The role of canine AIM in histiocytic sarcoma

• Project/Area Number: 15H04590
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: The University of Tokyo
• Principal Investigator: Yonezawa Tomohiro 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (10433715)
• Co-Investigator(Kenkyū-buntansha): 松木 直章 東京大学, 大学院農学生命科学研究科(農学部), 教授 (40251417) ; 盆子原 誠 日本獣医生命科学大学, 獣医学部, 教授 (50343611)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: マクロファージ / 組織球性肉腫 / 犬 / イヌ / 腫瘍 / ヌードマウス / アポトーシス / 獣医学 / 癌 / 免疫 / がん / AIM / CD5L

Outline of Final Research Achievements

Histiocytic sarcoma, macrophage-derived tumor is seriously malignant and therapeutic method is not established. In this study, we focused on apoptosis inhibitor of macrophage (AIM). It is found that AIM is expressed in several normal tissues and histiocytic tumors. When AIM expression vector was transfected into histiocytic cell lines, the cell number was reduced. Canine recombinant AIM also could induce apoptosis of these cells. It is suggested canine AIM has apoptotic inducing effect in histiocytic sarcoma.

Research Products

• [Int'l Joint Research] Moffitt cancer center(米国)
• [Journal Article] Decreased plasma amino acid concentrations in cats with chronic gastrointestinal diseases and their possible contribution in the inflammatory response. (2018)
  • Author(s): Sakai K, Maeda S, Yonezawa T, Matsuki N.
  • Journal Title: Vet Immunol Immunopathol. Volume: 195 Pages: 1-6
  • DOI: 10.1016/j.vetimm.2017.11.001
  • Peer Reviewed
• [Journal Article] Presence of contagious yawning in sheep (2017)
  • Author(s): Yonezawa Tomohiro、Sato Kohei、Uchida Mona、Matsuki Naoaki、Yamazaki Atusi
  • Journal Title: Animal Science Journal Volume: 88 Issue: 1 Pages: 195-200
  • DOI: 10.1111/asj.12681
  • Peer Reviewed / Open Access
• [Journal Article] Fecal microbiome in dogs with inflammatory bowel disease and intestinal lymphoma (2017)
  • Author(s): Omori M, Maeda S, Igarashi H, Ohno K, Sakai K, Yonezawa T, Horigome A, Odamaki T, Matsuki N
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Issue: 11 Pages: 1840-1847
  • DOI: 10.1292/jvms.17-0045
  • NAID: 130006206562
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Density of tumor-infiltrating granzyme B-positive cells predicts favorable prognosis in dogs with transitional cell carcinoma (2017)
  • Author(s): Inoue Akiko、Maeda Shingo、Kinoshita Ryohei、Tsuboi Masaya、Yonezawa Tomohiro、Matsuki Naoaki
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 190 Pages: 53-56
  • DOI: 10.1016/j.vetimm.2017.07.001
  • Peer Reviewed
• [Journal Article] Endoscopic Cytology for the Diagnosis of Chronic Enteritis and Intestinal Lymphoma in Dogs (2017)
  • Author(s): Maeda S.、Tsuboi M.、Sakai K.、Ohno K.、Fukushima K.、Kanemoto H.、Hiyoshi-Kanemoto S.、Goto-Koshino Y.、Chambers J. K.、Yonezawa T.、Uchida K.、Matsuki N.
  • Journal Title: Veterinary Pathology Volume: 54 Issue: 4 Pages: 595-604
  • DOI: 10.1177/0300985817705175
  • Peer Reviewed
• [Journal Article] Cycle Influences Spontaneous Delivery in Cows. (2016)
  • Author(s): Yonezawa T, Uchida M, Tomioka M, Matsuki N
  • Journal Title: PLoS One Volume: 11(8) Issue: 8 Pages: e0161735-e0161735
  • DOI: 10.1371/journal.pone.0161735
  • Peer Reviewed / Open Access
• [Journal Article] Apoptosis inhibitor of macrophage (AIM) reduces cell number in canine histiocytic sarcoma cell lines (2016)
  • Author(s): Uchida M, Saeki K, Maeda S, Tamahara S, Yonezawa T, Matsuki N
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 9 Pages: 1515-1520
  • DOI: 10.1292/jvms.16-0120
  • NAID: 130005598036
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prolactin suppression of Gonadotropin releasing hormone initiation of mammary gland involution in female rats. (2016)
  • Author(s): Rieanrakwong D, Laoharatchatathanin T, Terashima R, Yonezawa T, Kurusu S, Hasegawa Y, Kawaminami M.
  • Journal Title: Endocrinol Volume: 157(7) Issue: 7 Pages: 2750-2758
  • DOI: 10.1210/en.2016-1180
  • Peer Reviewed / Open Access
• [Journal Article] Synovial fluid matrix metalloproteinase-2 and -9 activities in dogs suffering from joint disorders (2016)
