New approach for studying functional importance of baculovirus nonessential genes
| Project/Area Number |
15H04608
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 昌直 北海道大学, 農学研究院, 助教 (20517693)
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| Research Collaborator |
TAKA Hitomi
ISHIBASHI Taiki
HARADA Naoto
SAITO Ryo
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Keywords | 昆虫機能利用 / バキュロウイルス / 有用物質生産 / ロバストな増殖 |
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| Outline of Final Research Achievements |
In Bombyx mori nucleopolyhedrovirus (BmNPV) genome comprised of 143 genes, more than 60 % of them are nonessential genes for efficient multiplication in Bombyx mori cell line BmN. However, a combination of nonessential genes was found to play a critical role in efficient viral multiplication, suggested essentiality of nonessential genes as a collected gene. A study using reverse genetics identified genetic interactions among nonessential gens in such a combination and emphasized importance of a methodology to advance the reverse combinatorial genetics of BmNPVs; thus we established a platform for BmNPV genome assembly from PCR-amplified DNA fragments using Gibson Assembly for further analysis of the essentiality of nonessential genes and for reconstruction of BmNPV vector system based on logical design.
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Report
(4 results)
Research Products
(2 results)
