Creation of regeneration accelerating molecules using target protein oriented natural products isolation (TPO-NAPI)

• Project/Area Number: 15H04650
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Chiba University
• Principal Investigator: Arai Midori 千葉大学, 大学院薬学研究院, 准教授 (40373261)
• Co-Investigator(Renkei-kenkyūsha): ISHIBASHI Masami 千葉大学, 大学院薬学研究院, 教授 (90212927) ; KOUDA Masao 筑波大学, 医学医療系サイバニクス研究センター, 准教授 (50361449) ; TAMURA Yutaka 千葉大学, 大学院医学研究院, 准教授 (50263174) ; ARAI Takayoshi 千葉大学, 大学院理学研究院, 教授 (80272483)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 幹細胞 / 天然物 / タンパク質ビーズ / ヘッジホッグ / ノッチ / Hes1 / 神経分化 / タンパク質 / 再生 / ケミカルバイオロジー

Outline of Final Research Achievements

Search for stem cells modulators has been done using screening system of natural resources extracts library. Bioactive natural products which bind on Hes1 (bHLH factor), GLI1 (Hedgehog signaling transcriptional factor) and NICD (Notch signaling transcriptional factor ) were efficiently found by using “target protein oriented natural products isolation (TPO-NAPI)”. In addition, bioactivity fractionation using cell-based reporter assay gave Notch signal inhibitors such as uscharin.

Research Products

• [Int'l Joint Research] khulna University/University of Dhaka(バングラデシュ)
• [Int'l Joint Research] Khon Kaen University(タイ)
• [Int'l Joint Research] Khulna University/University of Dhaka(バングラデシュ)
• [Int'l Joint Research] Khon Kaen University(タイ)
• [Journal Article] The Notch inhibitor cowanin accelerates nicastrin degradation (2018)
  • Author(s): Arai, M. A.*; Akamine, R.; Tsuchiya, A.; Yoneyama, T.; Koyano, T.; Kowithayakorn, T.; Ishibashi, M.*
  • Journal Title: Sci. Rep. Volume: 8 Issue: 1 Pages: 5376-5376
  • DOI: 10.1038/s41598-018-23698-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Synthesis of lignan glycosides with antioxidant activity (2018)
  • Author(s): Sampei, M.; Arai, M. A.*; Ishibashi, M.*
  • Journal Title: J. Nat. Med. Volume: in press Issue: 3 Pages: 651-654
  • DOI: 10.1007/s11418-018-1198-6
  • Peer Reviewed
• [Journal Article] New flavanol dimers from the bark of Celtis tetranda and their TRAIL resistance-overcoming activity (2018)
  • Author(s): Seephonkai, P.; Ishikawa, R.; Arai, M. A.; Kowithayakorn, T.; Koyano, T.; Ishibashi, M.
  • Journal Title: Nat. Prod. Commun. Volume: in press
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dehydropropylpantothenamide isolated by a co-culture of Nocardia tenerifensis IFM 10554T in the presence of animal cells (2018)
  • Author(s): Hara, Y.; Arai, M. A.; Sakai, K.; Ishikawa, N.; Gonoi, T.; Yaguchi, T.; Ishibashi, M.
  • Journal Title: J. Nat. Med. Volume: 72 Issue: 1 Pages: 280-289
  • DOI: 10.1007/s11418-017-1161-y
  • NAID: 40021456166
  • Peer Reviewed
• [Journal Article] Isolation of resistomycin from a terrestrial actinomycete with TRAIL resistance-overcoming activity (2018)
  • Author(s): Liu, X.; Arai, M. A.; Toume, K.; Ishibashi, M.