  • Author(s): Murakami K, Maeda S, Yonezawa T, Matsuki N
  • Journal Title: Journal of Veterinary Medical Science Volume: 78 Issue: 6 Pages: 1051-1054
  • DOI: 10.1292/jvms.15-0711
  • NAID: 130005159665
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Gonadotropin-releasing hormone is prerequisite for the constitutive expression of pituitary annexin A5 (2015)
  • Author(s): Yonezawa T, Watanabe A, Kurusu S, Kawaminami M.
  • Journal Title: Endocrine Journal Volume: 62 Issue: 12 Pages: 1127-1132
  • DOI: 10.1507/endocrj.EJ15-0410
  • NAID: 130005115210
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Anti-metastatic outcome of isoform-specific prolactin receptor targeting in breast cancer. (2015)
  • Author(s): Yonezawa T, Chen KH, Ghosh MK, Rivera L, Dill R, Ma L, Villa PA, Kawaminami M, Walker AM
  • Journal Title: Cancer Lett Volume: 366 Issue: 1 Pages: 84-92
  • DOI: 10.1016/j.canlet.2015.06.010
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Augmentation of Metastin/Kisspeptin mRNA Expression by the Proestrous Luteinizing Hormone Surge in Granulosa Cells of Rats: Implications for Luteinization. (2015)
  • Author(s): Laoharatchatathanin T, Terashima R, Yonezawa T, Kurusu S, Kawaminami M.
  • Journal Title: Biol Reprod Volume: 93 Issue: 1 Pages: 1-9
  • DOI: 10.1095/biolreprod.115.127902
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Alteration of somatostatin receptor 2 expression in canine mammary gland tumor (2015)
  • Author(s): Sakai K, Yonezawa T, Yamawaki H, Oyamada T.
  • Journal Title: Journal of Veterinary Medical Science Volume: 77 Issue: 10 Pages: 1319-1322
  • DOI: 10.1292/jvms.15-0002
  • NAID: 130005107591
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Syngeneic ANXA5 inhibition of mammary tumor cell growth in the dog (2017)
  • Author(s): Mona Uchida, Kohei Saeki, Shingo Maeda, Tomohiro Yonezawa, Naoaki Matsuki and Mitsumori Kawaminami
  • Organizer: Annexin Conference 2017
  • Int'l Joint Research
• [Presentation] Relationship between lunar cycle and spontaneous delivery date in Holstein cows (2017)
  • Author(s): Tomohiro Yonezawa, Dai Ishiyama, Mona Uchida, Michiko Tomioka, Kazunori Ishii, Shizuko Maeda, Yoji Sasai, Masatsugu Asada, Tadashi Kawamura, Yoshihiro Nakamura, Eimei Sato, Shingo Maeda, Naoaki Matsuki
  • Organizer: WCRB2017
  • Int'l Joint Research
• [Presentation] Down-regulation of Annexin A5 (ANXA5) expression in canine mammary tumor cells and a possibility of therapeutic application of ANXA5 (2015)
  • Author(s): Mona Uchida, Kosei Sakai, Tomohiro Yonezawa, Mitsumori Kawaminami
  • Organizer: Annexin Conference 2015
  • Place of Presentation: Maastricht, Netherland
  • Year and Date: 2015-09-08
  • Int'l Joint Research
• [Presentation] Apoptosis inhibitor of macrophage in dog: molecular characterization and inhibitory effect on histiocytic sarcoma cell proliferation in vitro (2015)
  • Author(s): Mona Uchida, Shintaro Tomura, Kohei Saeki, Shingo Maeda, Satoshi Tamahara, Tomohiro Yonezawa, Naoaki Matsuki
  • Organizer: International Conference of Cancer Immunotherapy and Macrophages 2015
  • Place of Presentation: 東京大学
  • Year and Date: 2015-07-09
  • Int'l Joint Research
• [Presentation] Apoptosis inhibitor of macrophage (AIM)がイヌ組織球性肉腫株化細胞にもたらす影響 (2015)
  • Author(s): 内田萌菜、米澤智洋、佐伯亘平、盆子原誠、前田真吾、松木直章
  • Organizer: 獣医臨床病理学会2015年大会
  • Place of Presentation: 日本獣医生命科学大学
  • Year and Date: 2015-05-30
• [Presentation] イヌ乳腺腫瘍におけるアネキシンA5（Anxa5）の発現 (2015)
  • Author(s): 内田萌菜、酒居幸生、米澤智洋、汾陽光盛
  • Organizer: 第１回日本アネキシン研究会
  • Place of Presentation: 東京大学
• [Patent(Industrial Property Rights)] 腫瘍の治療剤およびその使用 (2016)
  • Inventor(s): 米澤智洋、内田萌菜、松木直章、金村優香、川上和夫
  • Industrial Property Rights Holder: 米澤智洋、内田萌菜、松木直章、金村優香、川上和夫
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-05-26
• [Patent(Industrial Property Rights)] Therapeutic agent for tumor derived from immune cells and use thereof (2015)
  • Inventor(s): Tomohiro Yonezawa et al
  • Industrial Property Rights Holder: Tomohiro Yonezawa et al
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-05-28
  • Overseas