| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
This study was conducted to investigate the possibility of Plasmodium falciparum gamete surface protein (Pf75) as a transmission-blocking vaccine candidate antigen. Twelve kinds of recombinant Pf75 were synthesized using a wheat germ cell-free protein synthesis system. To produce antiserum, a rabbit was immunized with each recombinant protein as an antigen. Three antisera showed strong reactivity to gametes of Plasmodium falciparum with immunofluorescence assay. Transmission-blocking efficacy was tested using standard membrane-feeding assay. The number of oocysts formed in the midgut of mosquitoes fed with infected erythrocytes mixed with anti-Pf75 antiserum or anti-GST antiserum was measured. A significant decrease in the number of oocysts was observed in the anti-Pf75 antiserum treated group compared to the anti-GST antiserum treated group (control), suggesting that the anti-Pf75 antibody has transmission-blocking activity.
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