  • Journal Title: Nat. Prod. Commun. Volume: 13 Pages: 65-66
  • Peer Reviewed
• [Journal Article] Notch Inhibitors from Calotropis gigantea That Induce Neuronal Differentiation of Neural Stem Cells (2017)
  • Author(s): Yoneyama Tatsuro、Arai Midori A.、Akamine Ryuta、Koryudzu Kazune、Tsuchiya Anna、Sadhu Samir K.、Ahmed Firoj、Itoh Motoyuki、Okamoto Ryuichi、Ishibashi Masami
  • Journal Title: Journal of Natural Products Volume: 80 Issue: 9 Pages: 2453-2461
  • DOI: 10.1021/acs.jnatprod.7b00282
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Boesenberols I?K, new isopimarane diterpenes from Boesenbergia pandurata with TRAIL-resistance overcoming activity (2017)
  • Author(s): Karmakar Utpal K.、Arai Midori A.、Koyano Takashi、Kowithayakorn Thaworn、Ishibashi Masami
  • Journal Title: Tetrahedron Letters Volume: 58 Issue: 40 Pages: 3838-3841
  • DOI: 10.1016/j.tetlet.2017.08.055
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Total Synthesis of Agalloside, Isolated from Aquilaria agallocha, by the 5-O-Glycosylation of Flavan (2017)
  • Author(s): Arai, M. A.*; Yamaguchi, Y.; Ishibashi, M.*
  • Journal Title: Org. Biomol. Chem. Volume: 15 Issue: 23 Pages: 5025-5032
  • DOI: 10.1039/c7ob01004d
  • Peer Reviewed
• [Journal Article] Efficient Synthesis of Heterocyclic Flavonoids with Hedgehog Signal Inhibitory Activity (2017)
  • Author(s): Arai, M. A.*; Utsumi, T.; Yanase, N.; Fujimatsu, T.; Ishibashi, M.*
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 65 Issue: 8 Pages: 784-795
  • DOI: 10.1248/cpb.c17-00305
  • NAID: 130005875866
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed
• [Journal Article] Hes1 binding compounds isolated by Target Protein Oriented Natural Products Isolation (TPO-NAPI) (2017)
  • Author(s): Arai, M. A.*; Tanaka, M.; Tanouchi, K.; Ishikawa, N.; Ahmed, F.; Sadhu, S. K.; Ishibashi, M.*
  • Journal Title: J. Nat. Prod. Volume: 80 Issue: 2 Pages: 538-543
  • DOI: 10.1021/acs.jnatprod.6b01072
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Isolation and evaluation of hedgehog inhibitors from Christmas grass (Themeda arguens) (2017)
  • Author(s): Yoneyama, T.; Arai, M. A.*; Koyano, T.; Kowithayakorn, T.; Ishibashi, M.
  • Journal Title: Heterocycles Volume: 印刷中 Issue: 1 Pages: 210-210
  • DOI: 10.3987/com-16-s(s)4
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Valosin-containing Protein is a Target of 5'-l Fuligocandin B and Enhances TRAIL Resistance in Cancer Cells (2016)
  • Author(s): Arai, M. A.*; Taguchi, S.; Komatsuzaki, K.; Uchiyama, K.; Masuda, A.; Sampei, M.; Satoh, M.; Kado, S.; Ishibashi, M.*
  • Journal Title: ChemistryOPEN Volume: 5 Issue: 6 Pages: 574-579
  • DOI: 10.1002/open.201600081
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Cerasoidine, a bis-aporphine alkaloid isolated from Polyalthia cerasoides during screening for Wnt signal inhibitors (2016)
  • Author(s): Shono, T.; Ishikawa, N.; Toume, K.; Arai, M. A.; Masu, H.; Koyano, T.; Kowithayakorn, T.; Ishibashi, M.
  • Journal Title: J. Nat. Prod. Volume: 79 Issue: 8 Pages: 2083-2088
  • DOI: 10.1021/acs.jnatprod.6b00409
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Stereochemical assignment and biological evaluation of BE-14106 unveils the importance of one acetate unit for the antifungal activity of polyene macrolactams (2016)
  • Author(s): Fujita, K.; Sugiyama, R.; Nishimura, S.; Ishikawa, N.; Arai, M. A.; Ishibashi, M.; Kakeya, H.
  • Journal Title: J. Nat. Prod. Volume: 79 Issue: 7 Pages: 1877-1880
  • DOI: 10.1021/acs.jnatprod.6b00250
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Boesenberols, pimarane diterpenes with TRAIL resistance-overcoming activity from Boesenbergia pandurata (2016)
  • Author(s): Karmakar, U. K.; Ishikawa, N.; Arai, M. A.; Ahmed, F.; Koyano, T.; Kowithayakorn, T.; Ishibashi, M.
  • Journal Title: J. Nat. Prod. Volume: 79 Issue: 8 Pages: 2075-2082
  • DOI: 10.1021/acs.jnatprod.6b00424
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Bioactive Secondary Metabolites with Unique Aromatic and Heterocyclic Structures Obtained from Terrestrial Actinomycetes Species (2016)
  • Author(s): Abdelfatta, M. S., Arai, M. A., and Ishibashi, M.
