| Project/Area Number |
15H04592
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
高須 正規 岐阜大学, 応用生物科学部, 准教授 (00503327)
大屋 賢司 岐阜大学, 応用生物科学部, 准教授 (50402219)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | トキソプラズマ / 流産 / 胎盤 / 血管内皮 / 垂直感染 / 人獣共通感染症 / 感染 / メチル化 / バリア機能 |
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| Outline of Final Research Achievements |
We analyzed the expression pattern of adhesion molecules at the border of maternal blood flow and fetus derived cells in the placenta and showed that the expression of some adhesion molecules was suppressed. Furthermore, when the behavior of infected leukocytes in the bloodstream was visualized, in some organs, infected leukocytes tended to adhere to the vascular endothelium more easily than non-sensitized leukocytes, and tended to remain efficiently in the organs. In addition, the protozoa escaped from the leukocytes within 4 hours from the inside of the infected leukocytes attached to the vascular endothelium. On the other hand, it was confirmed that such a phenomenon did not occur on the surface of trophoblast cells in contact with the maternal blood flow in the placenta.
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| Academic Significance and Societal Importance of the Research Achievements |
母体から胎児への感染が獣医・医学上の問題となる病原体は多くが細胞内寄生性である。このような病原体は細胞内部に侵入できるため、細胞間接着や融合によって形成される「形態的」血液胎盤関門は無力である。従来の基礎研究はこのような「形態的バリア」に関するものがほとんどであったため、成果が母子垂直感染現象の理解につながらなかった。これに対し本研究では細胞内寄生性病原体をも防ぎうる「機能的バリア」という新概念について研究した。この新概念のバリア機構について得られた知見は、母子感染の制圧に資する重要な情報となり得る。
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