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Novel sex-determining genes in fish

Research Project

Project/Area Number 15H04542
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic bioproduction science
Research InstitutionThe University of Tokyo

Principal Investigator

Kikuchi Kiyoshi  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (20292790)

Co-Investigator(Kenkyū-buntansha) 小山 喬  東京大学, 大学院農学生命科学研究科(農学部), 特別研究員 (40749701)
Co-Investigator(Renkei-kenkyūsha) TASUMI Satoshi  東京大学, 大学院農学生命科学研究科, 特任助教 (90359646)
HOSOYA Sho  東京大学, 大学院農学生命科学研究科, 助教 (60526466)
SAKAMOTO Takashi  東京海洋大学, 海洋科学技術研究科, 准教授 (40313390)
Research Collaborator MIZUNO Naoki  東京大学, 大学院農学生命科学研究科, 技術専門職員
JYO Yuka  東京大学, 大学院農学生命科学研究科, 技術専門職員
FUJITA Masashi  東京大学, 大学院農学生命科学研究科, 技術職員
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywords性決定 / 生殖 / 遺伝 / 遺伝的多様性 / 多様性
Outline of Final Research Achievements

Sex-determining genes can be used for sex identification, potentially providing important information regarding the ecology and conservation of many wild fish species, as well as management in aquaculture. Since the sex-determining gene(s) are not identified for most fish species and not well conserved among them, it has been difficult to obtain sex-specific markers for a target species of fish. In this study, we show that a combination of linkage and linkage-disequilibrium mapping implemented with high-throughput sequencing technologies is an efficient strategy for identifying novel sex-determining genes/regions in fish.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (41 results)

All 2018 2017 2016 2015

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (33 results) (of which Int'l Joint Research: 8 results,  Invited: 6 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Identification of the sex-determining locus in grass puffer (Takifugu niphobles) provides evidence for sex-chromosome turnover in a subset of Takifugu species2018

    • Author(s)
      Risa Ieda, Sho Hosoya, Shota Tajima, Kazufumi Atsumi, Takashi Kamiya, Aoi Nozawa, Yuma Aoki, Satoshi Tasumi, Takashi Koyama, Osamu Nakamura, Yuzuru Suzuki, Kiyoshi Kikuchi
    • Journal Title

      PLOSONE

      Volume: 13 Issue: 1 Pages: e0190635-e0190635

    • DOI

      10.1371/journal.pone.0190635

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mucosal IgM Antibody with D-Mannose Affinity in Fugu Takifugu rubripes Is Utilized by a Monogenean Parasite Heterobothrium okamotoi for Host Recognition2017

    • Author(s)
      Igarashi K, Matsunaga R, Hirakawa S, Hosoya S, Suetake H, Kikuchi K, Suzuki Y, Nakamura O, Miyadai T, Tasumi S, Tsutsui S.
    • Journal Title

      Journal of Immunology

      Volume: in press Issue: 10 Pages: 4107-4114

    • DOI

      10.4049/jimmunol.1601996

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The teleost parasite Heterobothrium okamotoi utilizes a mucous antibody from the host as a target molecule for host recognition2017

    • Author(s)
      Igarashi K, Matsunaga R, Hirakawa S, Hosoya S, Suetake H, Kikuchi K, Suzuki Y, Nakamura O, Miyadai T, Tasumi S, Tsutsui S
    • Journal Title

      Journal of Immunology

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two isoforms of aquaporin 2 responsive to hypertonic stress in bottlenose dolphin.2016

    • Author(s)
      Suzuki M, Wakui H, Itou T, Segawa T, Inoshima Y, Maeda K, Kikuchi K.
    • Journal Title

      J Exp Biol.

      Volume: - Pages: 1249-1258

    • DOI

      10.1242/jeb.132811

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification and characterization of pufflectin from the grass pufferfish Takifugu niphobles and comparison of its expression with that of Takifugu rubripes2016

    • Author(s)
      Tasumi S, Yamaguchi A, Matsunaga R, Fukushi K, Suzuki Y, Nakamura O, Kikuchi K, Tsutsui S
    • Journal Title

      Developmental and Comparative Immunology

      Volume: 59 Pages: 48-56

    • DOI

      10.1016/j.dci.2016.01.007

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Accumulation of cells expressing macrophage colony-stimulating factor receptor gene in the ovary of a pregnant viviparous fish, Neoditrema ransonnetii (Perciformes, Embiotocidae)2016

    • Author(s)
      Ueda K, Saito E, Iwasaki K, Tsutsui S, Nozawa A, Kikuchi K, Nakamura O
    • Journal Title

      Fish and Shellfish Immunology

      Volume: 50 Pages: 223-230

    • DOI

      10.1016/j.fsi.2016.01.028

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] 水産科学におけるゲノム情報の活用を考える2018

    • Author(s)
      菊池潔
    • Organizer
      第34回マリントキシン研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] トラフグ属魚類種間雑種の初回成熟期に関する研究2018