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 64 Issue: 7 Pages: 668-675
  • DOI: 10.1248/cpb.c16-00038
  • NAID: 130005160043
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Synthesis of rocaglamide derivatives and evaluation of their Wnt signal inhibitory activities (2016)
  • Author(s): Arai, M. A.*; Kofuji, Y.; Tanaka, Y.; Yanase, N.; Yamaku, K.; Fuentes, R. G.; Karmakar, U. K.; Ishibashi, M.*
  • Journal Title: Org. Biomol. Chem. Volume: 14 Issue: 11 Pages: 3061-3068
  • DOI: 10.1039/c5ob02537k
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Hes1 dimer inhibitor isolated by target protein oriented natural products isolation (TPO-NAPI) of differentiation activators of neural stem cells (2016)
  • Author(s): Arai, M. A.*; Ishikawa, N.; Tanaka, M.; Uemura, K.; Sugimitsu, N.; Koyano, T.; Kowithayakorn, T.; Ishibashi, M.*
  • Journal Title: Chem. Sci. Volume: 7 Issue: 2 Pages: 1514-1520
  • DOI: 10.1039/c5sc03540f
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Coronaridine, an iboga type alkaloid from Tabernaemontana divaricata, inhibits Wnt signaling pathway by decreasing β-catenin mRNA expression (2015)
  • Author(s): Ohishi, K.; Toume, K.; Arai, M. A.; Sadhu, S. K.; Ahmed, F.; Ishibashi, M.
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 25 Issue: 18 Pages: 3937-3940
  • DOI: 10.1016/j.bmcl.2015.07.036
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Hedgehog inhibitors from Withania somnifera (2015)
  • Author(s): Yoneyama, T.; Arai, M. A.*; Sadhu, S. K.; Ahmed, F.; Ishibashi, M.*
  • Journal Title: Bioorg. Med. Chem. Lett. Volume: 25 Issue: 17 Pages: 3541-3544
  • DOI: 10.1016/j.bmcl.2015.06.081
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Hedgehog inhibitors from Artocarpus communis and Hyptis suaveolens (2015)
  • Author(s): Arai, M. A.*; Uchida, K.; Sadhu, S. K.; Ahmed, F.; Koyano, T.; Kowithayakorn, T.; Ishibashi, M.*
  • Journal Title: Bioorg. Med. Chem. Volume: 23 Issue: 15 Pages: 4150-4154
  • DOI: 10.1016/j.bmc.2015.06.058
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Hedgehog/GLI- mediated transcriptional activity inhibitors from Crinum asiaticum (2015)
  • Author(s): Arai, M. A.*; Akamine, R.; Sadhu, S. K.; Ahmed, F.; Ishibashi, M.*
  • Journal Title: J. Nat. Med. Volume: 69 Issue: 4 Pages: 538-542
  • DOI: 10.1007/s11418-015-0922-8
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Boehmenan, a lignan, isolated from Hibiscus ficulneus showed Wnt signal inhibitory activity (2015)
  • Author(s): Takumi Shono
  • Journal Title: Bioorganic and Medicinal Chemistry Letters Volume: 25 Issue: 14 Pages: 2735-2738
  • DOI: 10.1016/j.bmcl.2015.05.037
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phenolic compounds from the bark of Oroxylum indicum identified as Ngn2 promoter activators (2015)
  • Author(s): Fuentes, R. G.; Arai, M. A.*; Sadhu, S. K.; Ahmed, F.; Ishibashi, M.*
  • Journal Title: J. Nat. Med. Volume: 78 Pages: 864-872
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 9-Hydorxycanthin-6-one, a β-carboline alkaloid from Eurycoma longifolia, is the first Wnt signal inhibitor through activation of GSK3β without depending on CK1α (2015)
  • Author(s): Ohishi, K.; Toume, K.; Arai, M. A.; Koyano, T.; Kowithayakorn, T.; Mizoguchi, T.; Itoh, M.; Ishibashi, M.
  • Journal Title: J. Nat. Prod. Volume: 11 Issue: 5 Pages: 1139-1146
  • DOI: 10.1021/acs.jnatprod.5b00153
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Limonoids with Wnt signal inhibitory activity isolated from the fruits of Azadirachta excelsa (2015)
  • Author(s): Fuentes, R. G.; Toume, K.; Arai, M. A.; Sadhu, S. K.; Ahmed, F.; Ishibashi, M.
