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Visualization of maternal separation memory engram and retrieval by activating positive memory engram

Research Project

Project/Area Number 15H04671
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionNara Medical University

Principal Investigator

Nishi Mayumi  奈良県立医科大学, 医学部, 教授 (40295639)

Co-Investigator(Kenkyū-buntansha) 堀井 謹子  奈良県立医科大学, 医学部, 講師 (80433332)
東 超  奈良県立医科大学, 医学部, 学内講師 (90326322)
Co-Investigator(Renkei-kenkyūsha) HAYASHI Yasunori  京都大学, 大学院医学研究科・医学部, 教授 (90466037)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords記憶の痕跡 / 光遺伝学 / 幼児虐待 / c-fos遺伝子プロモーター / ストレス / HPA-axis / 養育行動
Outline of Final Research Achievements

We investigated whether the memory of maternal separation (MS) during early life is recalled. In order to address this issue, we tried to manipulate the memory engram induced by MS by irreversibly labeling the activated neurons during MS and re-activate these neurons by using optogenetic method. Furthermore, we analyzed maternal behaviors in the offspring suffered from MS. We found that these female offspring showed disorder of maternal behaviors including nest making, retrieving, etc., which caused higher death rate (more than 85%) in the neonatal period than control group.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (41 results)

All 2018 2017 2016 2015 Other

All Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Acknowledgement Compliant: 4 results,  Open Access: 4 results) Presentation (26 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Journal Article] A Newly Defined Area of the Mouse Anterior Hypothalamus Involved in Septohypothalamic Circuit: Perifornical Area of the Anterior Hypothalamus, PeFAH2018

    • Author(s)
      Nishi M* and Horii-Hayashi N
    • Journal Title

      ACTA HISTOCHEMICA ET CYTOCHEMICA

      Volume: 51 Issue: 1 Pages: 1-8

    • DOI

      10.1267/ahc.17030

    • NAID

      130006405036

    • ISSN
      0044-5991, 1347-5800
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insights from extracellular matrix studies in the hypothalamus: structural variations of perineuronal nets and discovering a new perifornical area of the anterior hypothalamus.2017

    • Author(s)
      Horii-Hayashi N*,Sasagawa T,Nishi M
    • Journal Title

      Anatomical Science International

      Volume: 92 Issue: 1 Pages: 18-24

    • DOI

      10.1007/s12565-016-0375-5

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effects of early life adverse experiences on the brain: implications from maternal separation2017

    • Author(s)
      Nishi M*, Sasagawa T, Horii-Hayashi N
    • Journal Title

      Folia Pharmacologica Japonica

      Volume: 149 Issue: 2 Pages: 72-75

    • DOI

      10.1254/fpj.149.72

    • NAID

      130005306339

    • ISSN
      0015-5691, 1347-8397
    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Nitric Oxide Induced by Angiotensin II Receptor Type 1 Using Soluble Guanylate Cyclase beta1 Subunit Fused to a Yellow Fluorescent Protein, Venus.2017

    • Author(s)
      Tsuji Y, Ozawa K*, Komatsubara T, Zhao J, Nishi M, Yoshizumi M.
    • Journal Title

      J Fluoresc

      Volume: 27 Pages: 399-405

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural Reconstruction of the Perivascular Space in the Adult Mouse Neurohypophysis during an Osmotic Stimulation.2017

    • Author(s)
      Nishikawa K, Furube E, Morita-Takemura S, Horii-Hayashi N, Nishi M, Miyata S.
    • Journal Title

      J Neuroendocrinol

      Volume: 29 Issue: 2

    • DOI

      10.1111/jne.12456

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Long-term effects of maternal separation on reward seeking and dopamine D1 receptor expression via DNA methylation in female mice.2017

    • Author(s)
      Sasagawa T, Horii-Hayashi N, Okuda A, Hashimoto T, Azuma C, Nishi M*
    • Journal Title

      Neurosci Lett

      Volume: 641 Pages: 33-39

    • DOI

      10.1016/j.neulet.2017.01.025

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Bone marrow stromal cell sheets may promote axonal regeneration and functional recovery with suppression of glial scar formation after spinal cord transection injury in rats.2016

    • Author(s)
      Okuda A*, Horii-Hayashi N, Sasagawa T, Shimizu T, Shigematsu H, Iwata E, Morimoto Y, Masuda K, Koizumi M, Akahane M, Nishi M, Tanaka Y
    • Journal Title

      J Neurosurgery Spine

      Volume: 26 Issue: 3 Pages: 388-395

    • DOI

      10.3171/2016.8.spine16250

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Bioinspired design of a polymer gel sensor for the realization of extracellular Ca2+ imaging2016

