Role of calcium oscillations in embryo implantation and pregnancy in mammals
| Project/Area Number |
15H04584
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | Azabu University |
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Principal Investigator |
Ito Junya 麻布大学, 獣医学部, 准教授 (30454143)
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| Co-Investigator(Kenkyū-buntansha) |
柏崎 直巳 麻布大学, 獣医学部, 教授 (90298232)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | 受精 / 着床 / 妊娠 / カルシウム / ブタ / マウス / 発生 / 胚着床 / 胚 |
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| Outline of Final Research Achievements |
In mammals, developmental ability of in vitro produced embryos is low because the molecular mechanism of fertilization still remains unclear in mammals.In this study, we focused on the oocyte-activating factor derived from sperm. Our results showed phospholipase C zeta (PLCz) has an important role in inducing calicium oscillations in the mouse. In addtion, we identified PLCz has species dependent activity. Our results also showed zinc signaling as well as calcium signal has an essential role in mammalian fertilziation.
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| Academic Significance and Societal Importance of the Research Achievements |
これまで研究されてきたすべての動物種において，受精時には卵内カルシウムの上昇が認められることから哺乳類においても受精メカニズムはカルシウムシグナルを中心に論じられてきた．本研究成果により．哺乳類の受精時におけるカルシウム上昇には卵活性化因子(PLCz)が重要なこと，また哺乳類動物種によってPLCzの活性は異なることが明らかとなった．さらにカルシウムシグナルと同様に亜鉛シグナルも哺乳類の受精において重要な役割を持つことが明らかとなった．
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Report
(5 results)
Research Products
(6 results)