  • Journal Title: Phytochem. Lett. Volume: 11 Issue: 4 Pages: 280-285
  • DOI: 10.1007/s11418-015-0919-3
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Inubosin A, B, and C are new acridine alkaloids isolated from Streptomyces sp. IFM 11440 that show Ngn2 promoter activity (2015)
  • Author(s): Midori A. Arai
  • Journal Title: Journal of Natural Products Volume: 78 Issue: 2 Pages: 311-314
  • DOI: 10.1021/np5006218
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Naturally occurring FANCF-Hes1 complex inhibitors from Wrightia religiosa (2015)
  • Author(s): Midori A. Arai
  • Journal Title: Med. Chem. Commun. Volume: 6 Issue: 3 Pages: 455-460
  • DOI: 10.1039/c4md00495g
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Natural compounds with Wnt signal modulating activity (2015)
  • Author(s): R. G. Fuentes, M. A. Arai, and M. Ishibashi
  • Journal Title: Nat. Prod. Rep. Volume: 32 Issue: 12 Pages: 1622-1628
  • DOI: 10.1039/c5np00074b
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] TRAIL耐性克服作用を有する天然物furigocandin B誘導体の合成 (2017)
  • Author(s): 荒井緑，増田彩花，石橋正己
  • Organizer: 日本薬学会第137会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-25
• [Presentation] Notchシグナル阻害作用を有する光学活性スピロ化合物の合成研究究 (2017)
  • Author(s): 荒井緑，岡野美優，荒井孝義，石橋正己
  • Organizer: 日本薬学会第137会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-25
• [Presentation] 神経幹細胞分化を促進するHes1二量体形成阻害剤の合成研究 (2017)
  • Author(s): 荒井緑，山口祐未，石川直樹，石橋正己
  • Organizer: 日本薬学会第137会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-25
• [Presentation] リグナン骨格を有する神経幹細胞分化促進剤の創製研究 (2017)
  • Author(s): 三瓶真菜，荒井 緑，石橋正己
  • Organizer: 日本薬学会第137会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-25
• [Presentation] Eugenia operculata等からのBMI1プロモーター阻害作用をもつ天然物の探索 (2017)
  • Author(s): 金田結，荒井緑，石川直樹，小谷野喬，T. Kowithayakorn, 千葉哲博，岩間厚志，石橋正己
  • Organizer: 日本薬学会第137会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-25
• [Presentation] Celtis tetrandraとAmonum villosumからのTRAIL耐性克服作用をもつ天然物の探索 (2017)
  • Author(s): P. Seephonkai, 荒井緑，石川直樹，小谷野喬，T. Kowithayakorn，石橋正己
  • Organizer: 日本薬学会第137会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-25
• [Presentation] ヘテロ環を有するrocaglamide誘導体の合成と活性評価 (2017)
  • Author(s): 荒井緑，田中優貴，小藤悠貴，石橋正己
  • Organizer: 日本薬学会第137会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-25
• [Presentation] Approach to neural stem cells and cancer cells based on natural products (2017)
  • Author(s): M. A. Arai
  • Organizer: The 4th Joint Symposium on Pharmaceutical Sciences between Graduate School of Pharmaceutical Sciences, Chiba University and College of Pharmacy, Seoul National University
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2017-02-10
  • Int'l Joint Research / Invited
• [Presentation] Target identification and mode of action of 5’-I fuligocandin B with TRAIL-resistance overcoming activity (2017)
  • Author(s): M. A. Arai
  • Organizer: Asian Chemical Biology Initiative 2017
  • Place of Presentation: Ho Chi Minh, Vietnam
  • Year and Date: 2017-01-20
  • Int'l Joint Research / Invited
• [Presentation] 天然物を基盤とした生物活性分子の探索と創製 (2017)
  • Author(s): 荒井緑
  • Organizer: 東京工業大学 化学生命科学研究所講演
  • Invited
• [Presentation] Target identification and mode of action of 5’-I fuligocandin B with TRAIL-resistance overcoming activity (2017)
  • Author(s): M. A. Arai
  • Organizer: WCCU-SMA Joint Symposium 2018
  • Int'l Joint Research / Invited
