Endocrine mechanisms regulating the growth and development of imaginal disks.

• Project/Area Number: 15H04610
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Insect science
• Research Institution: Aichi Gakuin University
• Principal Investigator: Mizoguchi Akira 愛知学院大学, 教養部, 教授 (60183109)
• Co-Investigator(Kenkyū-buntansha): 八木 克将 名古屋大学, 理学研究科, 助教 (10372525) ; 塩見 邦博 信州大学, 学術研究院繊維学系, 准教授 (70324241)
• Research Collaborator: Fujinaga Daiki 東京大学, 新領域創成科学研究科, 大学院生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000); Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: 昆虫 / 成虫原基 / エクジステロイド / IGF様ぺプチド / 細胞内シグナル伝達 / 成長 / カイコガ / IGF様ペプチド / シグナル伝達経路 / 変態 / ホルモン / 発生・分化

Outline of Final Research Achievements

A large quantity of an insulin-like growth faster (IGF)-like peptide is secreted after pupation in Bombyx mori. Hypothesizing that the growth and development of adult tissues are regulated by the combined effects of this peptide (BIGFLP) and 20-hydroxyecdysone (20E), we investigated the growth-promoting effects of these hormones on the male genital disks of B. mori cultured in vitro and further analyzed the cell signaling pathways mediating hormone actions. We revealed that 20E induces the elongation of genital disks, that both hormones stimulate protein synthesis in an additive manner, and that BIGFLP and 20E exert their effects through the insulin/IGF signaling pathway and MAPK pathway, respectively. These results show that the growth and development of the genital disk are coordinately regulated by both BIGFLP and 20E. The analyses using a BIGFLP-deficient mutant produced by CRSPR-Cas9 system showed that BIGFLP plays a critical role in the growth control of ovaries.

Research Products

• [Journal Article] Insulin-like growth factor (IGF)-like peptide and 20-hydroxyecdysone regulate the growth and development of the male genital disk through different mechanisms in the silkmoth, Bombyx mori (2017)
  • Author(s): Fujinaga Daiki、Kohmura Yusuke、Okamoto Naoki、Kataoka Hiroshi、Mizoguchi Akira
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 87 Pages: 35-44
  • DOI: 10.1016/j.ibmb.2017.06.003
  • Peer Reviewed
• [Journal Article] Endocrine changes during diapause development in the cabbage army moth Mamestra brassicae (2017)
  • Author(s): Yamada Nobuto、Mizoguchi Akira
  • Journal Title: Physiological Entomology Volume: 42 Issue: 3 Pages: 239-245
  • DOI: 10.1111/phen.12189
  • Peer Reviewed
• [Journal Article] Identification of ecdysteroidogenic enzyme genes and their expression during pupal diapause in the cabbage armyworm, Mamestra brassicae. (2017)
  • Author(s): Ogihara MH, Ikeda H, Yamada N, Hikiba J, Nakaoka T, Fujimoto Y, Suzuki Y, Saito K, Mizoguchi A, Kataoka H
  • Journal Title: Insect Molecular Biology Volume: 26(3) Issue: 3 Pages: 286-297
  • DOI: 10.1111/imb.12291
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Myosuppression is involved in the regulation of pupal diapause in the cabbage army moth Mamestra brassicae.7 (2017)
  • Author(s): Yamada, N., Kataoka, H., Mizoguchi, A.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 41651-41651
  • DOI: 10.1038/srep41651
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Rab proteins in the brain and corpus allatum of Bombyx mori (2016)
  • Author(s): Uno.T., Furutani,M.,Watanabe,C., Sakamoto.K., Uno,Y., Kanamaru,K.,
  • Journal Title: Histochemistry and Cell Biology Volume: 未定 Issue: 1 Pages: 59-69
  • DOI: 10.1007/s00418-016-1422-y
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Endocrine Mechanisms Regulating Post-Diapause Development in the Cabbage Armyworm, Mamestra brassicae. (2016)
  • Author(s): Yamada, N., Okamoto, N., Kataoka, H., Mizoguchi, A.
  • Journal Title: PLos One Volume: 11 Issue: 1 Pages: e0146619-e0146619
  • DOI: 10.1371/journal.pone.0146619
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Isoform-specific expression of the neuropeptide orcokinin in Drosophila melanogaster. (2015)
  • Author(s): Chen, J., Choi, M.S., Mizoguchi, A., Veenstra, J.A., Kang, K., Kim, Y.-J., Kwon, J.Y.
  • Journal Title: Peptides Volume: 68 Pages: 50-57
  • DOI: 10.1016/j.peptides.2015.01.002
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx. (2015)
  • Author(s): Shiomi, K., Takasu, Y., Kunii, M., Tsuchiya, R., Mukaida, M., Kobayashi, M., Sezutsu, H., Ichida, M., Mizoguchi, A.
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 15566-15566
  • DOI: 10.1038/srep15566
  • NAID: 120007101413
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 蛹休眠の調節機構 ―ヨトウガの蛹休眠を中心に－ (2015)
  • Author(s): 溝口明
  • Journal Title: 蚕糸・昆虫バイオテック Volume: 84 Pages: 135-143
  • NAID: 130006162018
• [Presentation] カイコガIGF様ペプチド遺伝子欠損変異体の解析 (2018)
  • Author(s): 藤永大輝、八木克将、塩見邦博、片岡宏誌、溝口明
  • Organizer: 日本蚕糸学会
• [Presentation] ヨトウガ蛹休眠の休眠覚醒後の成虫発生を制御する内分泌機構 (2015)
  • Author(s): 山田信人、溝口明
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟）
  • Year and Date: 2015-09-17
• [Presentation] カイコガ生殖器原基の成長発達のホルモン調節機構 (2015)
  • Author(s): 藤永大輝、溝口明
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟）
  • Year and Date: 2015-09-17
• [Book] Handbook of Hormones: Comparative Endocrinology for Basic and Clinical Research (2015)
  • Author(s): Mizoguchi, A.
  • Publisher: Academic Press