| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Outline of Final Research Achievements |
Insect cell expression systems are known as excellent systems for secreted protein production with glycosylation modification, although the structure of the sugar chains is different from that of mammalian-derived cells. In the field of drug discovery, there is a need for humanization of protein sugar chains expressed in insect cells. There are two types of sugar chain modification, N-type and O-type, in sugar chain modification. As for the N-type sugar chain, insect cell lines introduced several human-derived glycosyltransferases had been was established, and partially succeeded in humanizing the recombinant glycoprotein expressed by the baculovirus expression system. In addition, we identified genes involved in the first and second reactions of the O-type sugar chain modification of insect cells, for which there was almost no knowledge so far, and clarified their properties.
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