Analysis of roles of nuclear actin family in the maintenance of genome stability and operation of their functions using peptides
| Project/Area Number |
15H04625
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
太田 邦史 東京大学, 大学院総合文化研究科, 教授 (90211789)
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| Research Collaborator |
TASHIRO satoshi
KIYOTA hiromasa
Gasser Susan M.
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | アクチンファミリー / 細胞核 / クロマチン / 遺伝子機能 / 核内アクチン / ゲノム安定性 / DNA修復 / エピジェネティック制御 |
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| Outline of Final Research Achievements |
By using mutants of the nuclear actin-related protein Arp4, we showed that the nuclear actin family member plays important roles in epigenetic regulation of DNA damage repair. Then, we obtained bicyclic peptides binding to G-actin, F-actin, Arp5, and Arp8. We successfully introduced these bicyclic peptides into living cells and analyzed functions of these actin family members in living cells.
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| Academic Significance and Societal Importance of the Research Achievements |
クロマチン・細胞核によるゲノム機能制御は「エピジェネティクス」とよばれ、発生・分化・老化・疾病などの高次生命現象と密接に関連しており、またエピジェネティクス機構の破綻はガンなどの疾病を引き起こすことから、その解明が急がれている。本研究において、細胞核内のアクチンファミリーのエピジェネティック制御への関与の一端が明らかとなり、またその操作の可能性が示されたことは、エピジェネティック制御機構の解明や応用に貢献する。最近では、農畜水産物の生産や育種や、食品機能性評価にエピジェネティクスを応用することも進んでおり、将来的な農学分野への本研究成果の応用も期待される。
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Report
(4 results)
Research Products
(77 results)
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[Journal Article] Distinct roles of ATM and ATR in the regulation of ARP8 phosphorylation to prevent chromosome translocations2018
Author(s)
Sun, J., Shi, L., Kinomura, A., Fukuto, A., Horikoshi, Y., Oma, Y., Harata, M., Ikura, M., Ikura, T., Kanaar, R., Tashiro, S
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Journal Title
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Actin Family Proteins in the Human INO80 Chromatin Remodeling Complex Exhibit Functional Roles in the Induction of Heme Oxygenase-1 with Hemin.2017
Author(s)
Takahashi, Y., Murakami, H., Akiyama, Y., Katoh, Y., Oma, Y., Nishijima, H., Shibahara, K.I., Igarashi, K., Harata, M.
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Journal Title
Front. Genet.
Volume: 印刷中
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] Analyses of molecular evolution of the histone variant H2A.Z2017
Author(s)
S. Kitagawa, M. Kusakabe, H. Oku, D. Takahashi, T. Narimiya, Y. Nakabayashi, M. Seki, M. Harata
Organizer
Tohoku Forum for Creativity Thematic Program 2017 New Horizons in Food Science via Agricultural Immunology, Stage 2 International symposium and youth program, Food Safety and Functional Evaluation
Related Report
Int'l Joint Research
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