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Activation of protoplast function by nanobubbles and development of high-speed production system for useful metabolites

Research Project

Project/Area Number 15H04569
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionUniversity of Tsukuba

Principal Investigator

AOYAGI Hideki  筑波大学, 生命環境系, 教授 (00251025)

Co-Investigator(Renkei-kenkyūsha) BAMBA Takeshi  九州大学, 生体防御医学研究所, 教授 (10432444)
NAKAO Yoichi  早稲田大学, 理工学術院, 教授 (60282696)
OU Hekisyou  流通経済大学, 経済学部, 教授 (80261775)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywordsナノバブル / エチレン / プロトプラスト / バイオリアクター / 追熟 / 植物 / 微生物 / 細胞壁 / マイクロバブル / 有用物質生産
Outline of Final Research Achievements

The nanobubble generation system was originally developed. This system can generate nanobubbles of various gases with high efficiency. The generated nanobubbles could stably exist in different solutions and media. Various investigations using yeast, plants cells and protoplasts suggested that the metabolites biosynthesized by protoplasts were released into the culture broth, with the double benefits of increasing the overall productivity and facilitating downstream processing. Various nanobubbles enhance those phenomena. Application of nanobubbles to yeast, various plants cells and protoplasts resulted in efficient production of useful metabolites. The bioreactor capable of supplying nanobubbles was also developed. The nanobubbles can be also used for ripening of fruit, inactivation of microbes, removal of chemicals on fruit surface, etc.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (18 results)

All 2018 2017 2016 2015

All Journal Article (7 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 6 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Practices of shake-flask culture and advances in monitoring CO2 and O22018

    • Author(s)
      Masato Takahashi, Hideki Aoyagi
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: in press Issue: 10 Pages: 1-11

    • DOI

      10.1007/s00253-018-8922-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Stable antibacterial silver nanoparticles produced with seed-derived callus extract of Catharanthus roseus2017

    • Author(s)
      Dandy Ahamefula Osibe, Nneka Virginia Chiejina, Kazuyoshi Ogawa, Hideki Aoyagi
    • Journal Title

      Artificial Cells, Nanomedicine, and Biotechnology

      Volume: 22 Issue: 6 Pages: 1-8

    • DOI

      10.1080/21691401.2017.1367927

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of a circulation direct sampling and monitoring system for O2 and CO2 concentrations in the gas-liquid phases of shake flask systems during microbial cell culture2017

    • Author(s)
      Masato Takahashi, Yoshisuke Sawada, Hideki Aoyagi
    • Journal Title

      AMB Express

      Volume: 7 Issue: 1 Pages: 163-163

    • DOI

      10.1186/s13568-017-0464-4

    • NAID

      120007134876

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inactivation of Escherichia coli O157:H7 by treatment with different temperatures of micro-bubbles ozone containing water2017

    • Author(s)
      Chuajedton, A., Aoyagi, H., Uthaibutra, J., Pengphol, S., Whangchai, K.
    • Journal Title

      International Food Research Journal

      Volume: 24 Pages: 1006-1010

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of ozone microbubbles and ultrasonic irradiation on pesticide detoxification in tangerine cv. Sai Nam Pung2017

    • Author(s)
      Whangchai, K., Uthaibutra, J., Nuanaon, N., Aoyagi, H.
    • Journal Title

      International Food Research Journal

      Volume: 24 Pages: 1135-1139

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Production of secretory cutinase by recombinant Saccharomyces cerevisiae protoplasts2016

    • Author(s)
      Hideki Aoyagi, Yoichi Katakura, Akio Iwasaki
    • Journal Title

      Springerplus

      Volume: 5 Issue: 1 Pages: 160-165

    • DOI

      10.1186/s40064-016-1806-4

    • NAID

      120007129594

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] CHO細胞培養におけるIgG モノクローナル抗体の酸性電荷バリアントに対する温度シフトの影響2015

    • Author(s)
      岸下昇平、青柳秀紀
    • Journal Title

      バイオインダストリー

      Volume: 32 Pages: 59-65

    • Related Report
      2015 Annual Research Report
  • [Presentation] LAL固定化ビーズを用いたエンドトキシンの高感度・迅速検出法の開発と利用2017

    • Author(s)
      猪瀬陽加、青柳秀紀
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] LAL固定化ビーズ法を用いたグラム陰性菌の培養に伴うエンドトキシンの遊離特性の定量的解析(第2報)2017

    • Author(s)
      大瀧賀也、青柳秀紀
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 振盪フラスコ培養中のフラスコ気相部が培養微生物群集に及ぼす影響の解析2017

    • Author(s)
      高橋将人、青柳秀紀
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Hydrogen phosphate ion as novel accelerator and inhibitor of Maillard reaction2017

    • Author(s)
      Ajeeta Anand, Hideki Aoyagi
    • Organizer
      化学工学会 第49回秋季大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] LAL固定化ビーズを用いたエンドトキシンの超高感度・迅速測定法の開発と利用2017

    • Author(s)
      猪瀬陽加、青柳秀紀
    • Organizer
      第5回日本生物工学会東日本支部コロキウム
    • Place of Presentation
      筑波大学東京キャンパス文京校舎・東京都・文京区
    • Related Report
      2016 Annual Research Report
  • [Presentation] LAL固定化ビーズ法を用いた種々のグラム陰性菌の培養に伴うエンドトキシンの遊離特性の定量的解析2017

    • Author(s)
      大瀧賀也、青柳秀紀
    • Organizer
      第5回日本生物工学会東日本支部コロキウム
    • Place of Presentation
      筑波大学東京キャンパス文京校舎・東京都・文京区
    • Related Report
      2016 Annual Research Report
  • [Presentation] 気相および液相のCO2とO2に着目した振盪フラスコ培養法の解析2016

    • Author(s)
      高橋将人、青柳秀紀
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場・富山県・富山市
    • Related Report
      2016 Annual Research Report
  • [Presentation] LAL固定化ビーズ法を用いたエンドトキシンの高感度・迅速検出法の開発と利用2016

    • Author(s)
      猪瀬陽加、飯島綾、青柳秀紀
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場・富山県・富山市
    • Related Report
      2016 Annual Research Report
  • [Presentation] LAL固定化ビーズ法を用いたグラム陰性菌の培養に伴うエンドトキシンの遊離特性の定量的解析2016

    • Author(s)
      大瀧賀也、飯島綾、青柳秀紀
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場・富山県・富山市
    • Related Report
      2016 Annual Research Report
  • [Presentation] LAL固定化ビーズ法を用いたEscherichia coliの培養に伴うエンドトキシンの遊離特性の定量的解析2015

    • Author(s)
      飯島綾、青柳秀紀
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      鹿児島県 鹿児島市(城山観光ホテル)
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] Preparation of the ethylene nanobubbles and it's application for the useful metabolite production2015

    • Author(s)
      Hideki Aoyagi
    • Organizer
      Biotechnology International Congress 2015 “Biotechnology for healthy society”
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2015-09-10
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2015-04-16   Modified: 2022-11-04  

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