| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
|
|---|
| Outline of Final Research Achievements |
As well as a myxosporean Kudoa septempunctata infecting the olive flounder Paralichthys olivaceus, several Kudoa species showing an asymptomatic infection in marine fishes have been recently suspected as causative agents of food-poisoning in humans. In the present study, epizootiological studies on K. hexapunctata from Pacific bluefin tuna Thunnus orientalis indicated that infection with K. hexapunctata were found mostly in juvenile tuna fish less than 1-year old and influenced by nursery ground, rather than origin of seedlings (natural or artificial). Pathogenicity tests with human intestinal epithelial cells (Caco-2 cells) and diarrhea-model animals (suckling mouse) proved that pathogenicity of K. hexapunctata was 1 to 50 lower than that of K. septempunctata. Finally, the lowest limit of K. hexapunctata in tuna muscle was shown to be 10 the 7th power of spores / g-muscle.
|
