| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Our former study demonstrated that the 3’ untranslated region (UTR) of HCV genome functions as a cis-acting element for the viral RNA packaging; however, the detailed functional mechanisms of packaging signal still remain unclear. In this study, mutations within the conserved stem-loops of the 3’ UTR were observed to diminish packaging efficiency, suggesting the conserved apical loop motifs of the 3´ end-side of 3’ UTR are important for the packaging, A foreign reporter gene flanked by the 3’ UTR was encapsidated by supplying both viral NS3-NS5B proteins and Core-NS2 in trans. The 3’ UTRs derived from GT-2a and -1a supported the packaging at comparable level. It is highly likely that specific recognition of the HCV genome via the packaging signal and coupling with replication are not mutually exclusive, but rather cooperate for the best beneficial of encapsidation and nuclecapsid formation.
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