| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Outline of Final Research Achievements |
It is well known that existence of the ice nucleation substances (ice nucleators) such as silver iodide and ice nucleation bacteria promote freezing of supercooled water. Because these substances catalyze freezing of water, ice nucleation activity (INA) increases freezing temperature of the solution. On the other hand, recent studies showed that some polyphenols repressed INA of ice nucleators and depressed freezing temperature of the solution containing ice nucleators. Existence of these polyphenols (anti-INA polyphenols) maintained supercooling state of the solution. For study on the anti-INA process by anti-INA polyphenols, His-inaA was highly purified from the soluble fraction prepared from the crude extracts of Escherichia coli cells overexpressing His-inaA and soluble form of His-inaA was characterized. INA was detected in the soluble form of His-inaA and repressed by addition of some anti-INA polyphenols.
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