| Project/Area Number |
15H04668
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岩野 智彦 山梨大学, 大学院総合研究部, 助教 (10442930)
吉村 信一郎 大阪大学, 医学系研究科, 講師 (60584521)
國井 政孝 大阪大学, 医学系研究科, 助教 (80614768)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | 細胞極性 / 極性輸送 / Rab / SNARE / 上皮細胞 / ノックアウトマウス |
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| Outline of Final Research Achievements |
SNAP23 pancreas-specific knockout (KO) mice showed decreased and increased secretion of amylase and insulin, respectively, which was published in JCB. Brain-specific SNAP23 KO mice showed defects in both cerebral and cerebellar cortex, which is being prepared for publication. We showed defects in apical transport in Rab11a KO mice, which was published in Biology Open.
We identified many genes involved in cell polarity. Two of them, Rab1a and BIG1, an activator of Arf, was knocked out in the mice and the results of BIG1 KO mice was published in Plos One. Other genes, such as Rab1, 11b, syntaxin3 were already knocked out and being analyzed. In addition, we identified novel binding proteins for Rab8 and Rab11. We analyzed their roles in vitro and in vivo and the results were published recently in JCB.
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