Role of calcium oscillations in embryo implantation and pregnancy in mammals

• Project/Area Number: 15H04584
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal production science
• Research Institution: Azabu University
• Principal Investigator: Ito Junya 麻布大学, 獣医学部, 准教授 (30454143)
• Co-Investigator(Kenkyū-buntansha): 柏崎 直巳 麻布大学, 獣医学部, 教授 (90298232)
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 受精 / 着床 / 妊娠 / カルシウム / ブタ / マウス / 発生 / 胚着床 / 胚

Outline of Final Research Achievements

In mammals, developmental ability of in vitro produced embryos is low because the molecular mechanism of fertilization still remains unclear in mammals.In this study, we focused on the oocyte-activating factor derived from sperm. Our results showed phospholipase C zeta (PLCz) has an important role in inducing calicium oscillations in the mouse. In addtion, we identified PLCz has species dependent activity. Our results also showed zinc signaling as well as calcium signal has an essential role in mammalian fertilziation.

Academic Significance and Societal Importance of the Research Achievements

これまで研究されてきたすべての動物種において，受精時には卵内カルシウムの上昇が認められることから哺乳類においても受精メカニズムはカルシウムシグナルを中心に論じられてきた．本研究成果により．哺乳類の受精時におけるカルシウム上昇には卵活性化因子(PLCz)が重要なこと，また哺乳類動物種によってPLCzの活性は異なることが明らかとなった．さらにカルシウムシグナルと同様に亜鉛シグナルも哺乳類の受精において重要な役割を持つことが明らかとなった．

Research Products

• [Journal Article] Molecular mechanisms of embryonic implantation in mammals: Lessons from the gene manipulation of mice (2018)
  • Author(s): Namiki Takafumi、Ito Junya、Kashiwazaki Naomi
  • Journal Title: Reproductive Medicine and Biology Volume: 印刷中 Issue: 4 Pages: 331-342
  • DOI: 10.1002/rmb2.12103
  • Peer Reviewed / Open Access
• [Journal Article] Application of auxin-inducible degron technology to mouse oocyte activation with PLCζ (2018)
  • Author(s): 1.Miura K, Matoba S, Ogonuki N, Namiki T, Ito J, Kashiwazaki N, Ogura A
  • Journal Title: Journal of Reproduction and Development Volume: 64 Issue: 4 Pages: 319-326
  • DOI: 10.1262/jrd.2018-053
  • NAID: 130007437366
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] Generation of rats from vitrified oocytes with surrounding cumulus cells via in vitro fertilization with cryopreserved sperm (2017)
  • Author(s): Fujiwara K, Kamoshita M, Kato T, Ito J, Kashiwazaki N.
  • Journal Title: Animal Science Journal Volume: 88 Issue: 1 Pages: 180-184
  • DOI: 10.1111/asj.12666
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 不妊症の現状とその克服に向けた亜鉛シグナルの解明 (2019)
  • Author(s): 伊藤潤哉
  • Organizer: 第18回亜鉛栄養治療研究会
  • Invited
• [Presentation] Expression of zinc transporters in germ cells and reproductive tissues in mice (2017)
  • Author(s): Junya ITO, Takafumi Namiki, Midori Ishii, Maki Kamoshita, Naomi Kashiwazaki
  • Organizer: Gordon Research Conference
  • Int'l Joint Research
• [Presentation] 哺乳類の受精時における二価イオンの役割 ～Ca2+オシレーション vs Zn2+スパーク～ (2017)
  • Author(s): 伊藤潤哉
  • Organizer: 第35回日本受精着床学会総会・学術講演会
  • Invited