• [Presentation] Synthesis of rocaglamide derivatives with Wnt signal inhibition (2017)
  • Author(s): Y. Makita, M. A. Arai, Y. Tanaka, M. Ishibashi
  • Organizer: The Second Symposium of Chiral Molecular Science and Technology in Chiba University
  • Int'l Joint Research
• [Presentation] Notch シグナル阻害作用を持つ放線菌代謝産物の成分探索 (2017)
  • Author(s): 合田芙二子，荒井緑，林奈留美，石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] Notchシグナル阻害作用を有する天然物の探索 (2017)
  • Author(s): 土屋杏奈，荒井緑，小谷野喬，T. Kowithayakorn，S. K. Sadhu, F. Ahmed, 石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] Ricinus communisとClinogyne dichotomaからのWntシグナル阻害作用をもつ天然物の探索 (2017)
  • Author(s): 佐藤知幸，荒井緑，S. K. Sadhu, F. Ahmed, 石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] Amonum villosum等からのTRAIL耐性克服作用をもつ天然物の探索 (2017)
  • Author(s): 石川龍翔，Prapairat Seephonkai，荒井緑，S. K. Sadhu, F. Ahmed, 小谷野喬，T. Kowithayakorn，石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] 細胞毒性をもつMurraya paniculata抽出物からのクマリン型化合物の探索 (2017)
  • Author(s): 戸次雄一，荒井緑，小谷野喬，T. Kowithayakorn，石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] Hes1二量体形成阻害活性を有する神経幹細胞分化活性化剤の探索 (2017)
  • Author(s): 盛田かおり，荒井緑，S. K. Sadhu, F. Ahmed, 石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] Nocardia属放線菌からの新規二次代謝産物の探索 (2017)
  • Author(s): 小林菜摘，原康雅，荒井緑，五ノ井透，矢口貴志，石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] 放線菌二次代謝産物からのBMI1プロモーター阻害剤の探索 (2017)
  • Author(s): 横山夕将，荒井緑，石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] 動物細胞との共培養法による放線菌Nocardia属由来二次代謝産物の探索 (2017)
  • Author(s): 原康雅，荒井緑，五ノ井透，矢口貴志，石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] NICDビーズを用いたNotchシグナル阻害剤の探索 (2017)
  • Author(s): 林奈留美，荒井緑，菅波晃子，田村裕，石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] リグナン骨格を有する神経幹細胞分化促進剤の創成研究 (2017)
  • Author(s): 三瓶真菜，荒井緑，菅波晃子，田村裕，石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] Wntシグナル阻害作用を有するロカグラミド誘導体の創成 (2017)
  • Author(s): 蒔田吉功，荒井緑，田中優貴，石橋正己
  • Organizer: 日本薬学会第138年会
• [Presentation] Target Protein Oriented Natural Products Isolation (TPO-NAPI) (2017)
  • Author(s): M. A. Arai
  • Organizer: The Second A3 Round Table Meeting
  • Int'l Joint Research / Invited
• [Presentation] ,Approach to neural stem cells and cancer cells based on natural products (2017)
  • Author(s): M. A. Arai
  • Organizer: The 7th Sino-Japanese Symposium on Organic Chemistry for Young Scientists
  • Int'l Joint Research / Invited
• [Presentation] 天然物を基盤とした生物活性分子の探索と創製 (2017)
  • Author(s): 荒井緑
  • Organizer: 第一回函館セミナー
  • Invited
• [Presentation] GLI1ビーズを用いたヘッジホッグシグナル阻害剤の探索 (2017)
  • Author(s): 越智富美江，荒井緑，S. K. Sadhu，F. Ahmed，石橋正己
  • Organizer: 第7回食品薬学シンポジウム
• [Presentation] Target Protein Oriented Natural Product Isolation (2017)
  • Author(s): M. A. Arai
  • Organizer: Asian Chemical Biology Initiative 2017, Ulaanbaatar Meeting
• [Presentation] Notch シグナル阻害作用を有する光学活性スピロ化合物の創成研究 (2017)
  • Author(s): 岡野美優，荒井緑，荒井孝義，石橋正己
  • Organizer: 第47回複素環化学討論会
• [Presentation] TRAIL 耐性克服作用を有する天然物fuligocandin B 誘導体の合成 (2017)
  • Author(s): 増田彩花，荒井緑，石橋正己
  • Organizer: 第47回複素環化学討論会
• [Presentation] Wnt シグナル阻害作用を有するロカグラミド誘導体の創成 (2017)
  • Author(s): 蒔田吉功，荒井緑，田中優貴，石橋正己
  • Organizer: 第47回複素環化学討論会
• [Presentation] リグナン骨格を有する神経幹細胞分化促進剤の創成研究 (2017)
  • Author(s): 三瓶真菜，荒井緑，石橋正己
  • Organizer: 第47回複素環化学討論会
• [Presentation] Wntシグナル阻害作用を有すうるロカグラミド誘導体の創成 (2017)
  • Author(s): 蒔田吉功，荒井緑，石橋正己
  • Organizer: 分子キラリティーセンターミーティング