    • Author(s)
      F. Ishiwari, H. Hasebe, S. Matsumura, F. Hajjaj, N. Horii-Hayashi, M. Nishi, T. Someya, T. Fukushima
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 24275-24275

    • DOI

      10.1038/srep24275

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] マウス脳に新たに同定した前視床下部脳弓周囲野PeFAHと情動神経回路の関わり2016

    • Author(s)
      堀井謹子、笹川誉世、西真弓
    • Journal Title

      BIO Clinica

      Volume: 31 Pages: 76-81

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new area of the mouse anterior hypothalamus involved in septohypothalamic circuit Interdisciplinary Inforamation Science2015

    • Author(s)
      Horii-Hayashi N, Sasagawa T, Nishi M*.
    • Journal Title

      Interdisciplinary Information Science

      Volume: 21 Pages: 243-251

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A newly identified mouse hypothalamic area having bidirectional neural connections with the lateral septum: the perifornical area of the anterior hypothalamus rich in chondroitin sulfate proteoglycans.2015

    • Author(s)
      Horii-Hayashi N, Sasagawa T, Hashimoto T, Kaneko T, Takeuchi K, Nishi M.
    • Journal Title

      Eur J Neurosci.

      Volume: 42(6) Issue: 6 Pages: 2322-2234

    • DOI

      10.1111/ejn.13024

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Developmental and structural variety of the chondroitin sulfate proteoglycans-contained extracellular matrix in the mouse brain2015

    • Author(s)
      Horii-Hayashi N, Sasagawa T, Matsunaga W, Nishi M
    • Journal Title

      Neural plasticity.

      Volume: 2015 Pages: 256389-256389

    • DOI

      10.1155/2015/256389

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 視床下部新規領域 PeFAH の Urocortin3/Enkephalin 共発現ニューロンの機能.2018

    • Author(s)
      堀井謹子、西真弓
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 集団飼育環境における自閉症モデルマウスの行動解析.2018

    • Author(s)
      遠藤のぞみ、牧之段学、堀井謹子、杣山奈実、小森崇史、岸本年史、西真弓
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] マウスにおける胎児期受動喫煙が摂食、情動行動に及ぼす影響.2018

    • Author(s)
      蜂谷奈都子、笹川誉世、堀井謹子、遠藤のぞみ、西真弓
    • Organizer
      第123回日本解剖学会総会・全国学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 視床下部新領域PeFAHの機能2017

    • Author(s)
      堀井謹子、笹川誉世、並河知宏、西真弓
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Quantitative changes of hippocampal selenium and plasma protein carbonyl in mice after maternal separation2017

    • Author(s)
      Cho Azuma, Takayo Sasagawa, Takeshi Minami, Mayumi Nishi
    • Organizer
      第122回日本解剖学会総会・全国学術集会
    • Place of Presentation
      長崎
    • Year and Date
      2017-03-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 新規行動解析システムによる社会的集団における自閉症モデルマウスの行動表現型解析2017

    • Author(s)
      遠藤のぞみ、牧之段学、堀井謹子、杣山奈実、小森崇史、岸本年史、西真弓
    • Organizer
      第93回日本解剖学会近畿支部学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Neural networks between a newly identified perifornical area of the anterior hypothalamus and lateral septum: chemogenetic investigations for physiological roles of urocortin3/enkephalin co-expressing neurons.2017

    • Author(s)
      Mayumi Nishi.
    • Organizer
      The 12th China-Japan Joint Seminar on Histichemistry and Cytochemistry.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ストレスの神経科学:コルチコステロイド受容体のリアルタイムイメージングから幼少期ストレスの研究まで.2017

    • Author(s)
      西真弓
    • Organizer
      第58回日本組織細胞化学会総会・学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Neural circuit controlling defensive active coping with inanimate threats.2017

    • Author(s)
      Noriko Horii, Takayo Sasagawa, Mayumi Nishi.
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 幼少期ストレスが嗜好性食餌に対する報酬行動に及ぼす影響について2016

    • Author(s)
      笹川誉世、堀井謹子、西真弓
    • Organizer
      第92回日本解剖学会近畿支部学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2016-11-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] Part 1: Effects of early life adverse experiences on the brain: Implications from maternal separation in rodents. Part 2: Neural networks between a newly identified perifornical area of the anterior hypothalamus and lateral septum: Chemogenetic investigations for physiological roles of urocortin3/enkephalin co-expressing neurons.2016

    • Author(s)
      Mayumi Nishi
    • Organizer
      3rd IBRO-APRC Advanced School of Neuroscience 2016.
    • Place of Presentation
      MALAYSIA
    • Year and Date
      2016-11-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 幼少期ストレスが嗜好性  食餌に対する欲求行動へ与える影響について2016

