• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional analysis of DOPAergic transmission in cardiovascular system

Research Project

Project/Area Number 15H04687
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionYokohama City University

Principal Investigator

GOSHIMA Yoshio  横浜市立大学, 医学研究科, 教授 (00153750)

Co-Investigator(Kenkyū-buntansha) 古賀 資和  横浜市立大学, 医学部, 助教 (00637233)
中村 史雄  東京女子医科大学, 医学部, 教授 (10262023)
増川 太輝  横浜市立大学, 医学部, 助手 (10711898)
及川 雅人  横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 教授 (70273571)
Co-Investigator(Renkei-kenkyūsha) FUKAZAWA Yugo  福井大学, 医学部, 教授 (60343745)
ICHINOSE Hiroshi  東京工業大学, 生命理工学研究科, 教授 (90192492)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywords生理活性物質 / ドーパ / 血圧 / 日内変動 / 血管平滑筋 / α1アドレナリン受容体 / Gタンパク質連関型受容体 / 眼白子症原因遺伝子
Outline of Final Research Achievements

We proposed that L-3,4-dihydroxyphenylalanine (DOPA) is a neurotransmitter. We recently identified OA1/GPR143, the gene product of ocular albinism 1, as a receptor candidate for DOPA. However, physiological relevance of DOPA action mediated through GPR143 remains unknown. To examine the role of GPR143, we established Gpr143-gene deficient (GPR143-KO) mice. We found that cardiovascular responses to intravenous α1-adrenergic receptor (α1AR) agonist phenylephrine were attenuated in GPR143-KO mice compared to wild-type (Wt) mice. DOPA at nanomolar concentrations alone produced no effect, but it enhanced phenylephrine-induced vasoconstriction in isolated descending aorta. In Wt mice, blood pressure increased during the transition from light-rest to dark-active phases. This elevation was not observed in GPR143-KO mice. Our findings provide evidence that DOPA/GPR143 signaling controls sympathetic neurotransmission through sensitizing vascular α1AR.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (25 results)

All 2017 2016 2015 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (21 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (2 results)

  • [Journal Article] L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor2017

    • Author(s)
      Masukawa Daiki、Koga Motokazu、Sezaki Anna、Nakao Yuka、Kamikubo Yuji、Hashimoto Tatsuo、Okuyama-Oki Yuki、Aladeokin Aderemi Caleb、Nakamura Fumio、Yokoyama Utako、Wakui Hiromichi、Ichinose Hiroshi、Sakurai Takashi、Umemura Satoshi、Tamura Koichi、Ishikawa Yoshihiro、Goshima Yoshio
    • Journal Title

      JCI Insight

      Volume: 2 Issue: 18 Pages: 1-16

    • DOI

      10.1172/jci.insight.90903

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] l-3,4-Dihydroxyphenylalanine induces ptosis through a GPR143-independent mechanism in mice.2016

    • Author(s)
      Ueda S, Masukawa D, Koga M, Goshima Y
    • Journal Title

      J Pharmacol Sci

      Volume: 132 Issue: 1 Pages: 109-112

    • DOI

      10.1016/j.jphs.2016.08.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] L-DOPA neurotransmitter hypothesis2017

    • Author(s)
      Goshima Y
    • Organizer
      第90回日本薬理学会年会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] DOPA-GPR143 signal augments α1-adrenergic receptor response2017

    • Author(s)
      Masukawa D, Nakamura F, Yokoyama U, Koga M, Ishikawa Y, Goshima Y
    • Organizer
      第90回日本薬理学会年会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] GPR143 is involved in dopamine D2 receptor-mediated transmission in mice2017

    • Author(s)
      Kitamura S, Masukawa D, Koga M, Nakamura F, Goshima Y
    • Organizer
      第90回日本薬理学会年会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] ドーパ受容体GPR143とアドレナリンα1受容体との機能的連関と複合体形成.2017

    • Author(s)
      増川太輝, 古賀資和, 涌井広道, 上窪裕二, 横山詩子, 中村史雄, 櫻井隆, 田村功一, 石川義弘, 五嶋良郎
    • Organizer
      第136回日本薬理学会関東部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] L-DOPAの肺高血圧モデル肺血管におけるフェニレフリン応答の修飾作用2017

    • Author(s)
      古賀資和, 増川太輝, 中村史雄, 菅原陽, 水野祐介, 後藤隆久, 五嶋良郎
    • Organizer
      第136回日本薬理学会関東部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] L- DOPA sensitizes nervous system by modulating the vascular α1-adrenergic receptor.2017

    • Author(s)
      Masukawa D, Koga M, Wakui H, Kamikubo Y, Yokoyama U, Nakamura F, Tamura K, Umemura S, Sakurai T, Ishikawa Y, Goshima Y
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Immunoreactive signals of GPR 143 in ventral tegmental areas and its trajectory.2017

