Tissue specific lipid accumulation in response to amino acid signal
| Project/Area Number |
15H04583
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Hakuno Fumihiko 東京大学, 大学院農学生命科学研究科(農学部), 助教 (30282700)
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| Co-Investigator(Kenkyū-buntansha) |
伊藤 昭博 国立研究開発法人理化学研究所, その他, 研究員 (40391859)
藤井 渉 東京大学, 大学院農学生命科学研究科(農学部), 助教 (40708161)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAHASHI Shinichiro 東京大学, 大学院農学生命科学研究科, 准教授 (00197146)
SHIOYA Ryuji 東洋大学, 総合情報学部, 教授 (70282689)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | アミノ酸代謝 / 脂肪合成 / 脂肪肝 / 筋脂肪交雑 / インスリン / インスリン様成長因子 / 脂質代謝 / アミノ酸シグナル / 機械学習 / 人工知能 / 栄養学 / 応用動物 / 畜産学 / アミノ酸欠乏食 / 臓器特異的脂肪蓄積 / IGF / mTOR / 生理活性 / シグナル伝達 / アミノ酸栄養 |
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| Outline of Final Research Achievements |
The present study was undertaken to evaluate the mechanism underlying that amino acid deficient diet regulates the lipid metabolism. Here we showed that Arg deficient diet induced liver Triacylglycerol (TG) accumulation by inhibiting lipid secretion from the liver. Low-amino acid diet accumulated liver TG by promoting lipid synthesis in liver. On the other hand, Lys deficient diet induced TG accumulation in muscle through induction of slow-muscle formation. By using machine learning method, relation between serum amino acid level and liver TG level was analyzed. Self-Organizing-Mapping (SOM) method could classify the TG level by using only serum amino acid level. Multiperceptron (MLP) analysis could predict the liver TG level from only serum amino acid level. These data showed that serum amino-acid profile could define liver TG level.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] USP15 attenuates IGF-I signaling by antagonizing Nedd4-induced IRS-2 ubiquitination.2017
Author(s)
Fukushima T, Yoshihara H, Furuta H, Hakuno F, Iemura SI, Natsume T, Nakatsu Y, Kamata H, Asano T, Komada M, Takahashi SI.
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 484
Issue: 3
Pages: 522-528
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The Novel Functions of High-Molecular-Mass Complexes Containing Insulin Receptor Substrates in Mediation and Modulation of Insulin-Like Activities: Emerging Concept of Diverse Functions by IRS-Associated Proteins.2015
Author(s)
Hakuno F, Fukushima T, Yoneyama Y, Kamei H, Ozoe A, Yoshihara H, Yamanaka D, Shibano T, Sone-Yonezawa M, Yu BC, Chida K, Takahashi S.
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Journal Title
Front Endocrinol (Lausanne).
Volume: 6
Pages: 73-73
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Nedd4-induced mono-ubiquitination of IRS-2 enhances IGF signaling and mitogenic activity.2015
Author(s)
Toshiaki Fukushima, Hidehito Yoshihara, Haruka Furuta, Fumihiko Hakuno, Jing Luan, Cunming Duan, Yasushi Saeki, Keiji Tanaka, Shun-Ichiro Iemura, TohruNatsume, Kazuhiro Chida, Yusuke Nakatsu, Hideaki Kamata, Tomoichiro Asano, and Shin-Ichiro Takahashi
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Journal Title
Nature communications
Volume: 6
Issue: 1
Pages: 6780-6780
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] Lysine deficiency signal selectively induces lipid accumulation in muscle and adipose tissues of rats.2017
Author(s)
Yuki Goda, Hiroki Nishi, Mikako Kumano, Daisuke Yamanaka, Hiroyasu Kamei, Keitaro Yamanouchi, Masaya Katsumata, Hisanori Kato, Kazuhiro Chida, Takahashi SI
Organizer
Gordon Research Conference
Place of Presentation
Ventura, CA, USA
Year and Date
2017-03-12
Related Report
Int'l Joint Research
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[Presentation] Amino acid-deficiency signal induces insulin-like effects on gluconeogenic gene regulation independently of insulin signaling in hepatocytes.2017
Author(s)
Mikako Kumano, Hiroki Nishi, Daisuke Yamanaka, Hiroyasu Kamei, Yuki Goda, Yuka Toyoshima, Asako Takenaka, Kazuhiro Chida, Fumihiko Hakuno, Shin-Ichiro Takahashi.
Organizer
Gordon Research Conference
Place of Presentation
Ventura, CA, USA
Year and Date
2017-03-12
Related Report
Int'l Joint Research
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[Presentation] Hepatic steatosis induced by amino acid deficiency or by manipulation of the dietary amino acid composition.2017
Author(s)
○Nishi, Hiroki, Daisuke Yamanaka1, Hiroyasu Kamei, Yuki Goda, Mikako Kumano, Yuka Toyoshima, Asako Takenaka, Masato Masuda, Ryuji Shioya, Kazuhiro Chida, Fumihiko Hakuno, Shin-Ichiro Takahashi
Organizer
Gordon Research Conference
Place of Presentation
Ventura, CA, USA
Year and Date
2017-03-12
Related Report
Int'l Joint Research
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[Presentation] Lysine deficiency signal selectively induces lipid accumulation in muscle and adipose tissues of rats.2017
Author(s)
Yuki Goda, Hiroki Nishi, Mikako Kumano, Daisuke Yamanaka, Hiroyasu Kamei, Keitaro Yamanouchi, Masaya Katsumata, Hisanori Kato, Kazuhiro Chida, Masugi Nishihara, Fumihiko Hakuno, Shin-Ichiro Takahashi
Organizer
国際栄養学会
Related Report
Int'l Joint Research
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[Presentation] Actin cytoskeleton remodeling dynmics of adherent cells under to the photo-induced mechanical strain of gelatin substrate2016
Author(s)
Kwokhoi Ng, Kentaro Iketaki, Ryuzo Kawamura, Seiichiro Nakabayashi, Yosuke Yoneyama, Fumihiko Hakuno, Shin-Ichiro Takahashi, Fumiki Yanagawa, Toshiyuki Takagi, Shinji Sugiura, Toshiyuki Kawamori, Hiroshi Yoshikawa
Organizer
日本生物物理学会
Place of Presentation
つくば国際会議場（茨城、つくば）
Year and Date
2016-11-25
Related Report
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