• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Morphofunctional analyses of autophagy for understanding the mechanism of the isolation membrane formation

Research Project

Project/Area Number 15H04670
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionFukushima Medical University

Principal Investigator

Satoshi Waguri  福島県立医科大学, 医学部, 教授 (30244908)

Co-Investigator(Kenkyū-buntansha) 甲賀 大輔  旭川医科大学, 医学部, 准教授 (30467071)
荒井 律子  福島県立医科大学, 医学部, 助教 (10342742)
田村 直輝  福島県立医科大学, 医学部, 助教 (70745992)
安納 弘道  福島県立医科大学, 医学部, 助教 (80258392)
植村 武文  福島県立医科大学, 医学部, 講師 (80548925)
亀高 諭  名古屋大学, 医学系研究科(保健), 教授 (10303950)
Co-Investigator(Renkei-kenkyūsha) KOMATSU MASAAKI  新潟大学, 医歯(薬)学総合研究科, 教授 (90356254)
USHIKI TATSUO  新潟大学, 医歯(薬)学総合研究科, 教授 (40184999)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywordsオートファジ―隔離膜 / CLEM / マイトファジー / 鉄欠乏 / 創傷治癒 / 線維芽細胞 / 癌カヘキシア / 筋委縮 / オートファジ― / 隔離膜 / 電子顕微鏡 / 細胞・組織 / オートファジー / 電子顕微鏡解析
Outline of Final Research Achievements

This study aimed to identify precursor structures of the autophagic isolation membrane. Because another group demonstrated the ones induced by amino acid starvation using similar approaches, we focused on investigating on mitophagy. As a results, we found that the extension of the isolation membrane on the mitochondrial surface is involved in the iron-deficiency induced mitophagy. Also, we were able to establish a method for observing the isolation membrane by scanning electron microscopy, which will promote future research project. Finally, we suggested the importance of autophagy-lysosomal system in fibroblasts during wound-healing process and in muscle fibers in cancer induced cachexia.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (26 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (15 results) (of which Int'l Joint Research: 1 results,  Invited: 7 results) Remarks (1 results)

  • [Int'l Joint Research] Institute of Biophysics, CAS(中国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] HMGB1 promotes ductular reaction and tumorigenesis in autophagy-deficient livers2018

    • Author(s)
      Khambu Bilon、Huda Nazmul、Chen Xiaoyun、Antoine Daniel J.、Li Yong、Dai Guoli、Kohler Ulrike A.、Zong Wei-Xing、Waguri Satoshi、Werner Sabine、Oury Tim D.、Dong Zheng、Yin Xiao-Ming
    • Journal Title

      Journal of Clinical Investigation

      Volume: - Issue: 6 Pages: 2419-2435

    • DOI

      10.1172/jci91814

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] GGA2 interacts with EGFR cytoplasmic domain to stabilize the receptor expression and promote cell growth2018

    • Author(s)
      Uemura Takefumi、Kametaka Satoshi、Waguri Satoshi
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1368-1368

    • DOI

      10.1038/s41598-018-19542-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spatiotemporal alterations of autophagy marker LC3 in rat skin fibroblasts during wound healing process2018

    • Author(s)
      Asai E, Yamamoto M, Ueda K, Waguri S
    • Journal Title

      FUKUSHIMA JOURNAL OF MEDICAL SCIENCE

      Volume: 64 Issue: 1 Pages: 15-22

    • DOI

      10.5387/fms.2016-13

    • NAID

      130006708191

    • ISSN
      0016-2590, 2185-4610
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] LC3-positive puncta increase in skeletal muscle of patient-derived xenograft mice2018

    • Author(s)
      安納 弘道、土橋 悠、田村 直輝、植村 武文、和栗 聡
    • Journal Title

      Japanese Journal of Physical Fitness and Sports Medicine

      Volume: 67 Issue: 1 Pages: 99-105

    • DOI

      10.7600/jspfsm.67.99

    • NAID

      130006315837

    • ISSN
      0039-906X, 1881-4751
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] オートファジー微細形態学 ―メゾスケールレベルの克服2017

    • Author(s)
      和栗聡
    • Journal Title

      実験医学

      Volume: 35 Pages: 2483-2490

    • Related Report
      2017 Annual Research Report
  • [Journal Article] p62/Sqstm1 promotes malignancy of HCV-positive hepatocellular carcinoma through Nrf2-dependent metabolic reprogramming2016

    • Author(s)
      Saito T, Ichimura Y, Taguchi K, Suzuki T, Mizushima T, Takagi K, Hirose Y, Nagahashi M, Iso T, Fukutomi T, Ohishi M, Endo K, Uemura T, Nishito Y, Okuda S, Obata M, Kouno T, Imamura R, Tada Y, Obata R, Yasuda D, Takahashi K, Fujimura T, Pi J, Lee MS, Ueno T, Ohe T, Mashino T, Wakai T, Kojima H, Okabe T, Nagano T et al.
    • Journal Title

