| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
The characteristic of broad host range gene vector particle (VP) that enables gene transfer between phylogenetically distant organisms was investigated. First, the conjugation of VP infected Escherichia coli HfrC transductant to F- (leu pro his arg) resulted in VP production in only about half of the transconjugants, no generation of pro transconjugants and the conjugative frequency of leu adjacent to pro was reduced by 4-orders of magnitude of the control. Recognition sequence probably locates in the vicinity of the pro-leu loci on the chromosome, since the linkage was observed other than pro.
Second, VP originated from a psychrophile whose optimum at 10℃ was infected to E. coli to generate the transductant. The generated transductants showed lower optimum and permissive temperature by 7℃ and highest biomass at 10℃ revealing the transfer of characteristics of psychrophile to the mesophile by VP. These results indicate the possible function of VP in gene-transfer in nature.
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