| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
|
|---|
| Outline of Final Research Achievements |
Circadian clocks reside in each cell-level throughout the body in mammals. Intrinsic cellular circadian clocks develop cell-autonomously during the cellular differentiation process. However, mechanisms controlling the emergence of cellular circadian clock oscillation in vivo have not yet been fully understood. Here, we show that Dicer/Dgcr8-mediated post-transcriptional mechanisms control the CLOCK protein expression in both mouse fetal hearts and in vitro differentiating ESCs, which contributes to the emergence of circadian clock in mammalian cells. This event occurs after cell lineage determination into hearts or loss of pluripotent stem cell markers in differentiating ESCs, suggesting the cellular differentiation-coupled clock development may be conducted by a two-step program consisting of cellular differentiation and following establishment of circadian transcriptional / translational feedback loops.
|