      Nature Commun

      Volume: 7 Issue: 1 Pages: 12030-12030

    • DOI

      10.1038/ncomms12030

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Autophagy linked FYVE (Alfy/WDFY3) is required for establishing neuronal connectivity in the mammalian brain.2016

    • Author(s)
      Dragich JM, Kuwajima T, Hirose-Ikeda M, Yoon MS, Eenjes E, Bosco JR, Fox LM, Lystad AH, Oo TF, Yarygina O, Mita T, Waguri S, Ichimura Y, Komatsu M, Simonsen A, Burke RE, Mason CA, Yamamoto A.
    • Journal Title

      Elife

      Volume: 20

    • DOI

      10.7554/elife.14810

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Differing susceptibility to autophagic degradation of two LC3-binding proteins: SQSTM1/p62 and TBC1D25/OATL12016

    • Author(s)
      Hirano S, Uemura T, Annoh H, Fujita N, Waguri S, Itoh T, Fukuda M
    • Journal Title

      Autophagy

      Volume: 12 Pages: 312-326

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Sqstm1-GFP knock-in mice reveal dynamic actions of Sqstm1 during autophagy and under stress conditions in living cells2015

    • Author(s)
      Eino A, Kageyama S, Uemura T, Annoh H, Saito T, Narita I, Waguri S, Komatsu M
    • Journal Title

      Journal of Cell Science

      Volume: 128 Pages: 4453-4461

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] オートファジー関連遺伝子Atg2の生理機能解析2018

    • Author(s)
      和栗聡、坂井俊介、田村直輝、小松雅明
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 鉄キレート剤deferiprone誘導性マイトファジーにおける隔離膜形成過程の微細構造解析2018

    • Author(s)
      荒井律子、和栗聡
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 浸透圧ストレス誘導性オートファジーの分子メカニズムの解析2018

    • Author(s)
      田村直輝、和栗聡
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 浸透圧ストレス誘導性オートファジーの分子機構の解明2017

    • Author(s)
      田村直輝、和栗聡
    • Organizer
      第69回日本細胞生物学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Phenotypic analysis of mice defective in Atg2A/B2017

    • Author(s)
      Sakai S, Tamura N, Komatsu M., Waguri S
    • Organizer
      第69回日本細胞生物学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] オートファジー研究から小児成長を考える2017

    • Author(s)
      和栗聡
    • Organizer
      第18回東北小児成長フォーラム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Ultrastructure of Autophagy by Electron Microscopy2017

    • Author(s)
      Waguri S
    • Organizer
      The 25th International Symposia on Morphological Sciences
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 哺乳類細胞オートファゴソーム形成過程における膜構造変化の解析2017

    • Author(s)
      荒井律子、和栗聡
    • Organizer
      第63回日本解剖学会 東北・北海道連合支部学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] オートファジー研究の現状と皮膚科学における可能性2017

    • Author(s)
      和栗聡
    • Organizer
      第81回日本皮膚科学会東部支部学術大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] オートファジーの形態学 : 電顕と免疫組織2017

    • Author(s)
      和栗聡
    • Organizer
      第33回Wakoワークショップ
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] オートファジー関連分子WIPI-1、WIPI-2 の浸透圧ストレスへの関与2016

    • Author(s)
      田村直輝、和栗聡
    • Organizer
      第62回日本解剖学会 東北・北海道連合支部学術集会
    • Place of Presentation
      帯広
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] 構造化照明法によるオートファジー隔離膜の超解像顕微鏡解析2016

    • Author(s)
      和栗聡
    • Organizer
      第57回 日本組織細胞化学会総会・学術集会
    • Place of Presentation
      三鷹
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] オートファジー関連分子WIPI-1、WIPI-2 の浸透圧ストレスへの関与2016

    • Author(s)
      田村直輝、和栗聡
    • Organizer
      第68回日本細胞生物学会大会
    • Place of Presentation
      京都
    • Year and Date
      2016-06-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] Sqstm1-GFP knock-in mice reveal dynamic action of Sqstm1 / p62 during autophagy and stress-conditions in living cells2016

    • Author(s)
      Atushi Eino, Shun kageyama, Takefumi Uemura, Hiromichi Annoh, Tetsuya Saito, Ichiei Narita, ○ Satoshi Waguri, Masaaki Komatsu
    • Organizer
      第121回日本解剖学会総会全国学術集会
    • Place of Presentation
      郡山
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] オートファジー隔離膜の形成過程に関与する新規細管集合体2015

    • Author(s)
      植村武文、山本雅哉、安納弘道、小松雅明、和栗聡
    • Organizer
      第56回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      枚方
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Remarks] 福島県立医科大学 解剖・組織学講座 業績

    • URL

      http://www.fmu.ac.jp/home/anatomy2/achieve.html

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report 2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2022-08-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi