| Project/Area Number |
15H04611
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | Kyushu University |
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Principal Investigator |
Yamamoto Koji 九州大学, 農学研究院, 助教 (00346834)
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| Co-Investigator(Kenkyū-buntansha) |
坪田 拓也 国立研究開発法人農業生物資源研究所, その他部局等, 主任研究員 (00612772)
有竹 浩介 第一薬科大学, 薬学部, 教授 (70390804)
東浦 彰史 大阪大学, たんぱく質研究所, 助教 (90598129)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | プロスタグランジン / プロスタグランジン合成酵素 / カイコ / 酵素 / 昆虫 |
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| Outline of Final Research Achievements |
Knowledge on role and synthesis of prostaglandins (PGs) in insect are limited. PGE synthase (PGES) and PGD synthase (PGDS) were already identified, and three-dimensional structure of PGES was determined.
In this study, we examined physiological role of PG by using genome-editing, TALEN, and X-ray analysis. In knock-out mutant of PGES, PG was not present by using mass-spectrometry. Since we were interested in composition of the active sites in PGDS, we determined crystal structure of PGDS. Based on the PGDS structure and site-directed mutagenesis, we identified the amino acid residues (Tyr8、 Lue14、Trp39、Lys43、Gln50、Val51、Gln63、and Ser64) involved in catalytic function.
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