| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Outline of Final Research Achievements |
Pufferfish accumulate high levels of tetrodotoxin (TTX) in liver and ovary. However, the underlying toxification mechanism of pufferfish is poorly understood. In this study, we developed the new method to analyze TTX by spectroscopic SPECT imaging and investigated the genes and the proteins involved in toxification of pufferfish.
We synthesized radiolabeled TTX, based on the in silico examination of radioisotope element and its binding site of TTX. Spectroscopic SPECT imaging demonstrated that after TTX was administered into non-toxic pufferfish Takifugu rubripes, the toxin was detected in liver 60 min after administration. Candidates of the genes relating to transport TTX into liver of pufferfish were elucidated and the gene encoding OCT6 is promising. TTX-binding protein with a molecular mass of 10 k was isolated and identified as vitellogenin-1 like protein. Vitellogenin is suggested to be a carrier protein of TTX from liver to ovary in pufferfish.
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