| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
Nuclear transfer technique manipulates the nucleus itself, but attempts to develop technologies to manipulate smaller units, ie chromosomes. We used the suntag-system to label specific chromosomes and succeeded in labeling the Y chromosome in ES cells and showed the possibility of labeling any chromosome. It also showed that chromosomal transfer between live embryos is possible by dispersing the chromosome in embryos. However, we failed to label and manipulate specific chromosomes in embryos at present. In addition, cloned mouse and ntES cell lines can be established from very few cells contained in the urine that can be harvested without harming individuals in the threatened species expected to use chromosomal manipulation.
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