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Species-dependent neural function organized by non-coding RNAs

Research Project

Project/Area Number 15H04603
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Integrative animal science
Research InstitutionKyushu University

Principal Investigator

IMAMURA TAKUYA  九州大学, 医学研究院, 准教授 (90390682)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Keywords種特異的 / 転写因子 / ノンコーディングRNA / DNA脱メチル化 / 神経幹細胞 / エピジェネティクス / 進化 / エピゲノム / ノンコーディング / 霊長類 / 生物多様性
Outline of Final Research Achievements

Vast majority of the fundamental research projects on the mammalian brain utilize the mouse systems. However, morphological and functional differences between primates and rodents evidently exist, and therefore, the molecules that underlie the generation of the differences need be identified. In this study, focusing on the promoter-associated non-coding RNAs (pancRNAs), the comprehensive databases have been made for 5 different mammalian species. Now functional pancRNAs are being screened, that potentially differentiate the neural stem cell behaviors depending on species.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (38 results)

All 2018 2017 2016 2015 Other

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (26 results) (of which Int'l Joint Research: 11 results,  Invited: 2 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Epigenetic regulation of neural stem cell differentiation towards spinal cord regeneration2018

    • Author(s)
      Kameda Tomonori、Imamura Takuya、Nakashima Kinichi
    • Journal Title

      Cell and Tissue Research

      Volume: 371 Issue: 1 Pages: 189-199

    • DOI

      10.1007/s00441-017-2656-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA Methylome Analysis Identifies Transcription Factor-Based Epigenomic Signatures of Multilineage Competence in Neural Stem/Progenitor Cells.2017

    • Author(s)
      Sanosaka T, Imamura T, Hamazaki N, Chai M, Igarashi K, Ideta-Otsuka M, Miura F, Ito T, Fujii N, Ikeo K, Nakashima K
    • Journal Title

      Cell Rep

      Volume: 20 Issue: 12 Pages: 2992-3003

    • DOI

      10.1016/j.celrep.2017.08.086

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of Bidirectional Promoter-Derived lncRNAs from Small-Scale Samples Using Pre-Amplification-Free Directional RNA-seq Method2017

    • Author(s)
      Hamazaki Nobuhiko、Nakashima Kinichi、Hayashi Katsuhiko、Imamura Takuya
    • Journal Title

      Methods in Molecular Biology

      Volume: 1605 Pages: 83-103

    • DOI

      10.1007/978-1-4939-6988-3_6

    • ISBN
      9781493969869, 9781493969883
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolutionary acquisition of promoter-associated non-coding RNA (pancRNA) repertoires diversifies species-dependent gene activation mechanisms in mammals.2017

    • Author(s)
      Masahiro Uesaka, Kiyokazu Agata, Takao Oishi, Kinichi Nakashima, Takuya Imamura
    • Journal Title

      BMC Genomics

      Volume: 18 Issue: 1 Pages: 285-285

    • DOI

      10.1186/s12864-017-3662-1

    • NAID

      120006305760

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Manipulation of Promoter-Associated Noncoding RNAs in Mouse Early Embryos for Controlling Sequence-Specific Epigenetic Status.2017

    • Author(s)
      Hamazaki N, Nakashima K, Imamura T.
    • Journal Title

      Methods Mol Biol

      Volume: 1543 Pages: 271-282

    • DOI

      10.1007/978-1-4939-6716-2_16

    • ISBN
      9781493967148, 9781493967162
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstitution in vitro of the entire cycle of the mouse female germ line.2016

    • Author(s)
      Hikabe O, Hamazaki N, Nagamatsu G, Obata Y, Hirao Y, Hamada N, Shimamoto S, Imamura T, Nakashima K, Saitou M, Hayashi K
    • Journal Title

      Nature

      Volume: 539 Issue: 7628 Pages: 299-303

    • DOI

      10.1038/nature20104

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Bidirectional promoters link cAMP signaling with irreversible differentiation through promoter-associated noncoding RNA (pancRNA) expression in PC12 cells.2016

    • Author(s)
      Naoki Yamamoto, Kiyokazu Agata, Kinichi Nakashima, Takuya Imamura
    • Journal Title

      Nucleic Acids Research

      Volume: in press Issue: 11 Pages: 5105-5122

    • DOI

      10.1093/nar/gkw113

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 長鎖ncRNAによるほ乳類エピゲノム制御2017

    • Author(s)
      今村拓也
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Regulation of non-coding RNA contributes to the complete cessation of cell proliferation of neuron-like cells2017

    • Author(s)
      Takuya IMAMURA
    • Organizer
      France Japan Epigenetics Workshop 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ニューロンにおけるエピゲノム制御とその破綻2017

