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Identification of Neospora virulence factor and its application for the vaccine development

Research Project

Project/Area Number 15H04589
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Veterinary medical science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

Nishikawa Yoshifumi  帯広畜産大学, 原虫病研究センター, 教授 (90431395)

Co-Investigator(Renkei-kenkyūsha) IGARASHI Makoto  帯広畜産大学, 原虫病研究センター, 教授 (60374766)
KURODA Yasuhiro  東海大学, 工学部, 准教授 (40398756)
Research Collaborator IHARA Fumiaki  
Ragab Fereig  
Arpron Leesombun  
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords感染症 / ワクチン / 免疫 / 畜産 / ネオスポラ / 免疫学
Outline of Final Research Achievements

In this study, we aimed at identifying virulence factors of Neoospora caninum, which induces reproductive disorders in livestock, and its application for the vaccine development. Because excess inflammatory response may be involved in pathogenicity, screening of Neospora molecules activating host signaling of immune responses was performed. The activating factor-deficient parasites lost the virulence in mice. Furthermore, vaccines based on the activating factor induced protective immune responses. With no effective drugs or vaccines available to control neosporosis, our results will be valuable for the future vaccine development.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (25 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (6 results) (of which Invited: 1 results) Book (2 results) Remarks (5 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] South Valley University(エジプト)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] マヒドン大学(タイ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of the Philippines Cebu(フィリピン)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] A clinical case of neosporosis in a 4-week-old holstein friesian calf which developed hindlimb paresis postnatally2018

    • Author(s)
      Uesaka, K., Koyama, K., Horiuchi, N., Kobayashi, Y., Nishikawa, Y. and Inokuma H.
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 80 Issue: 2 Pages: 280-283

    • DOI

      10.1292/jvms.17-0205

    • NAID

      130006386447

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of anti-Neospora caninum antibody in serum and cerebrospinal fluid from a calf with neosporosis2017

    • Author(s)
      Karin UESAKA, Hisashi INOKUMA, Noriyuki HORIUCHI, Yoshiyasu KOBAYASHI and Hidefumi FURUOKA, Yoshifumi NISHIKAWA
    • Journal Title

      Japanese Journal of Veterinary Parasitology

      Volume: 16 Pages: 18-21

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Immunization with Toxoplasma gondii peroxiredoxin 1 induces protective immunity against toxoplasmosis in mice2017

    • Author(s)
      Fereig Ragab M.、Kuroda Yasuhiro、Terkawi Mohamad Alaa、Mahmoud Motamed Elsayed、Nishikawa Yoshifumi
    • Journal Title

      Plos One

      Volume: 12 Issue: 4 Pages: e0176324-e0176324

    • DOI

      10.1371/journal.pone.0176324

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Towards a preventive strategy for neosporosis: challenges and future perspectives for vaccine development against infection with <i>Neospora caninum</i>2017

    • Author(s)
      Nishikawa Y
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 79 Issue: 8 Pages: 1374-1380

    • DOI

      10.1292/jvms.17-0285

    • NAID

      130005950130

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of Thai piperaceae plant extracts on Neospora caninum infection2017

    • Author(s)
      Leesombun A, Boonmasawai S, Nishikawa Y
    • Journal Title

      Parasitol Int.

      Volume: 66 Issue: 3 Pages: 219-226

    • DOI

      10.1016/j.parint.2017.01.017

    • NAID

      120006390999

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Peroxiredoxin 3 promotes IL-12 production from macrophages and partially protects mice against infection with Toxoplasma gondii2016

    • Author(s)
      Fereig RM, Nishikawa Y
    • Journal Title

      Parasitol Int.

      Volume: 65 Issue: 6 Pages: 741-748

    • DOI

      10.1016/j.parint.2016.09.008

    • NAID

      120006390971

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] In vitro and in vivo effects of the phytohormone inhibitor fluridone against Neospora caninum infection2016

    • Author(s)
      ba&#241;ez, R.H., Leesombun, A., Nishimura, M., Matsubara, R., Kojima, M., Sakakibara, H., Nagamune, K., Nishikawa, Y.
    • Journal Title

      Parasitol. Int.

