Conditional TRIC-B knockout mice production and functional analysis
| Project/Area Number |
15H04698
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
Yamazaki Daiju 国立医薬品食品衛生研究所, 薬理部, 室長 (40467428)
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| Research Collaborator |
Goto Motohito
Takahashi Riichi
Yamamoto Shinichiro
Yamamura Hisao
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Keywords | TRICチャネル / 小胞体 / カウンターイオン / カウンターイオンチャネル / TRIC / カルシウム / ノックアウトマウス / コンディショナルノックアウト / TRIC-B / コンディショナル |
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| Outline of Final Research Achievements |
To investigate the TRIC-B function in various cells and tissues, we generated conditional TRIC-B deficient mice instead of conventional TRIC-B knockout mice which died immediately after birth due to impairement of pulmonary alveolous. First, we generated heart-specific TRIC-B deficient mice. Second, we generated mTRIC-B deficient ES cells and differntiated to the central nervous system cell lineage using with the neurosphere method. In heart-specific TRIC-B deficient mice, treatment of tamoxifen decreased gene expression of Tric-b in the heart. In addition, it was suggested that lack of TRIC-B may contribute to the differentiation process to the central nervous system cell lineage.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、心臓特異的TRIC-B欠損マウスを作製し、機能解析を目指した。残念ながら機能解析は行えなかったが、今後、心臓におけるTRIC-Bの機能が明らかになってくると期待される。また、中枢神経系を始めとした他の組織・細胞においてもTRIC-Bは多くの生理的役割を担っていることが推測されることから、TRIC-Bの生物学的意義の解明にとどまらず、TRIC-Bが原因となる疾患の発症機序解明や治療薬開発など臨床的な応用も期待できる。
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Report
(5 results)
Research Products
(14 results)
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[Journal Article] Mice lacking the intracellular cation channel TRIC-B have compromised collagen production and impaired bone mineralization.2016
Author(s)
C.Zhao, A.Ichimura, N.Qian, T.Iida, D.Yamazaki, N.Noma, M.Asagiri, K.Yamamoto, S.Komazaki, C.Sato, F.Aoyama, A.Sawaguchi, S.Kakizawa, M.Nishi, and H.Takeshima.
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Journal Title
science signaling
Volume: 9
Issue: 428
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Zinc Binding to MG53 Facilitates Repair of Injury to Cell Membrane2016
Author(s)
Peihui Lin, Chuanxi Cai, Hua Zhu, Jae-Kyun Ko, Moonsun Hwang, Zui Pan, Tao Tan, Daiju Yamazaki, Hiroshi Takeshima, Irina Korichneva, Jianjie Ma
Organizer
60th Biophysical Society Annual Meeting
Place of Presentation
米国、ロサンゼルス
Year and Date
2016-02-26
Related Report
Int'l Joint Research
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