    • Author(s)
      今村拓也
    • Organizer
      第44回日本毒性学会学術大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] DNA methylomes identifiy transcription factor-based epigenomic signatures for timed acquisition of differentiation competence in neural stem/progenitor cells towards neuronal and glia lineages2017

    • Author(s)
      今村拓也、佐野坂司、浜崎伸彦、Chai Muh Chyi、五十嵐勝秀、大塚まき、三浦史仁、伊藤隆司、藤井信之、池尾一穂、中島欽一
    • Organizer
      4th World Congress of Reproductive Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DNA methylome analysis identifies transcription factor-based epigenomic signatures of multi-lineage competence in neural stem/progenitor cells2017

    • Author(s)
      今村拓也、佐野坂司、浜崎伸彦、Chai Muh Chyi、五十嵐勝秀、大塚まき、三浦史仁、伊藤隆司、藤井信之、池尾一穂、中島欽一
    • Organizer
      The 72nd Fujihara Seminar Molecular Mechanism of Molding and Disruption of the Epigenomes Underlying Cellular Community
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mechanism underlying developmental stage dependent changes in neural stem cells responsiveness to Bone Morphogenetic Proteins2017

    • Author(s)
      本田瑞季、堅田明子、大塚まき、山本直樹、五十嵐勝秀、今村拓也、中島欽一
    • Organizer
      The 72nd Fujihara Seminar Molecular Mechanism of Molding and Disruption of the Epigenomes Underlying Cellular Community
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Neuronal activity-dependent DNA methylation changes in the naive hippocampal neurons accelerate gene expression responses to the following stimuli2017

    • Author(s)
      亀田朋典、今村拓也、滝沢琢己、三浦史仁、伊藤隆司、中島欽一
    • Organizer
      The 72nd Fujihara Seminar Molecular Mechanism of Molding and Disruption of the Epigenomes Underlying Cellular Community
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of non-coding RNA contributes to the complete cessation of cell proliferation of neuron-like cells2017

    • Author(s)
      今村拓也、山本直樹、阿形清和、中島欽一
    • Organizer
      第43回内藤コンファレンス
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス海馬ニューロンは神経活動依存的にDNAメチロームを変動し、脱メチル化を介して遺伝子発現応答を高速化する2017

    • Author(s)
      亀田朋典、今村拓也、滝沢琢己、三浦史仁、伊藤隆司、中島欽一
    • Organizer
      第11回日本エピジェネティクス研究会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒトES/iPS細胞から神経幹細胞への誘導とその分化過程におけるノンコーディングRNA(pancRNA)を介した特異的遺伝子活性化2016

    • Author(s)
      藤本雄一、亀田朋典、小野田孝太、吉良潤一、中島欽一、今村拓也
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス海馬ニューロンにおいて神経活動によりDNA化が変動し遺伝子発現応答能を改変する2016

    • Author(s)
      亀田朋典、今村拓也、滝沢琢己、木村文香、三浦史仁、伊藤隆司、中島欽一
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Regulation of non-coding RNA contributes to the complete cessation of. cell proliferation of neuron-like cells2016

    • Author(s)
      Takuya Imamura, Naoki Yamamoto, Kiyokazu Agata, Kinichi Nakashima
    • Organizer
      Society for Neuroscience 2016
    • Place of Presentation
      San Diego Convention Center (San Diego, CA, USA)
    • Year and Date
      2016-11-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 長鎖ノンコーディングRNAはほ乳類ニューロン完全増殖停止に必須である2016

    • Author(s)
      山本直樹、阿形清和、中島欽一、今村拓也
    • Organizer
      第109回日本繁殖生物学会大会
    • Place of Presentation
      麻布大学(神奈川)
    • Year and Date
      2016-09-11
    • Related Report
      2016 Annual Research Report
  • [Presentation] Regulation of Non-coding RNA Contributes to the Complete Cessation of Cell Proliferation of Neuron-like Cells2016

    • Author(s)
      山本直樹、阿形清和、中島欽一、今村拓也
    • Organizer
      第35回札幌国際がんシンポジウム
    • Place of Presentation
      ロイトン札幌(北海道)
    • Year and Date
      2016-06-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス胎仔海馬由来初代培養ニューロンにおける神経活動依存的DNAメチローム変動の検出2016

    • Author(s)
      亀田朋典、今村拓也、滝沢琢己、木村文香、三浦史仁、伊藤隆司、中島欽一
    • Organizer
      第35回札幌国際がんシンポジウム
    • Place of Presentation
      ロイトン札幌(北海道)
    • Year and Date
      2016-06-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マウス性的二型核内の細胞でみられるアンドロジェン依存的なDNAメチル化レベルの変化2016