      Volume: 65 Issue: 4 Pages: 319-322

    • DOI

      10.1016/j.parint.2016.03.009

    • NAID

      120006390951

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Changes in neurotransmitter levels and expression of immediate early genes in brain of mice infected with Neospora caninum.2016

    • Author(s)
      Ihara F, Nishimura M, Muroi Y, Furuoka H, Yokoyama N, Nishikawa Y
    • Journal Title

      Sci Rep.

      Volume: 6:23052 Issue: 1 Pages: 1-9

    • DOI

      10.1038/srep23052

    • NAID

      120006390936

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] ネオスポラ症の病態発生機序と防御法に関する研究2016

    • Author(s)
      西川義文
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      日本大学(神奈川県・藤沢市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 牛の血清および脳脊髄液中ネオスポラ抗体の診断的意義の検討2016

    • Author(s)
      上坂花鈴、西川義文、猪熊 壽
    • Organizer
      第159回日本獣医学会学術集会
    • Place of Presentation
      日本大学(神奈川県・藤沢市)
    • Year and Date
      2016-09-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] 後躯麻痺を呈した4週齢のホルスタイン種子牛におけるネオスポラ症の1症例2015

    • Author(s)
      猪熊 壽、中島永成、岩澤裕介、堀内雅之、古林与志安、西川義文
    • Organizer
      第46回日本家畜臨床学会学術集会
    • Place of Presentation
      秋田温泉・さとみ(秋田県・秋田市)
    • Year and Date
      2015-11-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] トキソプラズマおよびネオスポラに対するタイ産コショウ科植物由来エタノール抽出物の抗原虫効果2015

    • Author(s)
      Arpron Leesombun、Sookruetai Boonmasawai、西川義文
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      北里大学・獣医学部(青森県・十和田市)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Transcriptome and Histopathological Changes in Mouse Brain Infected with Neospora caninum2015

    • Author(s)
      西村麻紀、田中沙智、猪原史成、室井喜景、山岸潤也、古岡秀文、鈴木穣、西川義文
    • Organizer
      第158回日本獣医学会学術集会
    • Place of Presentation
      北里大学・獣医学部(青森県・十和田市)
    • Year and Date
      2015-09-07
    • Related Report
      2015 Annual Research Report
  • [Presentation] Vaccine efficacy of oligomannose-coated liposomes against Toxoplasma and Plasmodium infection.2015

    • Author(s)
      Yoshifumi Nishikawa, Alaa Terkawi, Sachi Tanaka, Yasuhiro Kuroda, Naoya Kojima
    • Organizer
      第25回 World Association for the Advancement of Veterinary Parasitology
    • Place of Presentation
      The Arena and Convention Centre(イギリス・リバプール)
    • Year and Date
      2015-08-17
    • Related Report
      2015 Annual Research Report
  • [Book] 寄生虫病学2017

    • Author(s)
      西川義文
    • Publisher
      緑書房
    • Related Report
      2016 Annual Research Report
  • [Book] Towards a Preventive Strategy for Toxoplasmosis: Current Trends, Challenges, and Future Perspectives for Vaccine Development, Sunil Thomas (ed.), Vaccine Design: Methods and Protocols, Volume 2: Vaccines for Veterinary Diseases, Methods in Molecular Biology2016

    • Author(s)
      Fereig RM, Nishikawa Y
    • Total Pages
      12
    • Publisher
      Springer Science
    • Related Report
      2015 Annual Research Report
  • [Remarks] 西川研究室

    • URL

      https://sites.google.com/site/nishihdlab/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 原虫病研究センター

    • URL

      http://www.obihiro.ac.jp/~protozoa/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 原虫病研究センターの西川義文准教授が「2016-2017年度日本獣医学会賞」を受賞

    • URL

      http://www.obihiro.ac.jp/topic/2016/nishikawajyusyou_28.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 原虫病研究センター・感染免疫部門・生体防御学分野・西川義文研究室が研究成果を発表しました。

    • URL

      http://www.obihiro.ac.jp/~protozoa/2016gyoseki-topics.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 平成28年3月:論文発表

    • URL

      https://sites.google.com/site/nishihdlab/home/news

    • Related Report
      2015 Annual Research Report
  • [Patent(Industrial Property Rights)] アジュバント2017

    • Inventor(s)
      西川義文、ラガブ マッハルーフ マハムッド フェレイク
    • Industrial Property Rights Holder
      西川義文、ラガブ マッハルーフ マハムッド フェレイク
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-252032
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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