• [Presentation] NICDビーズを用いたNotchシグナル阻害剤の探索 (2017)
  • Author(s): 林奈留美，荒井緑，石橋正己
  • Organizer: 日本ケミカルバイオロジー学会第12回年会
• [Presentation] 天然由来Notchシグナル阻害剤によるがんへのアプローチ (2017)
  • Author(s): 土屋杏奈，荒井緑，赤嶺隆太，岡本隆一，小谷野喬，Thaworn Kowithayakorn，Samir K. Sadhu，Firoj Ahmed，石橋正己
  • Organizer: 日本ケミカルバイオロジー学会第12回年会
• [Presentation] NICDビーズを用いたNotchシグナル阻害剤の探索 (2017)
  • Author(s): 林奈留美，荒井緑，石橋正己
  • Organizer: 分子キラリティーセンターミーティング
• [Presentation] Synthesis of Hes1 homodimer inhibitors accelerating the differentiation of neural stem cells (2016)
  • Author(s): M. A. Arai, Y. Yamaguchi, N. Ishikawa, M. Ishibashi
  • Organizer: 2nd Joint Workshop on Chirality in Chiba University and Soft Molecule Activation
  • Place of Presentation: 千葉
  • Year and Date: 2016-12-20
  • Int'l Joint Research
• [Presentation] Synthesis of rocaglamide derivatives and evaluation of their Wnt signal inhibitory activities (2016)
  • Author(s): M. A. Arai, Y. Tanaka, Y. Kofuji, T. Shono, K. Yamaku, M. Ishibashi
  • Organizer: 2nd Joint Workshop on Chirality in Chiba University and Soft Molecule Activation
  • Place of Presentation: 千葉
  • Year and Date: 2016-12-20
  • Int'l Joint Research
• [Presentation] 天然物を基盤とした生物活性分子の探索と創製 (2016)
  • Author(s): 荒井緑
  • Organizer: 横浜市立大学2016年度教室セミナー
  • Place of Presentation: 横浜
  • Year and Date: 2016-12-16
  • Invited
• [Presentation] 天然物を基盤とした生物活性分子の探索と創製 (2016)
  • Author(s): 荒井緑
  • Organizer: 明治薬科大学認知症創薬資源研究開発センター講習会
  • Place of Presentation: 東京
  • Year and Date: 2016-12-06
  • Invited
• [Presentation] Search for BMI1 promoter inhibitors from Beaumontia murtonii and analysis of their mechanism (2016)
  • Author(s): Y. Kaneta, N. Ishikawa, M. A. Arai, K. Toume, T. Koyano, T. Kowithayakorn, T. Chiba, A. Iwama, M. Ishibashi
  • Organizer: Institute for Global Prominent Research Kickoff Symposium
  • Place of Presentation: 千葉
  • Year and Date: 2016-11-14
  • Int'l Joint Research
• [Presentation] Target identification and mode of action of 5’-I fuligocandin B with TRAIL-resistance overcoming activity (2016)
  • Author(s): M. A. Arai, S. Taguchi, K. Komatsuzaki, K. Uchiyama, A. Masuda, M. Satoh, S. Kado, M. Ishibashi
  • Organizer: International symposium on catalysis and fine chemicals 2016
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2016-11-10
  • Int'l Joint Research
• [Presentation] Wntシグナル伝達経路を阻害するロカグラミド誘導体の合成研究 (2016)
  • Author(s): 荒井緑，田中優貴，小藤悠貴，山来一紀，石橋正己
  • Organizer: 第42回反応と合成の進歩シンポジウム
  • Place of Presentation: 静岡
  • Year and Date: 2016-11-07
• [Presentation] Beaumontia murtoniiなどからのBMI1プロモーター阻害剤の探索及び活性評価 (2016)
  • Author(s): 金田結，石川直樹，荒井緑，當銘一文，小谷野喬，T. Kowithayakorn, 千葉哲博，岩間厚志，石橋正己
  • Organizer: 第21回天然薬物の開発と応用シンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2016-10-28
• [Presentation] 神経幹細胞分化を促進するHes1二量体形成阻害剤の合成 (2016)
  • Author(s): 荒井緑，山口祐未，三瓶真菜，石川直樹，石橋正己
  • Organizer: 第21回天然薬物の開発と応用シンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2016-10-28
• [Presentation] Wntシグナル阻害活性を有するrocaglamide誘導体の合成研究 (2016)
  • Author(s): 荒井緑，田中優貴，小藤悠貴，山来一紀，庄野巧，石橋正己
  • Organizer: 第21回天然薬物の開発と応用シンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2016-10-28
• [Presentation] GLI1ビーズを用いたヘッジホッグシグナル阻害剤の探索 (2016)
  • Author(s): 荒井緑，越智富美江，S. K. Sadhu，F. Ahmed，石橋正己
  • Organizer: 第21回天然薬物の開発と応用シンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2016-10-28
• [Presentation] 神経再生とがんの制御をめざした天然物基盤分子の創成研究 (2016)
  • Author(s): 荒井緑