    • Author(s)
      笹川誉世、堀井謹子、並河知宏、西真弓
    • Organizer
      第43回日本神経内分泌学会学術集会
    • Place of Presentation
      浜松
    • Year and Date
      2016-10-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 視床下部の新たな機能―脳弓周囲野が制御する行動―2016

    • Author(s)
      堀井謹子、笹川誉世、並河知宏、西真弓
    • Organizer
      第43回日本神経内分泌学会学術集会
    • Place of Presentation
      浜松
    • Year and Date
      2016-10-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 幼少期ストレスが嗜好性食餌に対する欲求行動へ与える影響について2016

    • Author(s)
      笹川 誉世、堀井-林 謹子、西 真弓
    • Organizer
      第25回日本行動神経内分泌研 究会
    • Place of Presentation
      熱海
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] ペリニューロナルネット研 究から見えてきた臨界期の時空間パターンと視床下部細胞外マトリ クスの多様性2016

    • Author(s)
      堀井謹子、笹川誉世、並河知宏、西真弓
    • Organizer
      第57回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      東京
    • Year and Date
      2016-09-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] 幼少期ストレスによる食物嗜好性行動への影響について2016

    • Author(s)
      笹川 誉世、堀井-林 謹子、奥田 哲教、西 真弓
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] 視床下部領域脳弓周囲野-外側中隔神経回路の役割:ウロコルチン3/エンケファリン共発 現ニューロンの薬理遺伝学的解析2016

    • Author(s)
      堀井 謹子、笹川 誉世、並河 知宏、西 真弓
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス脳に新たに発見・同定した視床下部領域について.2016

    • Author(s)
      堀井謹子、笹川誉世、橋本隆、西真弓
    • Organizer
      第121回日本解剖学界総会・全国学術集会
    • Place of Presentation
      福島
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] サル輪状軟骨の元素蓄積の特徴.2016

    • Author(s)
      東超、大石高生、東野義之、東野勢津子、南武志、西真弓
    • Organizer
      第121回日本解剖学界総会・全国学術集会
    • Place of Presentation
      福島
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effects of early life stress on the brain.2016

    • Author(s)
      Mayumi Nishi, Takayo Sasagawa, Noriko Horii-Hayashi.
    • Organizer
      第89回日本薬理学会年会 シンポジウム
    • Place of Presentation
      横浜
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 幼少期ストレスによる嗜好性食餌に対する摂食・欲求行動への影響について.2015

    • Author(s)
      笹川誉世、堀井謹子、奥田哲教、西真弓
    • Organizer
      第56回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2015-10-03
    • Related Report
      2015 Annual Research Report
  • [Presentation] 幼少期ストレスによる嗜好性食餌に対する摂食・欲求行動への影響について.2015

    • Author(s)
      笹川誉世、堀井謹子、奥田哲教、西真弓
    • Organizer
      第42回日本神経内分泌学会・第23回日本行動神経内分泌研究会合同学術集会
    • Place of Presentation
      仙台
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effects of early life adverse experiences on the brain.2015

    • Author(s)
      Mayumi Nishi, Noriko Horii-Hayashi, Takayo Sasagawa
    • Organizer
      第42回日本神経内分泌学会企画国際シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2015-09-18
    • Related Report
      2015 Annual Research Report
  • [Presentation] A new hypothalamic area enriched with perineuronal nets having bidirectional connections between the lateral septum.2015

    • Author(s)
      Mayumi Nishi, Noriko Horii-Hayashi, Takayo Sasagawa
    • Organizer
      Parvo-and Magnocellular Symposium in Sendai―Creating a New Stream of Neuroendocrinology
    • Place of Presentation
      仙台
    • Year and Date
      2015-09-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] A newly identified hypothalamic area enriched with perineuronal net-positive neurons.2015

    • Author(s)
      Horii, Takayo Sasagawa, Takashi Hashimoto, Mayumi Nishi
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effects of early life stress on feeding behavior for palatable foods.2015

    • Author(s)
      Takayo Sasagawa, Noriko Horii, Mayumi Nishi
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Book] スタンフォード神経生物学2017

    • Author(s)
      西真弓 翻訳(分担)
    • Total Pages
      705
    • Publisher
      メデイカル・サイエンス・インターナショナル
    • ISBN
      9784895928885
    • Related Report
      2017 Annual Research Report
  • [Remarks] 奈良県立医科大学第一解剖学講座

    • URL

      http://www.naramed-u.ac.jp/~1ana/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 奈良県立医科大学 第一解剖学講座

    • URL

      http://www.naramed-u.ac.jp/~1ana/

    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2019-03-29  

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