizer: 第66回日本木材学会大会
  • Place of Presentation: 名古屋大学〔愛知県名古屋市）
  • Year and Date: 2016-03-27
• [Book] Secondary xylem biology (2016)
  • Author(s): Funada, R., Yamagishi, Y., Begum, S., Kudo, K., Nabeshima, E., Nugroho,W.D., Oribe, Y., Nakaba, S.
  • Total Pages: 397
  • Publisher: Academic Press
• [Book] あて材の科学-樹木の重力応答と生存戦略- (2016)
  • Author(s): 船田 良
  • Total Pages: 350
  • Publisher: 海青社
• [Book] Plant Microtechniques: Methods and Protocols (2015)
  • Author(s): Nakaba, S., Kitin, P., Yamagishi, Y., Begum, S., Kudo, K., Nugroho,W.D., Funada, R.
  • Total Pages: 576
  • Publisher: Springer
• [Book] 生態工学ハンドブック (2015)
  • Author(s): 船田 良
  • Total Pages: 447
  • Publisher: アドスリー（丸善）
• [Remarks] 東京農工大学植物資源形成学研究室
  • URL: http://web.tuat.ac.jp/~keisei/
• [Remarks] 東京農工大学研究者情報
  • URL: http://kenkyu-web.tuat.ac.jp/Profiles/1/0000023/profile.html