  • Organizer: 第21回天然薬物の開発と応用シンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2016-10-27
  • Invited
• [Presentation] 標的タンパク質指向型天然物単離による生物活性天然物の探索 (2016)
  • Author(s): 荒井緑，越智富美江，田中光葉，田内伽奈，石川直樹，小谷野喬，T. Kowithayakorn, S. K. Sadhu, F. Ahmed, 石橋正己
  • Organizer: 第46回複素環化学討論会
  • Place of Presentation: 金沢
  • Year and Date: 2016-09-26
• [Presentation] GLI1ビーズを用いたヘッジホッグシグナル阻害剤の探索 (2016)
  • Author(s): 荒井緑，越智富美江，S. K. Sadhu，F. Ahmed，石橋正己
  • Organizer: 日本生薬学会第63年会
  • Place of Presentation: 富山
  • Year and Date: 2016-09-25
• [Presentation] Pimarane diterpenes with TRAIL-resistance overcoming activity from Boesenbergia pandurata (2016)
  • Author(s): U. K. Karmakar，石川直樹，荒井緑，小谷野喬，T. Kowithayakorn，石橋正己
  • Organizer: 日本生薬学会第63年会
  • Place of Presentation: 富山
  • Year and Date: 2016-09-24
• [Presentation] Polyalthia cerasoidesからのWntシグナルを阻害する新規アルカロイド (2016)
  • Author(s): 庄野巧，石川直樹，當銘一文，荒井緑，桝飛雄馬，小谷野喬，T. Kowithayakorn，石橋正己
  • Organizer: 日本生薬学会第63年会
  • Place of Presentation: 富山
  • Year and Date: 2016-09-24
• [Presentation] TRAIL耐性克服作用を有する5’-I Fuligocandin Bの標的タンパク質同定と作用機序解析 (2016)
  • Author(s): 荒井緑，田口翔大，小松﨑一裕，内山健人，増田彩花，佐藤守，荷堂清香，石橋正己
  • Organizer: 第58回天然有機化合物討論会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-15
• [Presentation] 神経幹細胞分化を促進するHes1二量体形成阻害剤の合成 (2016)
  • Author(s): 荒井緑，山口祐未，三瓶真菜，石川直樹，石橋正己
  • Organizer: 分子キラリティー研究センターミーティング
  • Place of Presentation: 千葉
  • Year and Date: 2016-09-08
• [Presentation] Screening studies for natural products on disease and development pathways (2016)
  • Author(s): M. Ishibashi and M. A. Arai
  • Organizer: International Symposium on Natural Products for the Future 2016 (ISNPF2016)
  • Place of Presentation: 徳島
  • Year and Date: 2016-09-03
  • Int'l Joint Research
• [Presentation] 天然物を基盤とした生物活性分子の探索と創製 (2016)
  • Author(s): 荒井緑
  • Organizer: JSPS第189委員会 日本におけるケミカルバイオロジーの新展開
  • Place of Presentation: 東京
  • Year and Date: 2016-06-10
  • Invited
• [Presentation] Search for neural stem cell activators based on natural products (2016)
  • Author(s): M. A. Arai, T. Yoneyama, R. Akamine, R. Okamoto, T. Koyano, T. Kowithayakorn, S. K. Sadhu, F. Ahmed, M. Ishibashi
  • Organizer: Molecular Chirality Asia 2016
  • Place of Presentation: 大阪
  • Year and Date: 2016-04-22
  • Int'l Joint Research
• [Presentation] Polyalthia cerasoidesからのWntシグナルを阻害する天然物の探索 (2016)
  • Author(s): 庄野巧，石川直樹，當銘一文，荒井緑，小谷野喬，T. Kowithayakorn，石橋正己
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜 （横浜）
  • Year and Date: 2016-03-29
• [Presentation] 放線菌からのHes1ビーズを用いた神経幹細胞分化を促進する天然物の探索 (2016)
  • Author(s): 荒井緑，田中光葉，石川直樹，石橋正己
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜 （横浜）
  • Year and Date: 2016-03-29
• [Presentation] Garcinia speciosa等からのNotchシグナル阻害作用を有する天然物の探索 (2016)
  • Author(s): 荒井緑，赤嶺隆太，岡本隆一，小谷野喬，T. Kowithayakorn，石橋正己
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜 （横浜）
  • Year and Date: 2016-03-29
• [Presentation] 神経幹細胞分化を促進するHes1二量体形成阻害剤の合成研究 (2016)
  • Author(s): 荒井緑，山口祐未，三瓶真菜，石川直樹，石橋正己
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜 （横浜）
  • Year and Date: 2016-03-29
• [Presentation] ヘテロ環を有するrocaglamide誘導体の合成と活性評価 (2016)
  • Author(s): 荒井緑，田中優貴，小藤悠貴，石橋正己
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜 （横浜）
  • Year and Date: 2016-03-29
• [Presentation] Calotropis giganteaからのNotchシグナル伝達経路阻害を標的とした神経幹細胞分化促進剤の探索 (2016)
  • Author(s): 米山達朗，荒井緑，岡本隆一，Samir K. Sadhu，Firoj Ahmed，石橋正己
