| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2015: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Outline of Final Research Achievements |
Immunoglobulin A (IgA) is the main antibody isotype secreted into the intestinal lumen. IgA plays a critical role in the defense against pathogens and in the maintenance of intestinal homeostasis through gut microbial control. Our main question is what kind of bacterial molecule intestinal high-affinity IgA recognizes and targets. To address this question, we generated hybridomas from IgA producing cells in the small intestine of wild type mice. We selected W27 IgA that binds to multiple bacteria but not beneficial ones such as Lactobacillus casei. Via specific recognition of an epitope in serine hydroxymethyltransferase (SHMT), a bacterial metabolic enzyme, W27 IgA selectively inhibited the in vitro growth of Escherichia coli (E. coli), while having no effect on unbound beneficial bacteria such as L. casei. It indicates that W27 IgA has an ability to improve the intestinal environment.
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