    • Author(s)
      木元亮太・細谷将・佐藤茉奈・水野直樹・菊池潔
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ブリ属の性決定遺伝子はステロイド代謝酵素である2018

    • Author(s)
      小山喬・森島輝・山下量平・豊田敦・内野翼・坂本崇・鈴木萌・細谷将・菊池潔
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ブリ属性決定遺伝子の機能解析2018

    • Author(s)
      小山喬・山下雄史・佐々木皓平・宇野誠一・小山次朗・田角聡志・菊池潔
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ブリ属性決定遺伝子の発現解析2018

    • Author(s)
      中本正俊・小山喬・水野直樹・菊池潔・坂本崇
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新規トラフグゲノム参照配列の作成と近縁魚種への利用2018

    • Author(s)
      大木駿・家田梨櫻・細谷将・菊池潔
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] クサフグ性決定遺伝子の探索 - 少数個体を用いたGWASの発見的利用-2018

    • Author(s)
      家田梨櫻・小山喬・細谷将・大木駿・水野直樹・藤田真志・菊池潔
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] GRAS-Di 法で明らかとなった「ゲノム再編成による新規性決定遺伝子座の誕生」2018

    • Author(s)
      藤川大学・細谷 将・小林純也・田島祥太・家田梨櫻・佐藤茉菜・城夕香・中村修・菊池潔
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ショウサイフグ性決定遺伝子の探索 -Pool-seq による表現型原因遺伝子の特定-2018

    • Author(s)
      家田梨櫻・細谷将・小山喬・城夕香・菊池潔
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ゼブラフィッシュを用いたブリ属性決定遺伝子の機能解析2018

    • Author(s)
      車遥介・小山喬・岡田恵治・家田梨櫻・ 朝比奈潔・菊池潔
    • Organizer
      平成30年度日本水産学会春季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アンプリコシーケス法による高速ジェノタイピング技術の確立2017

    • Author(s)
      佐藤茉菜・細谷将・吉川壮太・菊池潔
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京・品川)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] ターゲットキャプチャー法によるブリW染色体塩基配列決定の試み2017

    • Author(s)
      小山喬・森島輝・鈴木穣・内野翼・坂本崇・菊池潔
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京・品川)
    • Year and Date
      2017-03-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] 性決定遺伝子をNGSで簡単に決められないだろうか?2017

    • Author(s)
      菊池潔
    • Organizer
      DMY研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The reuse of the same sex chromosome in pufferfishes.2017

    • Author(s)
      Kiyoshi Kikuchi
    • Organizer
      Asian Sex Differentiation Network (7th Gonad Biology Joint Meeting, 2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Footprints of selection around the sex determining gene in fugu2017

    • Author(s)
      Kabir Ahammad, Risa Ieda, Shotaro Hirase, Takashi Koyama, Satoshi Tasumi, Sho Hosoya, Kiyoshi Kikuchi
    • Organizer
      International symposium, Fisheries Science for Future Generations
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ターゲットエンリッチメント法と長鎖シーケンサーによる領域特異的塩基配列決定の試み2017

    • Author(s)
      小山喬・森島輝・鈴木穣・内野翼・坂本崇・菊池潔
    • Organizer
      次世代シーケンサー現場の会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 水産学会賞受賞者講演 ゲノムワイド解析によるフグ性決定遺伝子の同定2017

    • Author(s)
      菊池潔
    • Organizer
      平成29年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京・品川)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 世界の水産養殖産業大躍進とその基盤技術2017

    • Author(s)
      菊池潔
    • Organizer
      日本農学会 公開シンポジウム
    • Place of Presentation
      東京大学(東京・文京)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Incipient transition of a sex determining gene in pufferfish closely-related to fugu2016

    • Author(s)
      Ieda R, Hosoya S, Tasumi S, Kobayashi H, Suzuki S, Kikuchi K
    • Organizer
      The 22nd International congress of zoology
    • Place of Presentation
      OIST(Okinawa・Onna)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genetic basis of the parallel evolution of scale loss among closely-related species of fugu2016

    • Author(s)
      Kim D, Hosoya S, Kai W, Nozawa A, Jo Y, Suzuki Y, Tasumi S, Kikuchi K
    • Organizer
      The 7th World Fisheries Congress
    • Place of Presentation
      Korea・Busan
    • Year and Date
      2016-05-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ブリ性決定遺伝子座の高精度塩基配列決定2016

    • Author(s)
      小山喬、菊池潔、内野翼、坂本崇
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京・品川
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ショウサイフグが獲得した新しい性決定遺伝子座の解析2016

