| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Outline of Final Research Achievements |
We investigated mechanisms of resistance against herpesviral hematopoietic necrosis (HVHN) in a resistant strain of goldfish and tried to develop biomarkers utilizing in selection breeding without virus infection.
Virus susceptibility of primary cell culture derived from fin of the resistant fish is significantly lower than that of sensitive strain. In resistant fish, virus load increased for 3 days after virus infection like in the sensitive fish and subsequently it drastically decreased. MHC class I gene expression was significantly higher in the resistant fish than in the sensitive fish before and after virus infection. The resistant trait showed Mendelian dominant inheritance. Genotyping-by-sequencing (GBS) method was applied to exploration of single nucleotide polymorphisms (SNP) and the linkage analysis of the SNPs showed that two SNPs were significantly related with the resistance to HVHN. Finally, we developed a qPCR for MHC I gene expression and HRM for the SNP detection.
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