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: パシフィコ横浜 （横浜）
  • Year and Date: 2016-03-29
• [Presentation] Isolation of Notch inhibitors from Calotropis gigantea (2016)
  • Author(s): T. Yoneyama, M. A. Arai，R. Okamoto, S. K. Sadh，F. Ahmed，M. Ishibashi
  • Organizer: Joint Workshop on Chirality in Chiba University (WCCU) and on Soft-Molecule Activation
  • Place of Presentation: 千葉大学 (千葉）
  • Year and Date: 2016-03-17
  • Int'l Joint Research
• [Presentation] Synthesis of rocaglamide derivatives and evaluation of their Wnt signal inhibitory activities (2016)
  • Author(s): M. A. Arai, Yuuki Tanaka, Yuuki Kofuji, Natsuki Yanase, Kazuki Yamaku, Rolly G. Fuentes, Masami Ishibashi
  • Organizer: The First Symposium of Chiral Molecular Science and Technology in Chiba University
  • Place of Presentation: 千葉大学 (千葉）
  • Year and Date: 2016-02-29
  • Int'l Joint Research
• [Presentation] Search for neural stem cell activators based on natural products (2016)
  • Author(s): M. A. Arai
  • Organizer: Asian Chemical Biology Initiative 2016 Jakarta Meeting
  • Place of Presentation: Jakarta (Indonesia)
  • Year and Date: 2016-01-30
  • Int'l Joint Research / Invited
• [Presentation] Search for Notch signaling inhibitors from natural products (2015)
  • Author(s): M. A. Arai, R. Akamine, R. Okamoto, T. Koyano, T. Kowithayakorn, S. K. Sadhu, F. Ahmed, M. Ishibashi
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu (U.S.A.)
  • Year and Date: 2015-12-17
  • Int'l Joint Research
• [Presentation] Isolation of Notch inhibitors from Calotropis gigantea (2015)
  • Author(s): T. Yoneyama, M. A. Arai, R. Okamoto, S. K. Sadhu, F. Ahmed, M. Ishibashi
  • Organizer: Pacifichem 2015
  • Place of Presentation: Honolulu (U.S.A.)
  • Year and Date: 2015-12-17
  • Int'l Joint Research
• [Presentation] 放線菌ライブラリーの構築に基づくWntシグナル阻害作用をもつ天然物の探索 (2015)
  • Author(s): 山来一紀，石川直樹，當銘一文，荒井緑，石橋正己
  • Organizer: 第33回メディシナルケミストリーシンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2015-11-25
• [Presentation] Hibiscus ficulneusおよびPolyalthia cerasoidesからのWntシグナル阻害作用をもつ天然物の探索 (2015)
  • Author(s): 庄野巧，石川直樹，當銘一文，荒井緑，S. K. Sadhu，F. Ahmed，小谷野喬，T. Kowithayakorn，石橋正己
  • Organizer: 第6回食品薬学シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2015-10-31
• [Presentation] 神経幹細胞分化を促進するHes1二量体形成阻害剤の合成研究 (2015)
  • Author(s): 荒井緑，山口祐未，石川直樹，石橋正己
  • Organizer: 第41回反応と合成の進歩シンポジウム
  • Place of Presentation: 大阪
  • Year and Date: 2015-10-27
• [Presentation] Calotropis gigantea からのノッチシグナル伝達経路阻害剤の探索 (2015)
  • Author(s): 米山達朗，荒井緑，岡本隆一，S. K. Sadhu，F. Ahmed，石橋正己
  • Organizer: 日本生薬学会第61年会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-09-11
• [Presentation] Notch-Hes経路阻害を指標とする神経幹細胞分化活性化剤の探索 (2015)
  • Author(s): 荒井緑，赤嶺隆太，米山達朗，岡本隆一，小谷野喬，T. Kowithayakornd，石橋正己
  • Organizer: 第57回天然有機化合物討論会
  • Place of Presentation: 横浜
  • Year and Date: 2015-09-09
• [Presentation] Scoparia dulcisから単離したウィントシグナル阻害作用をもつジテルペン (2015)
  • Author(s): Rolly G. Fuentes，當銘一文，荒井緑，Samir K. Sadhu，Firoj Ahmed，石橋正己
  • Organizer: 第59回香料・テルペンおよび精油化学に関する討論会
  • Place of Presentation: 大阪
  • Year and Date: 2015-09-06
• [Presentation] 神経幹細胞分化活性化を目指した天然基盤分子の探索 (2015)
  • Author(s): 荒井緑
  • Organizer: 新学術領域研究「天然物ケミカルバイオロジー～分子標的と活性制御～」地区ミニシンポジウム・仙台「ケミカルバイオロジーにおける天然物」
  • Place of Presentation: 東北大学 (仙台）
  • Year and Date: 2015-06-30
  • Invited
• [Remarks] 千葉大学大学院薬学研究院 活性構造化学研究室
  • URL: http://www.p.chiba-u.jp/lab/kassei/index.html