    • Author(s)
      家田梨櫻、青木勇磨、中村修、城夕香、小山喬、細谷将、田角聡志、菊池潔
    • Organizer
      平成28年度日本水産学会春季大会
    • Place of Presentation
      東京・品川
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ゲノム情報は本当に水産に役立つのか? - 14 年間の定点観測から-2016

    • Author(s)
      菊池潔
    • Organizer
      第6回太平洋中海域トラフグ研究会
    • Place of Presentation
      愛知県庁舎(愛知・名古屋)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 浜名湖を中心とした日本近海のフグにおける性決定遺伝子の進化2016

    • Author(s)
      家田梨櫻・カビールアハマド・小山喬・藤田真志・平瀬翔太朗・細谷将・田角聡志・菊池潔
    • Organizer
      浜名湖をめぐる研究者の会
    • Place of Presentation
      東京大学水産実験所(静岡・浜松)
    • Related Report
      2016 Annual Research Report
  • [Presentation] NGSを用いたフグ類の性決定遺伝子の探索2016

    • Author(s)
      家田梨櫻・細谷将・小山喬・田角聡志・鈴木重則・小林久人・菊池潔
    • Organizer
      生物資源ゲノム解析拠点シンポジウム
    • Place of Presentation
      東京農業大学(東京・世田谷)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Turnover of sex chromosomes in pufferfishes2016

    • Author(s)
      Kikuchi K
    • Organizer
      The workshop on sex chromosomes in frogs and fish
    • Place of Presentation
      Hiroshima University(Hiroshima・Hiroshima)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Incipient transition of a sex-determining gene among closely related species of fugu2015

    • Author(s)
      Risa Ieda, Sho Hosoya, Satoshi Tasumi, Shigenori Suzuki, Kiyoshi Kikuchi
    • Organizer
      43rd Scientific Symposium of the United State-Japan Natural Resource Aquaculture Panel
    • Place of Presentation
      Nagasaki・Nagasaki
    • Year and Date
      2015-11-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Whole genome re-sequencing of fugu populations2015

    • Author(s)
      Sho Hosoya. Satoshi Tasumi, Kiyoshi Kikuchi
    • Organizer
      43rd Scientific Symposium of the United State-Japan Natural Resource Aquaculture Panel
    • Place of Presentation
      Nagasaki・Nagasaki
    • Year and Date
      2015-11-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 全ゲノムリシーケンスによるトラフグ集団の解析-日本海群と太平洋群の比較-2015

    • Author(s)
      細谷将、田角聡志、菊池潔
    • Organizer
      動物遺伝育種学会第16回神戸大会
    • Place of Presentation
      兵庫・神戸
    • Year and Date
      2015-11-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] 全ゲノムリシーケンスによるトラフグの集団間比較 -日本海 vs. 太平洋-2015

    • Author(s)
      細谷将、田角聡志、菊池潔
    • Organizer
      平成27年度日本水産学会秋季大会
    • Place of Presentation
      宮城・仙台
    • Year and Date
      2015-09-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 性染色体進化初期段階の実験モデルとしてのブリ類の有用性2015

    • Author(s)
      小山喬、吉田一範、尾崎照遵、青木純哉、荒木和男、細谷将、菊池潔、坂本崇
    • Organizer
      日本進化学会第17回大会
    • Place of Presentation
      東京・文京
    • Year and Date
      2015-08-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] INCIPIENT TRANSITION OF A SEX DETERMINING GENE IN Takifugu PUFFERFISH2015

    • Author(s)
      Risa Iedaa, Sho Hosoyaa, Satoshi Tasumia, Shigenori Suzukib, Kiyoshi Kikuchi
    • Organizer
      The International Symposium on Aquaculture Genetics XII
    • Place of Presentation
      Spain, Santiago de Compostela
    • Year and Date
      2015-07-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ブリ性染色体では局所的な連鎖不平衡が観察される+魚類コア遺伝子の網羅的同定の試み2015

    • Author(s)
      小山喬、吉田一範、尾崎照遵、青木純哉、細谷将、荒木和男、菊池潔、坂本崇
    • Organizer
      NGS現場の会第四回研究会
    • Place of Presentation
      茨城・つくば
    • Year and Date
      2015-07-01
    • Related Report
      2015 Annual Research Report
  • [Book] 水産遺伝育種学(担当 第11章水産育種におけるゲノム情報の利用)2017

    • Author(s)
      菊池潔(編集 中嶋正道, 荒井克俊, 岡本信明, 谷口順彦)
    • Total Pages
      243
    • Publisher
      東北大学出版会
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 220797012018

    • Inventor(s)
      坂本崇・中本正俊・菊池潔・小山喬
    • Industrial Property Rights Holder
      坂本崇・中本正俊・菊池潔・小山喬
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-027260
    • Filing Date
      2018
    • Related Report
      2017 Annual Research Report

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Published: 2015-04-16   Modified: 2019-12-27  

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