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Control of accumulation of functional phytochemicals of leaf vegetables by UV light irradiation

Research Project

Project/Area Number 15H04571
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionChiba University

Principal Investigator

Goto Eiji  千葉大学, 大学院園芸学研究科, 教授 (00186884)

Co-Investigator(Kenkyū-buntansha) 兼子 敬子 (大橋敬子)  玉川大学, 農学部, 教授 (50332599)
彦坂 晶子  千葉大学, 大学院園芸学研究科, 准教授 (50345188)
石神 靖弘  千葉大学, 大学院園芸学研究科, 助教 (50361415)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords紫外線 / 機能性成分 / 光質 / アントシアニン / 抗酸化能 / 植物工場 / 生理活性物質 / 施設園芸・植物工場 / 人工光 / 成長
Outline of Final Research Achievements

Red leaf lettuce grown under white light was used as the plant material. Three peak wavelengths (310, 325, and 340 nm) of UV lights at 0.5 Wm-2 for 16 h were added to the white light for 3 d prior to harvest. Anthocyanin concentration and ORAC value of the leaves were measured every 8 h. mRNA gene expression of chalcone synthase (CHS) and the flavonoid 3-O-glucosyltransferase (UFGT) were also analyzed every 8 h. Anthocyanin concentration was significantly higher at 310 nm compared with 325 and 340 nm. Total ORAC values of the UV treatments were higher than in the control. CHS was highest at 310 nm, followed by 325 nm. UFGT increased with time, similarly to the anthocyanin concentration. These results indicated that UV-B light stimulated the biosynthesis of anthocyanin and other antioxidant polyphenols. From this research, we concluded that addition of UV light 1 to 3 d prior to harvest is effective for the production of functional phytochemical rich lettuce.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (4 results)

All 2016

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Effect of UV light on phytochemical accumulation and expression of anthocyanin biosynthesis genes in red leaf lettuce2016

    • Author(s)
      Eiji Goto, Knako Hayashi, Shinichi Furuyama, Sshoko Hikosaka and Yasuhiro Ishigami
    • Journal Title

      Acta Horticulturae

      Volume: 1134 Issue: 1134 Pages: 179-185

    • DOI

      10.17660/actahortic.2016.1134.24

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Effect of UV Light on Phytochemical Accumulation and Antioxidant Capacity in Red Leaf Lettuce2016

    • Author(s)
      Eiji Goto
    • Organizer
      国際会議「SSLCHINA 2016(第13回中国固体素子照明国際フォーラム」
    • Place of Presentation
      中国北京市
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effect of UV Light on Phytochemical Accumulation and Expression of Anthocyanin Biosynthesis Genes in Red Leaf Lettuce2016

    • Author(s)
      Eiji Goto, Hayashi, K., S. Furuyama, Hikosaka, S., Ishigami, Y.
    • Organizer
      8th International Symposium on Light in Horticulture(国際園芸学会)
    • Place of Presentation
      米国ミシガン大学
    • Year and Date
      2016-06-23
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of UV Light on Phytochemical Accumulation and Expression of Anthocyanin Biosynthesis Genes in Red Leaf Lettuce2016

    • Author(s)
      E. Goto, K. Hayashi, S. Furuyama, S. Hikosaka and Y. Ishigami
    • Organizer
      VIII International Symposium on Light in Horticulture
    • Place of Presentation
      米国ミシガン州立大学(米国、ミシガン州)
    • Year and Date
      2016-05-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2019-03-29  

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