    • Author(s)
      小野田孝太、佐藤弘明、浜崎伸彦、中嶋秀行、束村博子、前多敬一郎、中島欽一、今村拓也
    • Organizer
      第10回日本エピジェネティクス研究会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス胎仔海馬由来初代培養ニューロンにおける神経活動依存的DNAメチローム変動の検出2016

    • Author(s)
      亀田朋典、今村拓也、滝沢琢己、木村文香、三浦史仁、伊藤隆司、中島欽一
    • Organizer
      第10回日本エピジェネティクス研究会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] cAMPシグナルによるPC12細胞の最終分化機構には両方向性プロモーター由来のノンコーディングRNA(pancRNA)発現制御が必須である2016

    • Author(s)
      今村拓也、山本直樹、阿形清和、中島欽一:cAMPシグナルによるPC12細胞の最終分化機構には両方向性プロモーター由来のノンコーディングRNA(pancRNA)発現制御が必須である
    • Organizer
      第10回日本エピジェネティクス研究会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪)
    • Year and Date
      2016-05-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 雌性発生胚を活用したPost-Bisulfite Adapter-Tagging法によるマウスDNAメチローム解析2015

    • Author(s)
      今村拓也、浜崎伸彦、上坂将弘、阿形清和、中島欽一
    • Organizer
      第108回日本繁殖生物学会大会
    • Place of Presentation
      宮崎大学(宮崎市)
    • Year and Date
      2015-09-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Bidirectional promoter-derived antisense noncoding RNA epigenetically regulates irreversible differentiation of PC12 cells.2015

    • Author(s)
      Naoki Yamamoto, Masahiro Uesaka, Kiyokazu Agata, Kinichi Nakashima, Takuya Imamura
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Year and Date
      2015-07-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] Gene activation-associated long noncoding RNAs function in mouse preimplantation development.2015

    • Author(s)
      Nobuhiko Hamazaki, Masahiro Uesaka, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura
    • Organizer
      第48回 日本発生生物学会年会 (2015 JSDB-APDBN meeting)
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2015-06-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Species-specific repertories of promoter-associated non-coding RNAs may contribute to the diversification of gene expression profile.2015

    • Author(s)
      Masahiro Uesaka, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura
    • Organizer
      第48回 日本発生生物学会年会 (2015 JSDB-APDBN meeting)
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2015-06-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Species-specific repertories of promoter-associated non-coding RNAs may contribute to the diversification of gene expression profile.2015

    • Author(s)
      Masahiro Uesaka, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura
    • Organizer
      第9回エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京都千代田区)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] Promoter-associated noncoding RNAs mediate gene-specific DNA demethylation for mouse preimplantation development.2015

    • Author(s)
      Nobuhiko, Hamazaki, Masahiro Uesaka, Kinichi Nakashima, Kiyokazu Agata, Takuya Imamura
    • Organizer
      第9回エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京都千代田区)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] Bidirectional promoter-derived antisense noncoding RNA epigenetically regulates irreversible differentiation of PC12 cells.2015

    • Author(s)
      Naoki Yamamoto, Masahiro Uesaka, Kiyokazu Agata, Kinichi Nakashima, Takuya Imamura
    • Organizer
      第9回エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京都千代田区)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] 雌性発生胚を活用したPost-Bisulfite Adapter-Tagging法によるマウスDNAメチローム解析2015

    • Author(s)
      今村拓也、浜崎伸彦、上坂将弘、阿形清和、中島欽一
    • Organizer
      第9回エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京都千代田区)
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
  • [Book] lncRNAの進化と種特異性:「ノンコーディングRNA -RNA分子の全体像を俯瞰する-」廣瀬哲郎・泊 幸秀(編集)2016

    • Author(s)
      亀田朋典・中島欽一・今村拓也
    • Total Pages
      355
    • Publisher
      化学同人
    • Related Report
      2016 Annual Research Report
  • [Remarks] ほ乳類神経幹細胞が変化するメカニズムを明らかに(九州大学プレスリリース)

    • URL

      https://www.kyushu-u.ac.jp/ja/researches/view/171

    • Related Report
      2017 Annual Research Report
  • [Remarks] 研究代表者HP

    • URL

      http://scb.wp.med.kyushu-u.ac.jp/imamura

    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究代表者HP

    • URL

      http://www.scb.med.kyushu-u.ac.jp/imamura

    • Related Report
      2015 Annual Research Report
  • [Remarks] 研究成果:ノンコーディングRNAによる神経モデル細胞が増えない仕組みの発見

    • URL

      http://www.kyushu-u.ac.jp/ja/researches/view/3

    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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