    • Author(s)
      Koga M, Masukawa D, Nakamura F, Goshima Y
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Screening for L-DOPA ligands by monitoring central cardiovascular regulation in the nucleus tractus solitarii.2017

    • Author(s)
      Nakao Y, Masukawa D, Koga M, Goshima Y
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] L-DOPA受容体候補分子GPR143のマウス腹側被蓋野及びその投射野における分布.2017

    • Author(s)
      瀧澤光太郎, 古賀資和, 増川太輝, 中村史雄, 五嶋良郎
    • Organizer
      第137回日本薬理学会関東部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] GPR143遺伝子欠損マウスにおけるメチルフェニデート, コカイン及びニコチンの行動に及ぼす効果.2017

    • Author(s)
      金井香央里, 増川太輝, 古賀資和, 北村慧, 角田貴大, 五嶋良郎
    • Organizer
      第137回日本薬理学会関東部会
    • Related Report
      2017 Annual Research Report
  • [Presentation] GPR143, the gene product of ocular albinism 1(OA1), mediates actions of L-DOPA in the cardiovascular system.2016

    • Author(s)
      Goshima Y
    • Organizer
      9th World Symposium on Ocular Albinism
    • Place of Presentation
      SanDiego, CA (USA)
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ドーパ受容体GPR143はアドレナリンα1受容体応答を増強する.2016

    • Author(s)
      増川太輝、古賀資和、横山詩子、上窪裕二、中村史雄、櫻井隆、石川義弘、五嶋良郎
    • Organizer
      第135回日本薬理学会関東部会
    • Place of Presentation
      アクトシティ浜松(静岡県浜松市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] 中枢神経系における抗ドーパ抗体を用いた免疫組織化学的検討.2016

    • Author(s)
      増川太輝、ジョンソン実歌、古賀資和、中村史雄、五嶋良郎
    • Organizer
      第135回日本薬理学会関東部会
    • Place of Presentation
      アクトシティ浜松(静岡県浜松市)
    • Year and Date
      2016-10-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] Distribution of GPR143-immunoreactive cells in the mouse ventral tegmental area.2016

    • Author(s)
      Motokazu Koga, Daiki Masukawa, Fumio Nakamura, Yoshio Goshima
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] DOPA-GPR143 signal augments pressor response mediated by a1-adrenergic receptor.2016

    • Author(s)
      Daiki Masukawa, Fumio Nakamura, Utako Yokoyama, Motokazu Koga, Yoshihiro Ishikawa, Yoshio Goshima
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] Functional coupling between GPR143, a DOPA receptor, and alpha1-adrenergic receptor regulates peripheral sympathetic nerve transmission.2016

    • Author(s)
      Daiki Masukawa, Anna Sezaki, Fumio Nakamura, Motokazu Koga, Yoshio Goshima
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] The effect of dopamine receptors agonists in gpr143-/y mice.2016

    • Author(s)
      Satoshi Kitamura, Daiki Masukawa, Motokazu Koga, Fumio Nakamura, Yoshio Goshima
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] General behavioral test in the gpr143-/y mice.2016

    • Author(s)
      Hiromi Okatsu, Junka Koyama, Haruko Nakamura, Daiki Masukawa, Motokazu Koga, Fumio Nakamura, Tohru Kimura, Yoshio Goshima
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] GPR143遺伝子欠損マウスにおけるドパミン受容体作動薬の効果2015

    • Author(s)
      北村慧、増川太輝、古賀資和、中村史雄、五嶋良郎
    • Organizer
      第133回日本薬理学会関東部会
    • Place of Presentation
      柏の葉カンファレンスセンター(千葉県柏市)
    • Year and Date
      2015-10-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] Distribution and function of L-DOPA receptor, ocular albinism 1(OA-1), in the central nervous system.2015

    • Author(s)
      Daiki Masukawa, Fumio Nakamura, Yoshio Goshima
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場、神戸国際展示場(兵庫県神戸市)
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] L-DOPAによって誘発される行動薬理学的変化の解析2015

    • Author(s)
      上田傑、増川太輝、古賀資和、中村史雄、五嶋良郎
    • Organizer
      第132回日本薬理学会関東部会
    • Place of Presentation
      明海大学浦安キャンパス(千葉県浦安市)
    • Year and Date
      2015-07-04
    • Related Report
      2015 Annual Research Report
  • [Remarks] 横浜市立大学薬理学教室ホームページ

    • URL

      http://www-user.yokohama-cu.ac.jp/~pharmac/index.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 横浜市立大学薬理学教室ホームページ

    • URL

      http://www-user.yokohama-cu.ac.jp/~pharmac/

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi