Elucidation of functions of genes regulating cell polarity in vivo.
| Project/Area Number |
15H04668
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岩野 智彦 山梨大学, 大学院総合研究部, 助教 (10442930)
吉村 信一郎 大阪大学, 医学系研究科, 講師 (60584521)
國井 政孝 大阪大学, 医学系研究科, 助教 (80614768)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 細胞極性 / 極性輸送 / Rab / SNARE / 上皮細胞 / ノックアウトマウス |
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| Outline of Final Research Achievements |
SNAP23 pancreas-specific knockout (KO) mice showed decreased and increased secretion of amylase and insulin, respectively, which was published in JCB. Brain-specific SNAP23 KO mice showed defects in both cerebral and cerebellar cortex, which is being prepared for publication. We showed defects in apical transport in Rab11a KO mice, which was published in Biology Open.
We identified many genes involved in cell polarity. Two of them, Rab1a and BIG1, an activator of Arf, was knocked out in the mice and the results of BIG1 KO mice was published in Plos One. Other genes, such as Rab1, 11b, syntaxin3 were already knocked out and being analyzed. In addition, we identified novel binding proteins for Rab8 and Rab11. We analyzed their roles in vitro and in vivo and the results were published recently in JCB.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] UPR Transducer BBF2H7 Allows Export of Type II Collagen in a Cargo- and Developmental Stage-Specific Manner2017
Author(s)
Tokiro Ishikawa, Takuya Toyama, Yuki Nakamura, Kentaro Tamada, Hitomi Shimizu, Satoshi Ninagawa, Tetsuya Okada, Yasuhiro Kamei, Tomoko Ishikawa-Fujiwara, Takeshi Todo, Eriko Aoyama, Masaharu Takigawa, Akihiro Harada and Kazutoshi Mori
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Journal Title
Journal of cell biology
Volume: 印刷中
Issue: 6
Pages: 1761-1774
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Chloroquine-Inducible Par-4 Secretion Is Essential for Tumor Cell Apoptosis and Inhibition of Metastasis.2017
Author(s)
Burikhanov R, Hebbar N, Noothi SK, Shukla N, Sledziona J, Araujo N, Kudrimoti M, Wang QJ, Watt DS, Welch DR, Maranchie J, Harada A, Rangnekar VM.
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Journal Title
Cell Reports
Volume: 18
Issue: 2
Pages: 508-519
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells.2016
Author(s)
Nakajo A, Yoshimura SI, Togawa H, Kunii M, Iwano T, Izumi A, Noguchi Y, Watanabe A, Goto A, Sato T, Harada A.
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Journal Title
Journal of Cell Biology
Volume: 212
Issue: 3
Pages: 297-306
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Rab8a vesicles regulate Wnt ligand delivery and Paneth cell maturation at the intestinal stem cell niche.2015
Author(s)
Das S, Yu S, Sakamori R, Vedula P, Feng Q, Flores J, Hoffman A, Fu J, Stypulkowski E, Rodriguez A, Dobrowolski R, Harada A, Hsu W, Bonder EM, Verzi MP, Gao N
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Journal Title
Development
Volume: 142(12)
Issue: 12
Pages: 2147-2162
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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