Studies on the efficacy of toxin neutralization and safety of Shiga toxin-specific secretory IgA type plantibody produced by practical plants
| Project/Area Number |
15H04660
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | University of Shizuoka |
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Principal Investigator |
Imai Yasuyuki 静岡県立大学, 薬学部, 教授 (80160034)
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| Co-Investigator(Kenkyū-buntansha) |
黒羽子 孝太 静岡県立大学, 薬学部, 講師 (90333525)
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| Co-Investigator(Renkei-kenkyūsha) |
Kobayashi Hirokazu 静岡県立大学, 大学院 食品栄養環境科学研究院, 教授 (80170348)
Niwa Yasuo 静岡県立大学, 大学院 食品栄養環境科学研究院, 助教 (00222191)
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| Research Collaborator |
NAKANISHI Katsuhiro
AKIMOTO Yoshihiro
KAWAKAMI Hayato
MATSUBARA Sachie
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | 抗体医薬 / 植物発現系 / 粘膜免疫 / 免疫グロブリンA / 微生物 |
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| Outline of Final Research Achievements |
Transgenic plants that produce secretory immunoglobulin A (SIgA) specific to Shiga toxin 1 (Stx1) were developed using a model plant (Arabidopsis thaliana) and a vegetable (Lactuca sativa). Considering the initial entry of Stx1 can occur through cytotoxicity to colon epithelial cells, we established an assay using a human colon cell line, Caco-2. Plant-made IgA neutralized the toxicity of Stx1 against Caco-2 cells. Production of secretory component (SC), a component of SIgA, was increased in the transgenic plants by the addition of an endoplasmic reticulum retention signal to SC. When plant-made SC was administered to mice, anti-SC antibodies were induced by intravenous administration while no such antibodies were made by oral administration. This result provides evidence as to the safety of oral application of plant-made biologics.
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Report
(4 results)
Research Products
(19 results)
