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Roles of HIston methyation in retinal defferentiation and maintenance

Research Project

Project/Area Number 15H04695
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

Watanabe Sumiko  東京大学, 医科学研究所, 特任教授 (60240735)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords網膜 / エピジェネティクス / ヒストンメチル化 / 発生 / マウス / 分化 / 細胞系列 / 視細胞 / ヒストン / メチル化 / ヒストン修飾
Outline of Final Research Achievements

Ezh2 knockout mice retinas showed delay of development of not only photoreceptors, but also other neurons and glias. Therefore, roles of histone H3K27 methylation was suggested to be regulation of timing of cells differentiation. We found histone H3 lysine other than K27 was also involved in retinal development. ChIP-seq showed that the gene loci, which are highly modified this methylation, was also methylated in histone H3K27, suggesting new mechanisms that co-operated regulation of transcription by these two methylations.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (15 results)

All 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 2 results,  Invited: 7 results)

  • [Int'l Joint Research] National Institute of the Eye(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Texas at Austin(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Pivotal roles of Fezf2 in differentiation of cone OFF bipolar cells and functional maturation of cone ON bipolar cells in retina.2018

    • Author(s)
      Suzuki-Kerr H, Iwagawa T, Sagara H, Mizota A, Suzuki Y, Watanabe S
    • Journal Title

      Exp Eye Res

      Volume: 171 Pages: 142-154

    • DOI

      10.1016/j.exer.2018.03.017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Docosahexaenoic acid preserves visual function by maintaining correct disc morphology in retinal photoreceptor cells2017

    • Author(s)
      Shindou Hideo、Koso Hideto、Sasaki Junko、Nakanishi Hiroki、Sagara Hiroshi、Nakagawa Koh M.、Takahashi Yoshikazu、Hishikawa Daisuke、Iizuka-Hishikawa Yoshiko、Tokumasu Fuyuki、Noguchi Hiroshi、Watanabe Sumiko、Sasaki Takehiko、Shimizu Takao
    • Journal Title

      Journal of Biological Chemistry

      Volume: 292 Issue: 29 Pages: 12054-12064

    • DOI

      10.1074/jbc.m117.790568

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of M?ller glia specific genes and their histone modification using Hes1-promoter driven EGFP expressing mouse2017

    • Author(s)
      Ueno Kazuko、Iwagawa Toshiro、Ochiai Genki、Koso Hideto、Nakauchi Hiromitsu、Nagasaki Masao、Suzuki Yutaka、Watanabe Sumiko
    • Journal Title

      Sci Rep

      Volume: 7 Issue: 1 Pages: 3578-3578

    • DOI

      10.1038/s41598-017-03874-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Forkhead box protein P1 is essential for lens epithelium transition to the fiber cells during development but does not play roles for retinal development.2017

    • Author(s)
      Suzuki-Kerr H, Baba Y, Tsuhako A, Koso H, Dekker JD, Tucker H, Kuribayashi H, Watanabe S.
    • Journal Title

      Invest. Ophthalmol. Vis. Sci.

      Volume: 58 Issue: 4 Pages: 1916-1929

    • DOI

      10.1167/iovs.16-20085

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transition of differential histone H3 methylation in photoreceptors and other retinal cells during retinal differentiation2016

    • Author(s)
      Ueno Kazuko、Iwagawa Toshiro、Kuribayashi Hiroshi、Baba Yukihiro、Nakauchi Hiromitsu、Murakami Akira、Nagasaki Masao、Suzuki Yutaka、Watanabe Sumiko
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1

    • DOI

      10.1038/srep29264

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of retinal development via the epigenetic modification of histone H3.2016

    • Author(s)
      Watanabe, S., Murakami A
    • Journal Title

      Adv Exp Med Biol

      Volume: 854 Pages: 635-641

    • DOI

      10.1007/978-3-319-17121-0_84

    • ISBN
      9783319171203, 9783319171210
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 視細胞変性の分子基盤の解明を目指して2017

    • Author(s)
      渡辺すみ子
    • Organizer
      蛋白研セミナー「網膜感覚研究のフロンティア」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] EYS遺伝子変異が引き起こす網膜色素変性症のメカニズムの解明と 創薬戦略の構築2017

    • Author(s)
      渡辺すみ子
    • Organizer
      JRPS日本色素変性症協会全国大会「もうまくの日」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 網膜の発生と維持に関わるヒストンメチル化の役割2017

    • Author(s)
      渡辺すみ子
    • Organizer
      第237回つくばブレインサイエンス・セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Modeling of retinal degeneration and its application to develop therapeutic strategy2017

    • Author(s)
      渡辺すみ子
    • Organizer
      The 4th IMSUT-CGCT symposium
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Modeling of retinal degeneration and its application to develop therapeutic strategy2017

    • Author(s)
      渡辺すみ子
    • Organizer
      WIS-SIMS symposium (Weismann institute)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Regulation of retinal differentiation by histone methylation2016

    • Author(s)
      渡辺すみ子
    • Organizer
      Soonchunhyang University International Seminar
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 視細胞変性の分子基盤の解明を目指して2016

    • Author(s)
      渡辺すみ子
    • Organizer
      順天堂眼アレルギー研究会
    • Related Report
      2016 Annual Research Report
    • Invited

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Published: 2015-04-16   Modified: 2019-03-29  

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