| Project/Area Number |
15H04629
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
三上 文三 京都大学, 農学研究科, 教授 (40135611)
丸山 如江 摂南大学, 理工学部, 助教 (90397563)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
MURATA Kousaku 摂南大学, 理工学部, 教授 (90142299)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
|
|---|
| Keywords | 病原性細菌 / 細胞外マトリクス / グリコサミノグリカン / フォスフォトランスフェラーゼ系 / ABCトランスポーター / 分解・代謝酵素 / 遺伝子クラスター / X線結晶構造解析 / 連鎖球菌 / 連鎖桿菌 / 細胞外マトリックス / 細菌 / 分解系 / 輸送系 / 宿主細胞外マトリクス / 代謝酵素 / 動物細胞外マトリクス / 酵素 / 輸送体 / 分解酵素 |
|---|
| Outline of Final Research Achievements |
For colonization and invasion to animal cells, some pathogenic bacteria such as Streptococcus target glycosaminoglycans present in host extracellular matrices. In this study, streptococcal phosphotransferase system involved in the import of a glycosaminoglycan hyaluronan as well as the bacterial degradation/metabolism mechanisms of the fragmented hyaluronan were clarified by molecular and structural biology. A novel ABC transporter was also identified in Streptobacillus to be an importer of fragmented glycosaminoglycans. Based on results obtained here, inhibitors of these bacterial molecular machineries are expected to become promising agents for the bacterial infectious diseases.
|
