Study on regulation mechanisms of cardiac rhythm and energy metabolism via mitochondria-sarcoplasmic reticulum coupling
| Project/Area Number |
15H04674
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
竹内 綾子 福井大学, 学術研究院医学系部門, 准教授 (00378704)
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| Co-Investigator(Renkei-kenkyūsha) |
HIKIDA Masaki 秋田大学, 大学院理工学研究科, 教授 (60228715)
SHIMAYOSHI Takao 九州大学, 情報基盤研究開発センター, 准教授 (60373510)
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| Research Collaborator |
IINO Satoshi 福井大学, 学術研究院医学系部門, 教授 (40242854)
HORIGUCHI Kazuhide 福井大学, 学術研究院医学系部門, 准教授 (20377451)
FUKAZAWA Yugo 福井大学, 学術研究院医学系部門, 教授 (60343745)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Keywords | ミトコンドリア / カルシウム / 心臓 / 数理モデル / 心臓生理 / トランスポータ / 心臓生理学 / モデル |
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| Outline of Final Research Achievements |
NCLX-knockout mice were created using the CRISPR-Cas9 method. Some of the homo-knockout mice show phenotypes different form wild type mice, such as low body weight, and abnormalities of heart and kidney. The Langendorff perfusion of mouse heart with a NCLX blocker (CGP-37157) tended to have decreased heart rate. In isolated mouse ventricular myocytes, CGP-37157 prolonged the 30 and 50% duration of action potential, and augmented the amplitude of Ca2+ transient probably due to the increase of SR Ca2+ content. A new comprehensive mathematical model of cardiac mitochondria was developed and published. A new mathematical model of sinoatrial cell, which contains the Ca2+ interaction between mitochondria and SR, was developed.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Patient-Specific Human Induced Pluripotent Stem Cell Model Assessed with Electrical Pacing Validates S107 as a Potential Therapeutic Agent for Catecholaminergic Polymorphic Ventricular Tachycardia2016
Author(s)
Sasaki K, Makiyama T, Yoshida Y, Wuriyanghai Y, Kamakura T, Nishiuchi S, Hayano M, Harita T, Yamamoto Y, Kohjitani H, Hirose S, Chen J, Kawamura M, Ohno S, Itoh H, Takeuchi A, Matsuoka S, Miura M, Sumitomo N, Horie M, Yamanaka S, Kimura T
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Journal Title
PLoS One
Volume: 11
Issue: 10
Pages: e0164795-e0164795
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Dysregulation of a potassium channel, THIK-1, targeted by caspase-8 accelerates cell shrinkage2016
Author(s)
Sakamaki K, Ishii TM, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Jung SK, Matsuoka S, Koyamada K, Sawasaki T, Nagai T, Ueno N
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Journal Title
Biochim Biophys Acta
Volume: 1863
Issue: 11
Pages: 2766-2783
DOI
Related Report
Peer Reviewed
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